Peptide-bicelle interaction: Following variations in size and morphology by a combined NMR-SAXS approach

Dudás, Erika F.; Wacha, András; Bóta, Attila; Bodor, Andrea

doi:10.1016/j.bbamem.2019.183095

Cited by 6 publications

(3 citation statements)

References 67 publications

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“…The successfulness of the SLB formation using bicelles depends on the ratio of the long/short-chain PLs (q-ratio), total lipid concentration, lipid composition, type of substrate and NaCl concentration [ 73 , 75 , 76 ]. Using epifluorescence microscopy, it has been shown that bicelle-mediated SLBs with high Chol content form homogenous SLBs, unlike the SALB method where there are regions with no signal.…”

Section: Slbs With High Chol Concentrationsmentioning

confidence: 99%

Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains

et al. 2023

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Cholesterol (Chol) is an essential component of animal cell membranes and is most abundant in plasma membranes (PMs) where its concentration typically ranges from 10 to 30 mol%. However, in red blood cells and Schwann cells, PMs Chol content is as high as 50 mol%, and in the PMs of the eye lens fiber cells, it can reach up to 66 mol%. Being amphiphilic, Chol molecules are easily incorporated into the lipid bilayer where they affect the membrane lateral organization and transmembrane physical properties. In the aqueous phase, Chol cannot form free bilayers by itself. However, pure Chol bilayer domains (CBDs) can form in lipid bilayer membranes with the Chol content exceeding 50 mol%. The range of Chol concentrations surpassing 50 mol% is less frequent in biological membranes and is consequently less investigated. Nevertheless, it is significant for the normal functioning of the eye lens and understanding how Chol plaques form in atherosclerosis. The most commonly used membrane models are unilamellar and multilamellar vesicles (MLVs) and supported lipid bilayers (SLBs). CBDs have been observed directly using confocal microscopy, X-ray reflectometry and saturation recovery electron paramagnetic resonance (SR EPR). Indirect evidence of CBDs has also been reported by using atomic force microscopy (AFM) and fluorescence recovery after photobleaching (FRAP) experiments. The overall goal of this review is to demonstrate the advantages and limitations of the various membrane models and experimental techniques suitable for the detection and investigation of the lateral organization, function and physical properties of CBDs.

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Section: Slbs With High Chol Concentrationsmentioning

confidence: 99%

Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains

et al. 2023

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“…These structures spontaneously assemble by mixing phospholipids and a detergent at a given lipid:detergent molar ratio (q), which determines the particle size, and their properties vary depending on experimental conditions, such as lipid and detergent type and concentration, buffer ionic strength, and temperature [ 1 , 2 , 3 ]. Their high compositional tunability, along with stability against several freeze–thawing cycles, makes bicelles popular as models of cellular membranes for the study of both transmembrane protein structure and functioning and their intermolecular interactions in a native-like environment [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

et al. 2022

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Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.

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“…For definiteness, here we develop the statistical model for conductivity of the membrane induced by gramicidin A, although the consideration can be generalized for other types of AMPs inducing elastic deformations. As potential amplifiers of the gA activity, we consider single-pass transmembrane peptides, e.g., WALP or KALP [ 48 , 49 , 50 ], and short amphipathic peptides. Both type of peptides (denoted as I, inclusion) are supposed to carry a chemical group responsible for the selective binding to bacterial membranes.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Antimicrobial Peptide Activity via Tuning Deformation Fields by Membrane-Deforming Inclusions

Kondrashov

Akimov

2021

IJMS

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Antimicrobial peptides (AMPs) are considered prospective antibiotics. Some AMPs fight bacteria via cooperative formation of pores in their plasma membranes. Most AMPs at their working concentrations can induce lysis of eukaryotic cells as well. Gramicidin A (gA) is a peptide, the transmembrane dimers of which form cation-selective channels in membranes. It is highly toxic for mammalians as being majorly hydrophobic gA incorporates and induces leakage of both bacterial and eukaryotic cell membranes. Both pore-forming AMPs and gA deform the membrane. Here we suggest a possible way to reduce the working concentrations of AMPs at the expense of application of highly-selective amplifiers of AMP activity in target membranes. The amplifiers should alter the deformation fields in the membrane in a way favoring the membrane-permeabilizing states. We developed the statistical model that allows describing the effect of membrane-deforming inclusions on the equilibrium between AMP monomers and cooperative membrane-permeabilizing structures. On the example of gA monomer-dimer equilibrium, the model predicts that amphipathic peptides and short transmembrane peptides playing the role of the membrane-deforming inclusions, even in low concentration can substantially increase the lifetime and average number of gA channels.

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Peptide-bicelle interaction: Following variations in size and morphology by a combined NMR-SAXS approach

Cited by 6 publications

References 67 publications

Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains

Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

Regulation of Antimicrobial Peptide Activity via Tuning Deformation Fields by Membrane-Deforming Inclusions

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