Pengaruh Rizobakteri dalam Meningkatkan Kandungan Asam Salisilat dan Total Fenol Tanaman terhadap Penekanan Nematoda Puru Akar

Abstract

<p> </p><p>Penyakit puru akar pada tanaman kenaf (Hibiscus cannabinus L.) yang disebabkan oleh nematoda Meloidogyne spp. mengakibatkan penurunan kualitas dan kuantitas serat. Kolonisasi rizobakteri dalam rizosfer berperan sebagai antagonis yang dapat dimanfaatkan dalam ketahanan tanaman terhadap patogen. Peran rizobakteri sebagai bioprotektan dapat menurunkan populasi nematoda yang akan mempengaruhi perkembangan patogen penyebab penyakit. Penelitian ini bertujuan untuk mengevaluasi rizobakteri yang potensial dalam meningkatkan ketahanan tanaman kenaf terhadap infeksi nematoda Meloidogyne spp. melalui pembentukan metabolit sekunder diantaranya kandungan total fenol dan asam salisilat. Aplikasi<br /> rizobakteri dengan cara perendaman dan tanpa perendaman baik secara tunggal maupun konsorsium. Rizobakteri yang digunakan terdiri dari 3 jenis yaitu Pseudomonas fluorescens, Bacillus subtilis, dan Azotobacter sp. Pengamatan kandungan total fenol dan asam salisilat diamati pada 15 dan 25 hari setelah inokulasi dengan menggunakan alat spektrofotometer. Peningkatan total fenol dan asam salisilat tertinggi diperoleh ketika benih kenaf direndam dengan bakteri P. fluorescens berturut-turut sebesar 513,45% dan 235,99%. Terdapat peningkatan bobot kering tanaman kenaf dengan aplikasi rizobakteri dibandingkan dengan kontrol.</p><p> </p><p><strong><em>Effect </em></strong><strong><em>of Rhizobacteria in Content of Salicylic Acid and </em></strong><strong><em>Total Phenol </em></strong><strong><em>of Kenaf </em></strong><strong><em>Against </em></strong><strong><em>Nematodes</em></strong><strong><em> Infections</em></strong></p><p align="center"> </p><p><em>Root knoot disease of kenaf caused by nematodes Meloidogyne spp. is an important disease since it lowers quality and quantity of the fiber. Colonization of rhizobacteria in rhizosphere acts as an antagonist that can be utilized in plant resistance to pathogens. The role of rhizobacteria as a bioprotectan could reduce nematode population, and thus affect development of the disease. This study aimed to evaluate the potency of rhizobacteria in improving kenaf resistance against root knot nematode by inhibiting the production of total phenols and salicylic acid. Application of rhizobacteria was done by soaking or without soaking kenaf seeds either singly or in consortium. There were three rhizobacteria used in this study, i.e: Pseudomonas fluorescens, Bacillus subtilis, and Azotobacter sp. The content of total phenols and salicylic acid was observed at 15 and 25 days after inoculation using a spectrophotometer. The highest elevation level of total phenols and salicylic acid was obtained when kenaf seeds were soaked in P. fluorescens 513,45% and 235,99% respectively. There is an increase dry weight of kenaf with aplication of rhizobacteria compared with controls.</em></p>

Host Range Pantoea Ananatis the Causal Agent of Bacterial Leaf Blight on Allium Spp.

“…Therefore the pathogen suppressed slower and eventually the disease symptoms rapidly appear. The formation of phenol compounds, peroxidase, and salicylic acid as active defense responses of plants against pathogenic have been deported by Wijayanti et al (2017). The accumulation of phenolic compounds in leaf tissue of plants is often associated with plant resistance to disease (Hua et al, 2014).…”

Probability Induction of Kratom Plant Bioactive Components in Antidiabetic and Antiobesity Studies

“…Plant response is shown through genes that play a role in plant resistance to certain compounds, one of which is phenols. In addition to producing phenols, the activation of defence compounds is indicated by producing jasmonic acid and ethylene compounds (Wijayanti et al, 2018). The administration of non-pathogenic bacteria pseudomonas fluorescens has proven capable of accumulating the output of phenol compounds in plants (Anita et al, 2004).…”

PENGARUH MIKORIZA TERHADAP PENYAKIT LAYU FUSARIUM (Fusarium oxysporum) PADA TEMBAKAU (Nicotiana tabacum L.) DALAM MEDIA PASIR KUARSA MENGANDUNG KOMPOS AMB-P0K

“…Skoring penyakit berdasarkan Gao et al (1994) : 0 = Tanaman tidak terserang penyakit, 1 = 1 -25 % daun tanaman terserang layu fusarium, 2= 26 -50 % daun tanaman terserang layu fusarium, 3 = 51 -75 % daun tanaman terserang layu fusarium, 4 = 75 % -100% tanaman terserang layu fusarium. Berikut ini adalah rumus intensitas penyakit: (Widjayanti, 2007). Larutan induk asam salisilat dibuat dengan konsentrasi 0.1 mg/ml dan selanjutnya digunakan untuk membuat larutan standart asam salisilat 0.001%.…”