Peptide Antibiotics Produced by Bacillus Species: First Line of Attack in the Biocontrol of Plant Diseases

Khatoon, Zobia; Orozco-Mosqueda, Ma. del Carmen; Huang, Suiliang; Nascimento, Francisco X.

doi:10.1007/978-3-030-85465-2_2

Cited by 5 publications

(3 citation statements)

References 85 publications

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“…In our results, we found that this genus dominated several BGC types such as RiPP‐like and lassopeptide, and has the potential to produce SMs, such as mycosubtilin, alnumycin, and paeninodin. Our results suggest that, just like Bacillus , the genus Siminovitchia can produce SMs with surfactant and antimicrobial activities (Kaspar et al, 2019 ; Khatoon et al, 2022 ). Within the Actinobacteria, our enrichment methods only recovered MAGs from the genus Rhodococcus ; which had a great number of NRPSs, as do previously described genomes of Rhodococcus strains isolated from several environments where NRPS, PKs, terpenes, and butyolactone have been reported as the dominant types of SMs (Ceniceros et al, 2017 ; Doroghazi & Metcalf, 2013 ).…”

Section: Discussionmentioning

confidence: 65%

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Suárez‐Moo,

Prieto‐Davó

2024

MicrobiologyOpen

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Microbial communities from various environments have been studied in the quest for new natural products with a broad range of applications in medicine and biotechnology. We employed an enrichment method and genome mining tools to examine the biosynthetic potential of microbial communities in the sediments of a coastal sinkhole within the karst ecosystem of the Yucatán Peninsula, Mexico. Our investigation led to the detection of 203 biosynthetic gene clusters (BGCs) and 55 secondary metabolites (SMs) within 35 high‐quality metagenome‐assembled genomes (MAGs) derived from these subcommunities. The most abundant types of BGCs were Terpene, Nonribosomal peptide‐synthetase, and Type III polyketide synthase. Some of the in silico identified BGCs and SMs have been previously reported to exhibit biological activities against pathogenic bacteria and fungi. Others could play significant roles in the sinkhole ecosystem, such as iron solubilization and osmotic stress protection. Interestingly, 75% of the BGCs showed no sequence homology with bacterial BGCs previously reported in the MiBIG database. This suggests that the microbial communities in this environment could be an untapped source of genes encoding novel specialized compounds. The majority of the BGCs were identified in pathways found in the genus Virgibacillus, followed by Sporosarcina, Siminovitchia, Rhodococcus, and Halomonas. The latter, along with Paraclostridium and Lysinibacillus, had the highest number of identified BGC types. This study offers fresh insights into the potential ecological role of SMs from sediment microbial communities in an unexplored environment, underscoring their value as a source of novel natural products.

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Section: Discussionmentioning

confidence: 65%

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Suárez‐Moo,

Prieto‐Davó

2024

MicrobiologyOpen

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“…In addition, there are reports that B. pumilus strains produce other antibiotic substances including bacilysin (dipeptide antibiotic), tetaine (dipeptide antibiotic), bacircine, amino sugar NTD, bacteriocin (antimicrobial peptide), phenazine (heterocyclic antibiotic), amicoumacin A, paenilamicin, and subtilin, which also exhibit antibacterial and antifungal properties ( Sansinenea and Ortiz, 2011 ; Özcengiz and Ögulur, 2015 ; Padaria et al, 2016 ; Toymentseva et al, 2019 ; Maksimova et al, 2021 ; Islam et al, 2022 ; Khatoon et al, 2022 ).…”

Section: Lipopeptides Synthesized By Bacillus Pumilusmentioning

confidence: 99%

Biocontrol of fungal phytopathogens by Bacillus pumilus

Dobrzyński¹,

Jakubowska²,

Kulkova³

et al. 2023

Front. Microbiol.

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Plant growth-promoting bacteria are one of the most interesting methods of controlling fungal phytopathogens. These bacteria can participate in biocontrol via a variety of mechanisms including lipopeptide production, hydrolytic enzymes (e.g., chitinase, cellulases, glucanase) production, microbial volatile organic compounds (mVOCs) production, and induced systemic resistance (ISR) triggering. Among the bacterial genera most frequently studied in this aspect are Bacillus spp. including Bacillus pumilus. Due to the range of biocontrol traits, B. pumilus is one of the most interesting members of Bacillus spp. that can be used in the biocontrol of fungal phytopathogens. So far, a number of B. pumilus strains that exhibit biocontrol properties against fungal phytopathogens have been described, e.g., B. pumilus HR10, PTB180, B. pumilus SS-10.7, B. pumilus MCB-7, B. pumilus INR7, B. pumilus SE52, SE34, SE49, B. pumilus RST25, B. pumilus JK-SX001, and B. pumilus KUDC1732. B. pumilus strains are capable of suppressing phytopathogens such as Arthrobotrys conoides, Fusarium solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Rhizoctonia solani, and Fagopyrum esculentum. Importantly, B. pumilus can promote plant growth regardless of whether it alters the native microbiota or not. However, in order to increase its efficacy, research is still needed to clarify the relationship between the native microbiota and B. pumilus. Despite that, it can already be concluded that B. pumilus strains are good candidates to be environmentally friendly and commercially effective biocontrol agents.

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“…Antimicrobial peptides or VOCs such as aliphatic ketones and sulfur compounds are able to induce plant defense responses and/or directly antagonize fungal pathogens ( Guevara-Avendaño et al, 2019 ; Nazari and Smith, 2020 ; Praveena et al, 2022 ). In this context, PGPR belonging to the Bacillus genus are particularly interesting, due to their ubiquity, ability to form endospores and tolerate harsh environmental conditions, their capacity to colonize roots, promote plant growth and development, induce systemic resistance in their hosts, and produce a wide range of specialized metabolites with antimicrobial activities ( Dimkić et al, 2017 ; Guevara-Avendaño et al, 2018 ; Khatoon et al, 2022 ; Luo et al, 2022 ). Such metabolites include diffusible compounds such as cyclic lipopeptides or polyketides, which have been largely described for their antimicrobial activity against a vast array of plant pathogens ( Gong et al, 2015 ; Caulier et al, 2019 ; Guevara-Avendaño et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol and plant growth promoting traits of two avocado rhizobacteria are orchestrated by the emission of diffusible and volatile compounds

et al. 2023

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Avocado (Persea americana Mill.) is a tree crop of great social and economic importance. However, the crop productivity is hindered by fast-spreading diseases, which calls for the search of new biocontrol alternatives to mitigate the impact of avocado phytopathogens. Our objectives were to evaluate the antimicrobial activity of diffusible and volatile organic compounds (VOCs) produced by two avocado rhizobacteria (Bacillus A8a and HA) against phytopathogens Fusarium solani, Fusarium kuroshium, and Phytophthora cinnamomi, and assess their plant growth promoting effect in Arabidopsis thaliana. We found that, in vitro, VOCs emitted by both bacterial strains inhibited mycelial growth of the tested pathogens by at least 20%. Identification of bacterial VOCs by gas chromatography coupled to mass spectrometry (GC–MS) showed a predominance of ketones, alcohols and nitrogenous compounds, previously reported for their antimicrobial activity. Bacterial organic extracts obtained with ethyl acetate significantly reduced mycelial growth of F. solani, F. kuroshium, and P. cinnamomi, the highest inhibition being displayed by those from strain A8a (32, 77, and 100% inhibition, respectively). Tentative identifications carried out by liquid chromatography coupled to accurate mass spectrometry of diffusible metabolites in the bacterial extracts, evidenced the presence of some polyketides such as macrolactins and difficidin, hybrid peptides including bacillaene, and non-ribosomal peptides such as bacilysin, which have also been described in Bacillus spp. for antimicrobial activities. The plant growth regulator indole-3-acetic acid was also identified in the bacterial extracts. In vitro assays showed that VOCs from strain HA and diffusible compounds from strain A8a modified root development and increased fresh weight of A. thaliana. These compounds differentially activated several hormonal signaling pathways involved in development and defense responses in A. thaliana, such as auxin, jasmonic acid (JA) and salicylic acid (SA); genetic analyses suggested that developmental stimulation of the root system architecture by strain A8a was mediated by the auxin signaling pathway. Furthermore, both strains were able to enhance plant growth and decreased the symptoms of Fusarium wilt in A. thaliana when soil-inoculated. Collectively, our results evidence the potential of these two rhizobacterial strains and their metabolites as biocontrol agents of avocado pathogens and as biofertilizers.

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Peptide Antibiotics Produced by Bacillus Species: First Line of Attack in the Biocontrol of Plant Diseases

Cited by 5 publications

References 85 publications

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications

Biocontrol of fungal phytopathogens by Bacillus pumilus

Biocontrol and plant growth promoting traits of two avocado rhizobacteria are orchestrated by the emission of diffusible and volatile compounds

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