Penyimpanan Bakteri Escherichia coli Dan Streptococcus pneumonie Pada Media Cryoprotective Dengan Metode Freeze Drying

Abstract

Proses perbanyakan dan penyimpanan bakteri menjadi hal yang harus diperhatikan karena cara penyimpanan sangat terkait dengan perubahan sifat bakteri yang disimpan. Di Laboratorium, kultur bakteri murni dipindahkan dengan metode subkultur. Kekurangan subkultur� berulang adalah memakan waktu, beresiko kontaminasi dan mutasi genetik. Penelitian ini bertujuan untuk mengembangkan penyimpanan bakteri jangka panjang yang aplikatif di Laboratorium Mikrobiologi serta menentukan jenis Cryoprotectan Agent yang dapat mempertahankan viabilitas sel Escherichia coli dan Streptococcus pneumonie dengan metode Freeze Drying. Metode penelitian yang digunakan adalah eksperimen dengan menambahkan Skim Milk 10% dan gliserol 10% sebagai Cryoprotectan Agent pada pelet sel Escherichia coli dan Streptococcus pneumonie dengan metode Freeze Drying. Digunakan kontrol tanpa penambahan Cryoprotectan Agent dengan metode Freeze Drying dan kontrol tanpa penambahan Cryoprotectan Agent� yang disimpan dalam refrigator (non Freeze Drying) kemudian jumlah koloni bakteri dihitung setelah masa simpan 2,4,6 minggu.Selanjutnya data yang diperoleh diolah secara statistik menggunakan metode statistik non parametrik kruskal wallis . Hasil penelitian menunjukkan bahwa Skim Milk 10% mampu mempertahankan viabilitas Sel Escherichia coli dengan persentase penurunan viabilitas paling rendah yaitu 4,2%, namun� jenis Cryoprotectan agent metode Freeze Dry belum mampu mengawetkan dan mempertahankan viabilitas sel Streptococcus pneumonie.Kata Kunci: Cryoprotectan Agent; Cell viability; Escherichia coli;, Streptococcus pneumonie; Freeze ��Drying

Contamination of Escherichia Coli Bacteria in School Children's Food (Pjas) in Cakung District Elementary School

“…Based on the results of statistical tests, it was obtained that the P-value was 1.00, which means that at 5% there was no significant relationship between the presence of sanitation facilities and E.coli contamination in PJAS in SD Cakung District. 6 it is known that 59% of the traders who have food storage facilities that do not meet the requirements are positive for containing E.coli, while 19% of the traders who have food storage facilities that meet the requirements are positive for positive E.coli. Based on the results of statistical tests, a P-value of 0.007 was obtained, which means that at 5% there was a significant relationship between places to store cooked food and E.coli contamination in PJAS at SD Cakung District in 2016.…”

“…or semi-official and carried out in the morning, afternoon, evening, or evening [5]. Based on the type of Food Management Place (TPM), E.coli contamination in food is most commonly found in street vendors as much as 40.7%, while other places include catering services 38.2%, hotels 33.3%, stalls 32.9 %, restaurants 31.3%, and food industry 21.3% [6].…”