No abstract

IMPROVED USE OF ANTIBIOTIC BY USING TRANSFER OF KNOWLEDGE INTERVENTIONS TO CHANGE THE COMMUNITY PERCEPTION IN BANYUMAS DISTRICT.The use of antibiotics without indication by the medical community and the community became the trigger factor of increasing the incidence of antibiotic resistance in Indonesia. Meanwhile by 20150 antibiotic resistance is predictedto be the leading cause of death in the world. Community devotion was aimed at improving the understanding of society so that that is a change of perception about the use of antibiotic wisely. The target respondents of devotion consists of member of the family welfare empowerment group (PKK). A seriaes of activities undertaken were pre and post test to measure respondents' understanding of antibiotic use before and after intervention in the transfer of knowledge. The questionnaire used previously tested its validity by Pearson product moment test and its reliability by Alpha-Cronbach. The Sharpening of material obtained in knowledge transfer was done with role play and discussion. The result of T test analysis to score of quesionnaire before and after knowledge transfer showed significant p value (p = 0,049). The conclusion was that the transfer of knowledge can change the perception of PKK members about the wise use of antibiotics.

Penggunaan antibiotik yang tidak bijak dapat menyebabkan peningkatan kejadian resistensi antibiotik di masyarakat. Pengabdian masyarakat ini bertujuan untuk melakukan deteksi dini (skrining) MRSA sebagai salah satu upaya untuk meningkatkan pengetahuan dan membangun kesadaran masyarakat dalam penggunaan antibiotik secara bijak. Metode yang digunakan dalam pengabdian masyarakat ini berupa identifikasi MRSA dari hapusan hidung dan tenggorok anggota posdaya Sunan Kalijaga, Berkoh, Purwokerto. Test kepekaan antibiotik yang dilakukan adalah dengan menggunakan metode difusi cakram, Kirby Bauer. Riwayat penggunaan antibiotik pada masyarakat diketahui dengan menggunakan kuesioner. Hasil kegiatan ini menunjukkan bahwa bakteri S.aureus yang ditemukan dalam masyarakat masih bersifat sensitif terhadap sefoxitin. Proporsi Stapylococcus aureus yang diperoleh sebanyak 9,33% dari hapusan hidung dan 34,66% dari hapusan tenggorok. Namun penggunaan antibiotik yang tidak bijak masih ditemukan pada anggota posdaya sebanyak 17.33%. Hasil kegiatan diinformasikan kepada masyarakat sebagai bahan acuan untuk memperbaiki perilaku penggunaan antibiotik secara bijak.

Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion using MTT assay. Whereas planktonic susceptibility testing was carried out using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.

Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen. Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia.Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district.  Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method.Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination,  one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene.Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.