Penicillium subrubescens is a promising alternative for Aspergillus niger in enzymatic plant biomass saccharification

Mäkelä, Miia; Mansouri, Sadegh; Wiebenga, Ad; Rytioja, Johanna; Vries, Ronald P. de; Hildén, Kristiina

doi:10.1016/j.nbt.2016.07.014

Cited by 31 publications

(25 citation statements)

References 34 publications

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“…The levels of monomeric sugars and maltose in the culture liquid supernatants were determined by using high performance anion-exchange chromatography (HPAEC, Thermo) as previously described 33 .…”

Section: Methodsmentioning

confidence: 99%

The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

Mäkelä

Pontes

Rossen-Uffink

et al. 2018

Sci Rep

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In nature, the fungus Aspergillus niger degrades plant biomass polysaccharides to monomeric sugars, transports them into its cells, and uses catabolic pathways to convert them into biochemical building blocks and energy. We show that when grown in liquid cultures, A. niger takes up plant-biomass derived sugars in a largely sequential manner. Interestingly, this sequential uptake was not mediated by the fungal general carbon catabolite repressor protein CreA. Furthermore, transcriptome analysis strongly indicated that the preferential use of the monomeric sugars is arranged at the level of transport, but it is not reflected in transcriptional regulation of sugar catabolism. Therefore, the results indicate that the regulation of sugar transport and catabolism are separate processes in A. niger.

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Section: Methodsmentioning

confidence: 99%

The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

Mäkelä

Pontes

Rossen-Uffink

et al. 2018

Sci Rep

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“…The plate was then centrifuged (10 min, 3000 × g ), the supernatant was diluted 5-fold in water and analyzed. The enzyme products were identified by High Performance Liquid Chromatography (HPLC) as described previously 45 . Statistical significance was tested using Student’s t -test.…”

Section: Methodsmentioning

confidence: 99%

Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin

Kowalczyk

Lubbers

Peng

et al. 2017

Sci Rep

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Aspergillus niger produces an arsenal of extracellular enzymes that allow synergistic degradation of plant biomass found in its environment. Pectin is a heteropolymer abundantly present in the primary cell wall of plants. The complex structure of pectin requires multiple enzymes to act together. Production of pectinolytic enzymes in A. niger is highly regulated, which allows flexible and efficient capture of nutrients. So far, three transcriptional activators have been linked to regulation of pectin degradation in A. niger. The L-rhamnose-responsive regulator RhaR controls the production of enzymes that degrade rhamnogalacturonan-I. The L-arabinose-responsive regulator AraR controls the production of enzymes that decompose the arabinan and arabinogalactan side chains of rhamnogalacturonan-II. The D-galacturonic acid-responsive regulator GaaR controls the production of enzymes that act on the polygalacturonic acid backbone of pectin. This project aims to better understand how RhaR, AraR and GaaR co-regulate pectin degradation. For that reason, we constructed single, double and triple disruptant strains of these regulators and analyzed their growth phenotype and pectinolytic gene expression in A. niger grown on sugar beet pectin.

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“…Enzymes present in the supernatants collected from the compartments were heat inactivated at 99 C for 5 min before sugar analysis of the liquids to avoid further conversion of the sugars. L-arabinose, D-glucose, D-xylose and D-galacturonic acid were measured using a Dionex HPAEC-PAD (Dionex ICS-5000+ system; Thermo Scientific) system with the program for measuring the monosaccharides as described previously (Mäkelä et al, 2016).…”

Section: Sugar Analysis Of Compartments After Culturingmentioning

confidence: 99%

Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Daly

Peng

Mitchell

et al. 2020

Environmental Microbiology

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Summary Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network of branching and fusing hyphae that are often considered to be relatively uniform entities in which nutrients can freely move through the hyphae. In nature, different parts of a colony are often exposed to different nutrients. We have investigated, using a multi‐omics approach, adaptation of A. niger colonies to spatially separated and compositionally different plant biomass substrates. This demonstrated a high level of intra‐colony differentiation, which closely matched the locally available substrate. The part of the colony exposed to pectin‐rich sugar beet pulp and to xylan‐rich wheat bran showed high pectinolytic and high xylanolytic transcript and protein levels respectively. This study therefore exemplifies the high ability of fungal colonies to differentiate and adapt to local conditions, ensuring efficient use of the available nutrients, rather than maintaining a uniform physiology throughout the colony.

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Penicillium subrubescens is a promising alternative for Aspergillus niger in enzymatic plant biomass saccharification

Cited by 31 publications

References 34 publications

The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin

Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

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