Peptide-based high-density lipoprotein promotes adipose tissue browning and restrains development of atherosclerosis and type 2 diabetes

Xu, Rui; Li, Shizhong; Shi, Mingfei; Li, Ziyun; Wang, Yin; Li, Jing; Li, Qiang; Klausen, Lasse Hyldgaard; An, Li; Zhao, Haiyu; Chen, Menglin; Hu, Jingqing; Dong, Mingdong; Li, Ning

doi:10.1016/j.nantod.2020.101054

Cited by 11 publications

(16 citation statements)

References 78 publications

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“…Thirty minutes after drug exposure, the hearts and aortas were quickly isolated and imaged with excitation at 430 nm and emission at 530 nm, which were established and tested to reflect the fluorescent imaging of curcumin. Since the recruitment of pHDL itself to the regions of plaque in vivo has been confirmed previously by immunohistochemistry, 21 we additionally assessed the plaque-localizing effects of pHDL at the level of entire tissue and whole animals using FITC-pHDL.…”

Section: Methodsmentioning

confidence: 90%

“…The construction of drug-loaded pHDL using the microfluidic device was modified according to our established protocols for the synthesis of pHDL nanoparticles, as described in detail previously. 21 Briefly, TanIIA- or curcumin-loaded pHDL nanoparticles were prepared by injecting into the middle channel of the microfluidic chip a mixture of DMPC with either TanIIA (50–1500 μg/mL) or curcumin (100–1000 μg/mL) dissolved in ethanol (5 mg/mL) at a rate of 1 mL/min. Solutions of mimetic peptides in ddH 2 O (0.2 mg/mL) were injected into two external channels at a rate between 2.5 and 10 mL/min for the optimal strategy.…”

Section: Methodsmentioning

confidence: 99%

“…Meanwhile, the produced pHDL nanoparticles share similar physicochemical and biological properties with native HDL in the prevention of AS development. 21 …”

Section: Introductionmentioning

confidence: 99%

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Peptide-Based HDL as an Effective Delivery System for Lipophilic Drugs to Restrain Atherosclerosis Development

Gao¹,

Li²,

Li³

et al. 2022

IJN

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Purpose Peptide-based high-density lipoprotein (pHDL) structurally and functionally resembles the natural HDL as anti-atherosclerosis (AS) therapies. Since pHDL contains a large hydrophobic core, this study aims to evaluate the potentials of pHDL as a hydrophobic drug carrier and the efficiency of drug-loaded pHDL in the control of AS. Methods The pHDL encapsulation of hydrophobic components from natural plants, including curcumin (Cur) and tanshinone IIA (TanIIA), was achieved using one-step microfluidics. Then, morphological features and loading efficiencies of pHDL-Cur and pHDL-TanIIA were determined by TEM and high-performance liquid chromatography (HPLC), respectively. Taking the fluorescence advantage of Cur, localizations of loaded Cur in pHDL were investigated by fluorescence quenchers, and recruitments of Cur to AS plaques were assessed with ex vivo imaging. Based on anti-inflammatory properties of TanIIA, pHDL-TanIIA was accordingly developed to evaluate the anti-AS effects through examinations of plasma lipid parameters and pathological alterations of plaque-associated regions. Results Both lipophilic Cur and TanIIA can be efficiently loaded into pHDL carriers. The resultant pHDL-Cur and pHDL-TanIIA inherit the homogeneous nano-disk structure of pHDL. By using pHDL-Cur, the encapsulated hydrophobics are tracked in the core of pHDL, and incorporations of Cur with pHDL vehicles greatly improve the bioavailability and association of Cur with AS plaques. Moreover, when loaded with TanIIA, which has established its role in anti-AS as an anti-inflammatory candidate, synergistic effects in reducing AS lesions and improving pathological alterations of main organs related to AS were achieved. Conclusion The pHDL system could potentially be applied for both imaging and therapy in animal models of AS. Benefits of pHDL-based drug delivery will potentially extend the application scenarios of bioactive chemicals from natural plants which are underutilized due to features like low bioavailability and facilitate the clinical translation of synthetic HDL therapies in HDL-associated disorders, including but not limited to AS.

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Section: Methodsmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Peptide-Based HDL as an Effective Delivery System for Lipophilic Drugs to Restrain Atherosclerosis Development

Gao¹,

Li²,

Li³

et al. 2022

IJN

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“…Moreover, the Wong group reported a similar study, where the authors developed a human microvascular endothelial cell (HMVEC) and THP-1 cell co-culture system to demonstrate the remarkable efficacy of miR-146a-loaded microparticles on EC inflammation and monocyte adhesion on HMVECs (107). In another study, Li et al also created EC and THP-1 co-culture systems to show that the newly developed peptide-based high-density lipoprotein (pHDL) could inhibit THP-1 cell adhesion and achieve a similar inhibition effect human HDL (108). Thus, these studies demonstrated an in vitro EC/monocyte platform for testing potential treatments for suppressing atherosclerosis initiation.…”

Section: Phenotypic Modulationmentioning

confidence: 99%

Recent Progress in in vitro Models for Atherosclerosis Studies

Zhang

Millican

Lynd

et al. 2022

Front. Cardiovasc. Med.

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Atherosclerosis is the primary cause of hardening and narrowing arteries, leading to cardiovascular disease accounting for the high mortality in the United States. For developing effective treatments for atherosclerosis, considerable efforts have been devoted to developing in vitro models. Compared to animal models, in vitro models can provide great opportunities to obtain data more efficiently, economically. Therefore, this review discusses the recent progress in in vitro models for atherosclerosis studies, including traditional two-dimensional (2D) systems cultured on the tissue culture plate, 2D cell sheets, and recently emerged microfluidic chip models with 2D culture. In addition, advanced in vitro three-dimensional models such as spheroids, cell-laden hydrogel constructs, tissue-engineered blood vessels, and vessel-on-a-chip will also be covered. Moreover, the functions of these models are also summarized along with model discussion. Lastly, the future perspectives of this field are discussed.

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“…Material Surfaces and Interfaces at the Nanoscale: From Theory to Application Material surfaces and interfaces at the nano scales have become an engaging subject of interdisciplinary research due to numerous promising applications in the last 2 decades. Highly sophisticated techniques and novel families of materials have emerged with explosive growth and convincing functions in catalysis (Jiang et al, 2021), energy (Janek and Zeier, 2016), environmental sciences (Kartal et al, 2010), biomedicals (Zhang et al, 2020;Xu et al, 2021), etc.. Developing theories behind observed materials performances is also vital to the sustainable success of this interdisciplinary field and the successful implementation of new materials and processes into the next generation of advanced materials.…”

Section: Editorial On the Research Topicmentioning

confidence: 99%

Editorial: Material Surfaces and Interfaces at the Nanoscale: From Theory to Application

Xia

Zhang

et al. 2021

Front. Chem.

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Peptide-based high-density lipoprotein promotes adipose tissue browning and restrains development of atherosclerosis and type 2 diabetes

Cited by 11 publications

References 78 publications

Peptide-Based HDL as an Effective Delivery System for Lipophilic Drugs to Restrain Atherosclerosis Development

Peptide-Based HDL as an Effective Delivery System for Lipophilic Drugs to Restrain Atherosclerosis Development

Recent Progress in in vitro Models for Atherosclerosis Studies

Editorial: Material Surfaces and Interfaces at the Nanoscale: From Theory to Application

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