Penicillin impregnation on oxygen plasma surface functionalized chitosan/ Antheraea assama silk fibroin: Studies of antibacterial activity and antithrombogenic property

Choudhury, Arup; Gogoi, Dolly; Kandimalla, Raghuram; Kalita, Sanjeeb; Chaudhari, Yogesh B.; Khan, Mojibur R.; Kotoky, Jibon; Chutia, Joyanti

doi:10.1016/j.msec.2015.11.070

Cited by 29 publications

(21 citation statements)

References 23 publications

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“…The antifungal assay was carried out by agar well diffusion method ( Bora, 2016 ; Choudhury et al, 2016a , b ). EO-CN was reconstituted in dimethyl sulfoxide (DMSO) at different concentrations (20–100 μg/ml) to evaluate the anti- Candida activity.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

et al. 2016

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Poor wound healing is one of the major complication of diabetic patients which arises due to different factors like hyperglycemia, oxidative stress, vascular insufficiency and microbial infections. Candidiasis of diabetic wounds is a difficult to treat condition and potentially can lead to organ amputation. There are a few number of medications available in market to treat this chronic condition; which demands for alternative treatment options. In traditional system of medicine like Ayurveda, essential oil extracted from leaves of Cymbopogon nardus L. (Poaceae) has been using for the treatment of microbial infections, inflammation and pain. In this regard, we have evaluated anti-Candida and anti-inflammatory activity mediated wound healing property of C. nardus essential oil (EO-CN) on candidiasis of diabetic wounds. EO-CN was obtained through hydro-distillation and subjected to Gas chromatography–mass spectroscopy (GC–MS) analysis for chemical profiling. Anti-Candida activity of EO-CN was tested against Candida albicans, C. glabrata and C. tropicalis by in vitro zone of inhibition and minimum inhibitory concentration (MIC) assays. Anti-candidiasis ability of EO-CN was evaluated on C. albicans infected diabetic wounds of mice through measuring candida load on the 7th, 14th, and 21st day of treatment. Further progression in wound healing was confirmed by measuring the inflammatory marker levels and histopathology of wounded tissues on last day of EO-CN treatment. A total of 95 compounds were identified through GC–MS analysis, with major compounds like citral, 2,6-octadienal-, 3,7-dimethyl-, geranyl acetate, citronellal, geraniol, and citronellol. In vitro test results demonstrated strong anti-Candida activity of EO-CN with a MIC value of 25 μg/ml against C. albicans, 50 μg/ml against C. glabrata and C. tropicalis. EO-CN treatment resulted in significant reduction of candida load on diabetic wounds. Acceleration in wound healing was indicated by declined levels of inflammatory cytokines at wounded area in EO-CN treated animals compared to non-treated group, which was further confirmed by histopathological examination. This study suggests that through significant anti-Candida and anti-inflammatory activity, EO-CN attenuates the growth of the fungus on diabetic wounds and simultaneously reduces the inflammation which leads to acceleration of the wound healing process.

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Section: Methodsmentioning

confidence: 99%

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

et al. 2016

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“…In this study, the prepared nanohybrids (GSGNPs) were found to be biocompatible, whereas at higher concentration, very mild hemolysis was observed, which was in the permeable limit (5%). 32 Further, FE-SEM analysis confirmed that RBCs maintain their membrane integrity even after treatment with a high concentration (1 mg/mL) of GSGNPs for 24 h. Treatment of SGNPs and GSGNPs did not induce any toxic effect toward the L929 cells at different concentrations (5-250 µg/mL). The results of this study demonstrated that both SGNPs and GSGNPs do not possess any toxic chemical groups to cause damage to the mammalian cells.…”

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confidence: 63%

Glycogen–gold nanohybrid escalates the potency of silymarin

Kandimalla¹,

Dash²,

Bhowal³

et al. 2017

IJN

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In this study, a glycogen–gold nanohybrid was fabricated to enhance the potency of a promising hepatoprotective agent silymarin (Sly) by improving its solubility and gut permeation. By utilizing a facile green chemistry approach, biogenic gold nanoparticles were synthesized from Annona reticulata leaf phytoconstituents in combination with Sly (SGNPs). Further, the SGNPs were aggregated in glycogen biopolymer to yield the therapeutic nanohybrids (GSGNPs). Transmission electron microscopy, UV–Vis spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy analysis confirmed the successful formation and conjugation of both SGNPs and GSGNPs. The fabricated nanohybrids showed significant protection against CCl 4 -induced hepatic injury in Wistar rats and maintained natural antioxidant (superoxide dismutase and catalase) levels. Animals treated with GSGNPs (10 mg/kg) and SGNPs (20 mg/kg) retained usual hepatic functions with routine levels of hepatobiliary enzymes (aspartate transferase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase) and inflammatory markers (interleukin-1β and tumor necrosis factor-α) with minimal lipid peroxidation, whereas those treated with 100 mg/kg of Sly showed the similar effect. These results were also supported by histopathology of the livers where pronounced hepatoprotection with normal hepatic physiology and negligible inflammatory infiltrate were observed. Significant higher plasma C max supported the enhanced bioavailability of Sly upon GSGNPs treatment compared to SGNPs and free Sly. Graphite furnace atomic absorption spectrophotometry analysis also substantiated the efficient delivery of GSGNPs over SGNPs. The fabricated therapeutic nanohybrids were also found to be biocompatible toward human erythrocytes and L929 mouse fibroblast cells. Overall, due to increased solubility, bioavailability and profuse gut absorption; GSGNPs demonstrated tenfold enhanced potency compared to free Sly.

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“…This conclusion can be inferred from the appearance of a peak at 1644 cm −1 , related to the amide group formed by the reaction between penicillin G and amino groups on the γ-Fe2O3@SiO2 surface. In addition, the band associated with carbonyl group in the lactam ring of pure penicillin (1174 cm −1 ) [35] was absent in Prn/Fe2O3@SiO2 spectrum, pointing to ring opening upon functionalization. Furthermore, a strong band at 1080 cm −1 was observed for both γ-Fe2O3@SiO2 and Prn/Fe2O3@SiO2 materials, corresponding to Si-O-Si stretching vibration.…”

Section: Resultsmentioning

confidence: 98%

Unprecedented Proline-Based Heterogeneous Organocatalyst for Selective Production of Vanillin

et al. 2018

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An organocatalytic system based on an unprecedented proline analogue and iron oxide magnetic nanoparticles (Prn/Fe 2 O 3 @SiO 2) was designed and employed in vanillin production from isoeugenol and vanillyl alcohol. Full characterization of the obtained catalyst revealed the successful functionalization of the nanoparticle surface with the organic moieties. The activity of the magnetic bifunctional material was compared with its proton-unexchanged counterpart. Interestingly, the oxidation of isoeugenol resulted in being highly dependent on the acidic functionalities of the organocatalyst. Nonetheless, the catalytic performance of the proton-unexchanged catalyst suggested that the acidic and basic sites of the Prn/Fe 2 O 3 @SiO 2 exhibited a synergic effect, giving rise to higher conversion and selectivity. The presence of bifunctional groups in the proline analogue, together with the magnetic properties of the iron oxide nanoparticles, could lead to high efficiency, versatility, recoverability, and reusability.

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Penicillin impregnation on oxygen plasma surface functionalized chitosan/ Antheraea assama silk fibroin: Studies of antibacterial activity and antithrombogenic property

Cited by 29 publications

References 23 publications

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

Glycogen–gold nanohybrid escalates the potency of silymarin

Unprecedented Proline-Based Heterogeneous Organocatalyst for Selective Production of Vanillin

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Penicillin impregnation on oxygen plasma surface functionalized chitosan/ Antheraea assama silk fibroin: Studies of antibacterial activity and antithrombogenic property

Cited by 29 publications

References 23 publications

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

Chemical Composition and Anti-Candidiasis Mediated Wound Healing Property of Cymbopogon nardus Essential Oil on Chronic Diabetic Wounds

Glycogen&ndash;gold nanohybrid escalates the potency of silymarin

Unprecedented Proline-Based Heterogeneous Organocatalyst for Selective Production of Vanillin

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Glycogen–gold nanohybrid escalates the potency of silymarin