Penggunaan Minyak Zaitun Ekstra Virgin ke dalam Bahan Pengencer Semen terhadap Kualitas Spermatozoa Ayam Lokal

Khaeruddin, Khaeruddin; Sumantri, Cece; Darwati, Sri; Arifiantini, Raden Iis

doi:10.29244/3.1.46-51

Cited by 4 publications

(7 citation statements)

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“…Saat ini sudah banyak digunakan pengencer seperti ringer laktat, NaCl fisiologis, dekstrosa dengan berbagai macam bahan additive seperti orange jus (Al-Daraji, 2012b), jus buah delima (Al-Daraji, 2015) dan jus tomat (Al-daraji, 2014). Al-Daraji (2012a) dan Khaeruddin et al (2015) juga telah memperkenalkan minyak zaitun, dan ekstrak bawang putih (Al-Daraji, 2013) sebagai bahan additive pengencer semen ayam. Al-Daraji (2012c) menjelaskan bahwa kandungan antioksidan dalam bahan additive tersebut mampu mempertahankan kualitas spermatozoa ayam yang mengandung asam lemak tak jenuh yang mudah rusak karena kontak langsung dengan udara ketika semen diejakulasikan.…”

Section: Pendahuluanunclassified

“…Minyak buah merah adalah produk ekstraksi buah merah (Pandanus conoideus Lam) yang menurut Budi dan Paimin (2004) mengandung cukup banyak antioksidan seperti, karotenoid (12.000ppm), tokoferol (11.000ppm) juga asam lemak tak jenuh seperti asam oleat, asam linoleat, asam linolenat dan asam dekanoat sehingga potensial untuk digunakan sebagai bahan additive pada pengencer semen ayam. Penggunaan minyak zaitun dan ekstrak bawang putih dapat meningkatkan motilitas massa, motilitas individu spermatozoa, viabilitas dan menurunkan abnormalitas spermatozoa (Al-Daraji, 2012a; Al-Daraji, 2013; Khaeruddin et al, 2015).…”

Section: Pendahuluanunclassified

“…Peroksidasi lipid dapat diatasi dengan cara melindungi membran sel spermatozoa dengan antioksidan (Wirahadikusumah, 1985;Al-Daraji, 2012a;Khaeruddin et al, 2015). Tokoferol merupakan antioksidan kuat yang secara umum mampu mencegah radikal bebas dan aktivasi endonuklease.…”

Section: Pendahuluanunclassified

“…Faktor lain yang mungkin mengakibatkan menurunnya motilitas spermatozoa ayam pada penelitian dengan menambahkan minyak buah merah ini adalah kandungan minyak yang cukup tinggi pada ekstrak buah merah yang mungkin ikut menghambat pergerakan spermatozoa. Menurut Khaeruddin et al (2015) angka motilitas spermatozoa yang diencerkan dengan tambahan (MZEV) yang lebih banyak akan berkurang sebab pergerakannya sangat dipengaruhi oleh banyaknya molekulmolekul MZEV yang menghambat pergerakannya. Hal yang sama dilaporkan Solihati (2008) bahwa pemberian santan sebagai pengencer semen dapat menghambat pergerakan spermatozoa karena santan mengandung lemak.…”

Section: Hasil Dan Pembahasanunclassified

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Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)

Isnaeni

Faidiban

Tethool³

2019

J. Il. Pet. Vet. Trop.

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Red fruit oil is a red fruit extraction product (Pandanus conoideus Lam) containing a lot of antioxidants such as carotenoids, tocopherols as well as unsaturated fatty acids such as oleic acid, linoleic acid, linolenic acid and decanoic acid which is potential to be used as an additive for semen chicken diluents. This study aims to obtain an information about the effectiveness of adding red fruit oil as a cement diluent derived from male native chickens. The treatment consisted of 0.9 ml ringer laktat + 0.1 ml semen (Pl), ringer laktat 0.98 ml + 0.1 ml semen + 0.02 ml red fruit oil (P2), ringer laktat 0.96 ml + 0.1 ml semen + 0.04 ml red fruit oil (P3), ringer laktat 0.94 ml + 0.1 ml cement + 0.06 ml red fruit oil (P4). The results showed the sperm motility ranged from 48.17 ± 7.86% to 84.67 ± 2.42%. Treatment P1 and P2 have a very significant difference (P <0.01) on motility 0.05). The spermatozoa concentration ranged from 1,600x106 cells / ml to 2,200x106 cells/ml. The spermatozoa concentration of treatment P1 and P2 chickens did not show any significant differences (P> 0.05), nor did among treatments P2, P3 and P4. However, treatment P1 has a significant difference on spermatozoa concentration with P3 and P4. (P <0.05). It can be concluded that the addition of red fruit oil in lactated ringer diluent can be used as an additive for semen diluents because it is able to maintain the concentration. In addition, adding of red fruit oil up to concentrations of more than 2% tend to reduce sperm motility. Keywords: Concentration, Motility, Spermatozoa and Red fruit oil

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Section: Pendahuluanunclassified

Section: Hasil Dan Pembahasanunclassified

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Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)

Isnaeni

Faidiban

Tethool³

2019

J. Il. Pet. Vet. Trop.

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“…Pengamatan viabilitas spermatozoa dilakukan dengan menggunakan pewarnaan eosin (Khaeruddin et al, 2015). Sampel semen dan pewarna eosin (1:3) dicampur pada object glass dan dibuat preparat ulas tipis pada object glass yang lain.…”

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Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung

Inggriani

Tethool²,

Lumatauw

2020

J. Il. Pet. Vet. Trop.

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Antioxidants can inhibit the oxidation process by reacting with free radicals, which in turn, maintain the quality of spermatozoa. This study aims to determine the viability and the abnormality of “ayam kampung” (Gallus gallus) spermatozoa after being diluted in ringer lactate infused with “sarang semut” (Myrmecodia Sp) extract. RAL is the research method used, utilizing 4 different Gallus gallus as test subjects. The variables observed are the abnormality and the viability of the spermatozoa sample treated with different amounts of Myrmecodia Sp extract, P0: 0 ml, P1: 0.02 ml, P2: 0.03, and P3: 0.04 ml. The abnormality rate for each sample is as follows; P0: 14 ± 4.05%, P1: 13.5 ± 3.78%, P2: 3.5 ± 1.23% and P3: 12.83 ± 5.04 %. Using the variance analysis, the result showed that the addition of Myrmecodia Sp extract in ringer lactate solution had little to no effect on causing spermatozoa abnormalities. Furthermore, the viability of spermatozoa for each sample is; P0: 14 ± 4.05%, P1: 13 ± 3.78%, P2: 13 ± 1.22% and P3: 12 ± 5.04%. Based on the variance analysis, the result showed that Myrmecodia Sp extract had a significant effect (P <0.05) on the spermatozoa viability. The addition of Myrmecodia sp extract to ringer lactate diluter had little to no effect on the abnormality of the Gallus gallus’ spermatozoa but had a significant effect on the spermatozoa’s viability.

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Cemani chicken: characteristics of fresh semen, testes morphometric, and the relationship between the two traits

Kostaman,

Azizah,

Adiati

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The monitoring of the reproductive performance of roosters can be conducted by examining the characteristics of fresh semen, the morphometric approach of the testes, and the correlation between these two traits. To analyze the semen traits, a total of ten rooster heads were utilized, while the morphometric analysis of the testes was performed on five rooster heads. Descriptive methods were employed to assess the shape and characteristics of the fresh semen. The average length, width, weight, and circumference of the testes were evaluated using a T-test with a confidence level of 95%. Furthermore, Pearson’s correlation was employed to investigate the association between the traits of the semen and the morphology of the testes. The effects confirmed that the testes of Cemani chickens had been no extraordinary from chickens in general, placed inside the frame hollow space close to the spine, connected to the dorsal belly hollow space, or simply in the back of the lungs and the front of the kidneys and testes, elongated and oval. Typically, the left testes were relatively longer, wider, and heavier than the right testes. The semen exhibited a creamy white color, with a thick consistency and an acidity level of seven. Furthermore, it displayed a satisfactory mass movement with an average value of 2.80 ± 0.92. The motility and viability of the spermatozoa were within the normal range (70.50 ± 5.50 and 80.90 ± 4.58, respectively) at a concentration of 1402 ± 382 x 106/ml. The characteristics of the semen and the size of the testes (both left and right) demonstrated a correlation with motility. This observation concluded was that Cemani chickens exhibit remarkably accurate semen characteristics and testicular morphometrics.

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Penggunaan Minyak Zaitun Ekstra Virgin ke dalam Bahan Pengencer Semen terhadap Kualitas Spermatozoa Ayam Lokal

Cited by 4 publications

References 0 publications

Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)

Konsentrasi dan Motilitas Spermatozoa Ayam Kampung (Gallus domesticus) dalam Pengencer Ringer Laktat Yang Diberi Tambahan Minyak Buah Merah (Pandanus conoideus Lam)

Pengaruh Ekstrak Sarang Semut (Myrmecodia Sp) dalam Pengencer Ringer Laktat Terhadap Abnormalitas dan Viabilitas Spermatozoa Ayam Kampung

Cemani chicken: characteristics of fresh semen, testes morphometric, and the relationship between the two traits

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