Peningkatan Penggunaan Antibiotik Bijak Melalui Deteksi Dini Mrsa Pada Posdaya Sunan Kalijaga,  Berkoh, Purwokerto

Anjarwati, Dwi Utami; Sari, Rahmawati Wulan; Hestiyani, Rani Afifah Nur

doi:10.20884/1.dj.2020.2.1.962

Cited by 1 publication

(2 citation statements)

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“…This is because biofilms contain extracellular polymeric substances (EPS) that envelop or wrap a collection of microorganisms that gather into colonies, making it difficult for antibiotics to penetrate the EPS to attack these microorganisms [ 7 ]. In addition, phenotypic and genotypic changes in gene expression are another cause of antibiotic resistance in biofilms [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Docking Study of the C-10 Massoia Lactone Compound as an Antimicrobial and Antibiofilm Agent against Candida tropicalis

Hamzah,

Nuryastuti,

Rahmah

et al. 2023

The Scientific World Journal

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Antimicrobial resistance is now considered a global health problem because it reduces the effectiveness of antimicrobial drugs. According to the World Health Organization (WHO), the highest mortality rate is associated with infections caused by multidrug-resistant microorganisms, with approximately 700,000 deaths worldwide each year. The aim of this study was to determine the potential of C-10 massoia lactone to inhibit the growth of fungi and C. tropicalis biofilm, and molecular docking studies were performed to determine the nature of the inhibition. The study was conducted using the microdilution method for antifungal and antibiofilm testing and designed with a molecular docking approach. Furthermore, an analysis using the scanning electron microscope (SEM) was performed to evaluate the mechanism of effect. The results obtained showed that C-10 massoia lactone can inhibit the growth of fungi by 84.21% w/v. Meanwhile, the growth of C. tropicalis biofilm in the intermediate phase was 80.23% w/v and in the mature phase was 74.23% w/v. SEM results showed that C-10 massoia lactone damaged the EPS matrix of C. tropicalis so that hyphal formation was hindered due to damage to fungal cells, resulting in a decrease in attachment, density, and lysis of C. tropicalis fungal cells. Based on molecular docking tests, C-10 massoia lactone was able to inhibit biofilm formation without affecting microbial growth, while docking C-10 massoia lactone showed a significant binding and has the potential as an antifungal agent. In conclusion, the C-10 massoia lactone compound has the potential as an antibiofilm against C. tropicalis, so it can become a new antibiofilm agent.

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Section: Introductionmentioning

confidence: 99%

“…The management of infections caused by biofilms is very important because infections caused by biofilms can affect the morbidity and mortality of a person and can cause death if not properly treated [ 8 , 12 ]. It is known that biofilm infections cause the death of 7500 people per year [ 13 ].…”

Section: Introductionmentioning

confidence: 99%