Pengembangan Model Mencit Alergi dengan Paparan Kronik Ovalbumin

Abstract

Histology expression in asthma show inflammatory processes and airway structural changes. Treatment development in asthma needs appropriate animal models but most animal models use short term allergen exposure. The aim of this research was to create develop allergic mouse model with allergic inflammation and structural changes histology expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 grams were divided into two groups. The first group were exposed to a normal saline solution where the second group received ovalbumin. Mice were sensitized by intraperitoneal injection of ovalbumin on day 0 and 14 and challenged by nebulization of 1% ovalbumin three times weekly for 6 weeks. Twenty four hours after final exposure, the mice were sacrificed by lethal cardiac puncture. The level of total IgE in serum were measured by ELISA and IL-4 receptor expression were examinated by immunohistochemistry. Morphometric analysis was performed to count inflammatory cells, eosinophil, goblet cells, epithel and smooth muscle thickness. Differences between the control and exposed mice were analyzed by Mann-Whitney test. There were a significant differences in total IgE serum and expression IL-4 receptor between the two groups (P < 0,05). The morphometric analyzing also demonstrated significant differences between these two groups (P < 0,05). Chronic ovalbumin exposure through inhalation produced allergic inflammation and airway structural changes.

The Decrease of Lung Eosinophil Following Zinc Supplementation in Mouse Models of Respiratory Allergic Inflammation

“…The sensitization of OVA in this study successfully triggered inflammations in the respiratory tract of the allergy animal models [9,11]. Sensitization OVA by intra-peritoneal injection on days 0 and 14, followed by inhalations of OVA for twenty minutes with a frequency of three times a week for six weeks providing the inflammatory responses with infiltration of inflammatory cells and eosinophil were significantly higher in the group of OVA than in the negative control group (p = 0.029 ) [12,13].…”

“…The selection of the female BALB/c mice strain in developing allergy mouse model was selected based on the higher degree of sensitivity in order to activate dominancy of Th2 lymphocytes compared to other strains. The OVA sensitization in the BALB/c mice, which results in a significant difference between the P levels of mucus secretion of goblet cells and levels of OVA-specific IgE antibodies, are higher than the OVA sensitization in mice C57BL [6,[11][12][13][14].…”

Pengaruh Paparan Akut Asam Sulfat (HSO) dan Asam Nitrat (HNO) terhadap Penebalan 2 4 3 Septum Interalveolaris Tikus

“…Sel goblet kecenderungan peningkatan kadar TGF-β yang merupakan mencit asma menunjukkan peningkatan jumlah maupun salah satu sitokin supresor yang dihasilkan oleh T reg. Studi ukuran (hiperplasia) dengan rerata jumlah sel goblet/100 lain menunjukan proses inflamasi dan airway remodelling µm membran basalis sebesar 1,44±0,26 dibandingkan tetap terjadi pada paparan kronik ovalbumin (17,22). Hal kontrol/mencit non asma sebesar 1,07±0,12.…”

“…Paparan ovalbumin selama 9 minggu dalam penelitian ini menyebabkan perubahan struktur saluran napas. Inhalasi ovabulmin kronik menginduksi inflamasi kronis yang berakibat terjadinya airway remodeling (17,22).…”

Profilin Menginduksi Ekspresi TLR-11, Il-6, dan TNF- sebagai Kandidat Prediktor Disfungsi Adiposit akibat Infeksi Toxoplasma Gondii α

“…adjuvant yang membuat efek alergen ovalbumin Pengetahuan tentang patologi, patofisiologi, dan bertambah kuat dan sensitisasi berlangsung lebih imunologi asma berkembang sangat pesat, tetapi lama (18,19,26,28). Sensitisasi awal dilakukan secara mekanisme dasar perkembangan penyakit ini masih intraperitoneal dengan menyuntikkan campuran 10 μg belum jelas.…”