Peptide enzyme‐linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine

Qi, Weier; Lin, Zhixian; Wu, Jinsen; Qian, Kun; Shao, Hongbo; Ye, Jianqiang; Qin, Aijian

doi:10.1186/s12917-021-02757-5

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…Respiratory and renal problems are observed in broiler and laying hens which generate economic losses in the poultry industry; although there are available vaccines, the immunization level should be evaluated, the pELISA showed that no cross-reactivity in immune sera against other viruses was detected, likewise, when compared with IFA the pELISA showed 98.15% sensitivity and 93.1% specificity. A positive correlation between the pELISA titers and neutralization titers suggests that this technique has the potential to replace neutralization assays when evaluating vaccines [ 67 ].…”

Section: Peptides In Diagnosismentioning

confidence: 99%

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

Montes-de-Oca-Jiménez²,

Vázquez-Chagoyán³

et al. 2022

Veterinary Sciences

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Peptides constitute an alternative and interesting option to develop treatments, vaccines, and diagnostic tools as they demonstrate their scope in several health aspects; as proof of this, commercial peptides for humans and animals are available on the market and used daily. This review aimed to know the role of peptides in the field of veterinary diagnosis, and include peptide-based enzyme-linked immunosorbent assay (pELISA), lateral flow devices, and peptide latex agglutination tests that have been developed to detect several pathogens including viruses and bacteria of health and production relevance in domestic animals. Studies in cattle, small ruminants, dogs, cats, poultry, horses, and even aquatic organisms were reviewed. Different studies showed good levels of sensitivity and specificity against their target, moreover, comparisons with commercial kits and official tests were performed which allowed appraising their performance. Chemical synthesis, recombinant DNA technology, and enzymatic synthesis were reviewed as well as their advantages and drawbacks. In addition, we discussed the intrinsic limitations such as the small size or affinity to polystyrene membrane and mention several strategies to overcome these problems. The use of peptides will increase in the coming years and their utility for diagnostic purposes in animals must be evaluated.

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Section: Peptides In Diagnosismentioning

confidence: 99%

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

Montes-de-Oca-Jiménez²,

Vázquez-Chagoyán³

et al. 2022

Veterinary Sciences

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“…Epitope is recognized by neutralizing antibodies that target S1 and N-gene to stimulate or neutralize antibodies and CTL responses [54]. Currently, one group of researchers has developed and tested a novel IBV vaccine based on the multiple epitopes from S1 and N protein coding genes [71,72]. This specific combination of multiple peptides generated a significant number of humoral and cell-mediated immune responses, which resulted in more than 80% protection after challenge with an infectious virus [73].…”

Section: Subunit and Peptide-based Vaccinesmentioning

confidence: 99%

Infectious Bronchitis Virus (Gammacoronavirus) in Poultry Farming: Vaccination, Immune Response and Measures for Mitigation

Bhuiyan

Amin

Rodrigues

et al. 2021

Veterinary Sciences

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Infectious bronchitis virus (IBV) poses significant financial and biosecurity challenges to the commercial poultry farming industry. IBV is the causative agent of multi-systemic infection in the respiratory, reproductive and renal systems, which is similar to the symptoms of various viral and bacterial diseases reported in chickens. The avian immune system manifests the ability to respond to subsequent exposure with an antigen by stimulating mucosal, humoral and cell-mediated immunity. However, the immune response against IBV presents a dilemma due to the similarities between the different serotypes that infect poultry. Currently, the live attenuated and killed vaccines are applied for the control of IBV infection; however, the continual emergence of IB variants with rapidly evolving genetic variants increases the risk of outbreaks in intensive poultry farms. This review aims to focus on IBV challenge–infection, route and delivery of vaccines and vaccine-induced immune responses to IBV. Various commercial vaccines currently have been developed against IBV protection for accurate evaluation depending on the local situation. This review also highlights and updates the limitations in controlling IBV infection in poultry with issues pertaining to antiviral therapy and good biosecurity practices, which may aid in establishing good biorisk management protocols for its control and which will, in turn, result in a reduction in economic losses attributed to IBV infection.

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Pathogenicity and molecular characterization of a GI-19 infectious bronchitis virus isolated from East China

Wu,

Xu,

Wei

et al. 2024

Front. Vet. Sci.

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Infectious bronchitis virus (IBV) is responsible for avian infectious bronchitis, a disease prevalent in countries with intensive poultry farming practices. Given the presence of multiple genotypic strains in China, identifying the regionally dominant genotypes is crucial for the implementation of effective prevention and control measures. This study focuses on the IBV strain CK/CH/WJ/215, isolated from a diseased commercial chicken flock in China in 2021. The CK/CH/WJ/215 isolate was genetically characterized through complete S1 sequence analysis. Phylogenetic comparisons were made with prevalent vaccine strains (H120, LDT3-A, and 4/91). Glycosylation patterns in the S1 protein were also analyzed. Pathogenicity was assessed in 7-day-old specific-pathogen-free chicks, monitoring morbidity, mortality, and tissue tropisms. Phylogenetic analysis clustered the CK/CH/WJ/215 isolate within the GI-19 lineage. Identity with the vaccination strains H120, LDT3-A, and 4/91 was low (75.7%, 78.6%, and 77.5% respectively). Novel glycosylation sites at positions 138 and 530 were identified compared to H120 and LDT-A. The isolate demonstrated nephropathogenic characteristics, causing 100% morbidity and 73.3% mortality in SPF chicks, with broader tropisms in tissues including trachea, lungs, kidneys, and bursa of Fabricius. Comprehensive genetic and pathological investigations revealed significant differences between the CK/CH/WJ/215 isolate and common vaccine strains, including novel glycosylation sites and a strong multiorgan infective capability. These findings are crucial for understanding the evolutionary dynamics of IBV and developing more effective prevention and control strategies.

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Peptide enzyme‐linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine

Cited by 3 publications

References 44 publications

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

The Use of Peptides in Veterinary Serodiagnosis of Infectious Diseases: A Review

Infectious Bronchitis Virus (Gammacoronavirus) in Poultry Farming: Vaccination, Immune Response and Measures for Mitigation

Pathogenicity and molecular characterization of a GI-19 infectious bronchitis virus isolated from East China

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