Pengaruh Sumber Spermatofora Pada Inseminasi Buatan Induk Betina Udang Windu Turunan Pertama (F-1) Terhadap Pemijahan, Kualitas Telur, Dan Larva Turunannya (F-2)

Abstract

Penelitian ini bertujuan mengevaluasi pemijahan udang windu betina F-1 dan mutu telur, serta larva turunannya (F-2) dengan inseminasi buatan menggunakan sumber spermatofora udang jantan yang berbeda. Perlakuan yang dicobakan adalah induk udang windu betina F-1 diinseminasi menggunakan spermatofora dari sumber induk jantan berbeda yaitu: spermatofora induk jantan F-1 hasil budidaya (S-1), dan spermatofora induk jantan alam (S-A). Data pemijahan induk betina, kualitas telur, dan profil asam amino pada daging dan hepatopankreas jantan, serta morfologi larva dianalisis secara deskriptif, sedangkan data uji vitalitas larva dianalisis uji t. Hasil penelitian menunjukkan bahwa induk udang windu betina F-1 yang diinseminasi dengan perlakuan S-1 memiliki tingkat pemijahan 67%; fekunditas 179.257 butir/induk; total produksi telur 1.434.053 butir; tingkat pembuahan telur 86,2%; daya tetas telur 59,8%; dan total produksi nauplii 738.439 ekor yang lebih rendah dibandingkan pada induk udang F-1 yang diinseminasi perlakuan S-A yang memiliki tingkat pemijahan 75%; fekunditas 215.489 butir/induk; total produksi telur 1.939.399 butir; tingkat pembuahan telur 88,9%; daya tetas telur 62,7%; dan total produksi nauplii 1.081.140 ekor. Sementara diameter telur (248-255 µm) dan mutu larva relatif sama di antara kedua perlakuan. Profil asam amino hepatopankreas dan daging pada induk udang jantan alam lebih tinggi dibandingkan pada induk udang jantan F-1. Penggunaaan spematofora jantan alam masih lebih baik daripada jantan budidaya pada inseminasi induk betina F-1 udang windu.This study was aimed at evaluating the spawning rate of F-1 female tiger shrimp and the quality of their egg and larvae derivatives (F-2) by artificial insemination using different sources of male shrimp spermatophore. The treatments consisted of broodstock of F-1 female tiger shrimp inseminated with different male spermatophores, namely: cultivated F-1 male spermatophore (S-1), and wild male spermatophore (S-A). Data on spawning performance of F-1 female tiger shrimp and amino acid profile in the hepatopancreas and muscle of male tiger shrimp and larval morphology were analyzed descriptively.The vitality of larvae was analyzed using t-test. The results showed that the broodstock of F-1 female tiger shrimp inseminated with S-1 treatment had spawning rate of 67%; fecundity of 179,257 egg; total egg production of 1,434,053 eggs; egg fertilization rate of 86.2%; hatching rate of 59.8%; and total nauplii production of 738,439 ind. Broodstock of F-1 female inseminated with S-A treatment had higher values for spawning rate of 75%, fecundity of 215,489 egg; total egg production of 1,939,399 egg; egg fertilization rate of 88.9%; hatching rate of 62.7%; and total nauplii production of 1,081,140 ind. The produced egg diameter (248-255 ¼m) and larva quality were relatively same between the two treatments. Amino acid profiles in the hepatopancreas and muscle were higher in the wild male broodstock compared to the cultivated (F-1) male broodstock. In conclusion, wild male spermatophore is generally better than the cultivated F-1 male spermatophore for artificial insemination of female broodstock F-1.

Application of dsRNA VP24 vaccine by oral administration at different larval stages of Tiger Shrimp Penaeus monodon

“…Application of dsRNA vaccines on mysis stages showed higher morphological values in the front intestine, tail fan, and tail muscles compared to treatments on stadia zoea, PL, and control (without dsRNA vaccine). In stages mysis, the digestive organs are already perfectly formed, so the feed given can be well digested [22]. The PL morphological values obtained in this study were higher than larvae of non transgenic (75) and transgenic (85) tiger shrimp [21] and relatively the same as the PL morphological values of shrimp spawning ablation spawning and induced maturation hormones without ablation 87 and 91 respectively [23], morphology value of black tiger shrimp larvae were using the different probiotic application which is ranging from (71-93) [24].…”