Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering

Marui, Junichiro; Ohashi-Kunihiro, Sumiko; Ando, Tomohiro; Nishimura, Mika; Koike, Hideaki; Machida, Masayuki

doi:10.1016/j.jbiosc.2010.01.001

Cited by 43 publications

(25 citation statements)

References 16 publications

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“…Recently, it was reported that deleting laeA diminishes kojic acid production and gene expression in A. oryzae (39). Additionally, penicillin production in A. oryzae is diminished after deletion of veA, which is a member of the LaeA complex (17,40). These reports suggest that the LaeA complex also plays a general role in the induction of the SM gene cluster in A. oryzae.…”

Section: Phylogeny and Morphology Of Aohdacsmentioning

confidence: 95%

Fungus-Specific Sirtuin HstD Coordinates Secondary Metabolism and Development through Control of LaeA

2013

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bThe sirtuins are members of the NAD ؉ -dependent histone deacetylase family that contribute to various cellular functions that affect aging, disease, and cancer development in metazoans. However, the physiological roles of the fungus-specific sirtuin family are still poorly understood. Here, we determined a novel function of the fungus-specific sirtuin HstD/Aspergillus oryzae Hst4 (AoHst4), which is a homolog of Hst4 in A. oryzae yeast. The deletion of all histone deacetylases in A. oryzae demonstrated that the fungus-specific sirtuin HstD/AoHst4 is required for the coordination of fungal development and secondary metabolite production. We also show that the expression of the laeA gene, which is the most studied fungus-specific coordinator for the regulation of secondary metabolism and fungal development, was induced in a ⌬hstD strain. Genetic interaction analysis of hstD/ Aohst4 and laeA clearly indicated that HstD/AoHst4 works upstream of LaeA to coordinate secondary metabolism and fungal development. The hstD/Aohst4 and laeA genes are fungus specific but conserved in the vast family of filamentous fungi. Thus, we conclude that the fungus-specific sirtuin HstD/AoHst4 coordinates fungal development and secondary metabolism via the regulation of LaeA in filamentous fungi.

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Section: Phylogeny and Morphology Of Aohdacsmentioning

confidence: 95%

Fungus-Specific Sirtuin HstD Coordinates Secondary Metabolism and Development through Control of LaeA

2013

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“…In Aspergillus flavus , VeA and VelB are involved in the regulation of conidial production and sclerotia formation [17]–[19]. VeA also regulates the biosynthesis of secondary metabolites, including aflatoxin in A. flavus [20] and A. parasiticus [21], gliotoxin in A. fumigatus [16], and penicillin in A. oryzae [22].…”

Section: Introductionmentioning

confidence: 99%

VelC Positively Controls Sexual Development in Aspergillus nidulans

et al. 2014

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Fungal development and secondary metabolism is intimately associated via activities of the fungi-specific velvet family proteins including VeA, VosA, VelB and VelC. Among these, VelC has not been characterized in Aspergillus nidulans. In this study, we characterize the role of VelC in asexual and sexual development in A. nidulans. The velC mRNA specifically accumulates during the early phase of sexual development. The deletion of velC leads to increased number of conidia and reduced production of sexual fruiting bodies (cleistothecia). In the velC deletion mutant, mRNA levels of the brlA, abaA, wetA and vosA genes that control sequential activation of asexual sporulation increase. Overexpression of velC causes increased formation of cleistothecia. These results suggest that VelC functions as a positive regulator of sexual development. VelC is one of the five proteins that physically interact with VosA in yeast two-hybrid and GST pull down analyses. The ΔvelC ΔvosA double mutant produced fewer cleistothecia and behaved similar to the ΔvosA mutant, suggesting that VosA is epistatic to VelC in sexual development, and that VelC might mediate control of sex through interacting with VosA at specific life stages for sexual fruiting.

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“…To increase the production of biologically active secondary metabolites, several approaches in genetic engineering are frequently used. They include, for example: introduction of additional copies of genes coding for the specific biosynthetic pathway enzymes, an increase in the expression of these genes, and an increase in the number of genes encoding resistance to secondary metabolites . In this respect, genes exhibiting positive pleiotropic regulatory effects will be particularly important.…”

Section: Introductionmentioning

confidence: 99%

Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress

2017

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Improvement of the biosynthetic capabilities of industrially relevant microbes to produce desired metabolites in higher quantities is one of the important topics of modern biotechnology. In this article, different strategies of improvement of mutated microbial strains are briefly described. This is followed by the first comprehensive review of the literature on obtaining high yielding microorganisms, that is, mutants exhibiting resistance to antimetabolites, nutritional repression, and abiotic stresses as well as tolerance to solvents and toxic substrates or products. Furthermore, the efficiency of the microbial metabolites produced by improved microbial strains, advantages, and limitations, as well as future prospects for strategies of strain development are discussed.

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Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering

Cited by 43 publications

References 16 publications

Fungus-Specific Sirtuin HstD Coordinates Secondary Metabolism and Development through Control of LaeA

Fungus-Specific Sirtuin HstD Coordinates Secondary Metabolism and Development through Control of LaeA

VelC Positively Controls Sexual Development in Aspergillus nidulans

Strain improvement of industrially important microorganisms based on resistance to toxic metabolites and abiotic stress

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