Pentachlorophenol Dechlorination in Fluidized-Bed Reactors under Methanogenic Conditions

Zou, Siwei; Anders, Krista M.; Ferguson, John F.

doi:10.1080/10889869991219244

Cited by 6 publications

(2 citation statements)

References 36 publications

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“…For technical purposes, i.e. large-scale bioremediation of contaminated aquifers and waste water streams, the application of these complex consortia may represent a promising technology as shown by several experiments using laboratory-scale bioreactors (Hauck et al, 1999 ;Zou et al, 1999). However, due to the unknown community structure these consortia have been treated as ' black boxes ' in the past and a direct optimization of microbial biodegradation has been hampered.…”

Section: Discussionmentioning

confidence: 99%

Bacteria of an anaerobic 1,2-dichloropropane-dechlorinating mixed culture are phylogenetically related to those of other anaerobic dechlorinating consortia.

Schlötelburg¹,

Wintzingerode²,

Hauck³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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A 16S-rDNA-based molecular study was performed to determine the bacterial diversity of an anaerobic, 1,2-dichloropropane-dechlorinating bioreactor consortium derived from sediment of the River Saale, Germany. Total community DNA was extracted and bacterial 16S rRNA genes were subsequently amplified using conserved primers. A clone library was constructed and analysed by sequencing the 16S rDNA inserts of randomly chosen clones followed by dot blot hybridization with labelled polynucleotide probes. The phylogenetic analysis revealed significant sequence similarities of several as yet uncultured bacterial species in the bioreactor to those found in other reductively dechlorinating freshwater consortia. In contrast, no close relationship was obtained with as yet uncultured bacteria found in reductively dechlorinating consortia derived from marine habitats. One rDNA clone showed S97 % sequence similarity to Dehalobacter species, known for reductive dechlorination of tri-and tetrachloroethene. These results suggest that reductive dechlorination in microbial freshwater habitats depends upon a specific bacterial community structure.

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Section: Discussionmentioning

confidence: 99%

Bacteria of an anaerobic 1,2-dichloropropane-dechlorinating mixed culture are phylogenetically related to those of other anaerobic dechlorinating consortia.

Schlötelburg¹,

Wintzingerode²,

Hauck³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…Microorganisms were immobilized on polyurethane foam particles and fluidized within the reactor by continuous flow‐through of DCP‐containing medium. As deduced from pilot experiments using laboratory‐scale bioreactors, application of microbial mixed cultures for large‐scale bioremediation of contaminated aquifers and waste water streams may represents a promising bioremediation technology [2–4]. However, one major drawback of this technology is the unknown composition of the dechlorinating microbial community, hindering the optimization and efficient control of the transformation processes.…”

Section: Introductionmentioning

confidence: 99%

Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropane

Schlötelburg

Wintzingerode²,

Hauck

et al. 2002

FEMS Microbiology Ecology

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The bacterial diversity of an anaerobic 1,2-dichloropropane (DCP) dechlorinating bioreactor consortium derived from river sediment has been investigated by a combined molecular approach. By using rDNA clone libraries, denaturing gradient gel electrophoresis and quantitative real-time PCR, both Dehalococcoides ethenogenes- and Dehalobacter restrictus-like 16S rDNA sequences were found within the community. Both species are known for reductive dechlorination of tetrachloroethene. Furthermore, numerous yet-uncultured members of the Green non-sulfur bacteria occurred within the consortium. The community analyses over a period of 14 months revealed a clear population shift. D. restrictus 16S rDNA was enriched significantly and became the most abundant rDNA sequence type, suggesting that Dehalobacter spp. play a key role within the reductive dechlorination of DCP in this consortium. We propose the use of this species as an indicator to monitor the transformation process within the bioreactor.

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Toxic effects of thiol-reactive compounds on anaerobic biomass

Tepe¹,

Shimko²,

Duran³

2006

Bioresource Technology

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Pentachlorophenol Dechlorination in Fluidized-Bed Reactors under Methanogenic Conditions

Cited by 6 publications

References 36 publications

Bacteria of an anaerobic 1,2-dichloropropane-dechlorinating mixed culture are phylogenetically related to those of other anaerobic dechlorinating consortia.

Bacteria of an anaerobic 1,2-dichloropropane-dechlorinating mixed culture are phylogenetically related to those of other anaerobic dechlorinating consortia.

Microbial structure of an anaerobic bioreactor population that continuously dechlorinates 1,2-dichloropropane

Toxic effects of thiol-reactive compounds on anaerobic biomass

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