Peptide mapping of the ribulose bisphosphate carboxylase small subunit from the somatic hybrid of tomato and potato

Poulsen, Carsten Stig; Porath, Dan; Sacristán, M. D.; Melchers, Georg

doi:10.1007/bf02906178

Cited by 30 publications

(6 citation statements)

References 17 publications

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“…Carica papaya L. leaves were obtained from a plant grown under continuous light (1000 lux) at 20 ~ Two somatic hybrids of Solanum tuberosum L., dihaploid stock HH 258 and Lycopersicon esculentum Mill var. cerasiforme, mutant yellow green 6, RICK, were derived from the plants described by MELCHERS et al (14) and POUtSEN et al (22). The hybrids were 7c/13/$25 with 58 chromosomes and potato chloroplast DNA, and 2a/2a/36d/S3 with 49 (48) chromosomes and tomato chloroplast DNA (22).…”

Section: Plant Materialsmentioning

confidence: 99%

“…cerasiforme, mutant yellow green 6, RICK, were derived from the plants described by MELCHERS et al (14) and POUtSEN et al (22). The hybrids were 7c/13/$25 with 58 chromosomes and potato chloroplast DNA, and 2a/2a/36d/S3 with 49 (48) chromosomes and tomato chloroplast DNA (22). Parent and hybrid plants were grown in a green-house with a day temperature of 25 • 2 ~ and a night temperature of 20 • 2 ~ Leaves of Pisum sativum L. were obtained from plants grown in a growth chamber with a 16 hour day at 28 ~ (6700 lux) and an 8 hour night at 24 ~…”

Section: Plant Materialsmentioning

confidence: 99%

“…The recent production of somatic hybrids of potato and tomato (14,22) has provided interesting material for the study of chilling tolerance since one parent is chilling tolerant (potato) while the other (tomato) is chilling sensitive (29). Chlorophyll fluorescence photography has also been used to compare chilling tolerance of domestic tomatos with altitudinal forms of the wild tomato L. Hirsutum (R. PAULL and W. E. RtJsmoN, pers.…”

Section: Chilling Sensitivitymentioning

confidence: 99%

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Chlorophyll fluorescence photography to detect mutants, chilling injury and heat stress

Gibbons

Smillie

1980

Carlsberg Res. Commun.

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Chlorophyll fluorescence photography with high speed colour film has been used to examine: (i) kinetics of the rise and fall of variable fluorescence in barley leaves, (ii) detection of high fluorescing mendelian and maternally inherited mutants of barley, (iii) detection of protochlorophyllide-deflcient and protochlorophyllideaccumulating mutants of barley, (iv) heat stress by photographing the increase of initial fluorescence in heated leaves, and (v) decrease in variable fluorescence in leaves of peanut, tomato and tomato-potato somatic hybrids associated with the development of chilling injury. Filter combinations and a light source for photographing chlorophyll fluorescence are described. The relative intensity and kinetics of the fluorescence from the plants photographed was also measured, so that this could be correlated with fluorescence as detected by photography, and to facilitate the determination of optimal conditions for fluorescence photography.Abbreviations: Fo = initial fluorescence; Fv = variable fluorescence; Fs = steady state fluorescence.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Chilling Sensitivitymentioning

confidence: 99%

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Chlorophyll fluorescence photography to detect mutants, chilling injury and heat stress

Gibbons

Smillie

1980

Carlsberg Res. Commun.

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“…RuBisCO was purified from photoautotrophic Euglena (11), treated with SDS and the subunits separated on a Sephadex G-100 column (2.5 x 55 cm) (12). Fractions containing the SSU were pooled, lyophilized and then solubilized in glass-distilled water.…”

Section: Purification Of Rubisco and Its Subunitsmentioning

confidence: 99%

Amino acid sequence of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit from Euglena

Sailland¹,

Amiri

Freyssinet³

1986

Plant Mol Biol

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The amino acid sequence of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) small subunit (SSU) from Euglena has been established by alignment of the sequence of peptides obtained by cleavage with chymotrypsin, trypsin, Staphylococcus aureus protease or formic acid. The Euglena SSU has 138 amino acids and thus represents longest SSU sequence described so far. Homology is only 41% with cyanobacteria SSU and about 51% with higher plant SSU, whereas it is around 75% between higher plants. The largest homologous portion between all the known SSU sequences is localized in the second half and covers about 20 amino acids. The phylogenetic tree based on known SSU sequences has been established and the rate of amino acid substitution for SSU is estimated to be about 1.35×10(-9) per year and per site. Despite heterogeneity in amino acid sequence, we found that the overall secondary structure is fairly well conserved.

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“…Symmetric hybrids between tomato and potato, produced by protoplast fusion (3)(4)(5), showed an intermediate cold tolerance (6). Substitution of Solanum acaule genome into Solanum tuberosum could result in an appreciable increase of frost resistance (7,8).…”

mentioning

confidence: 99%

One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts.

Melchers

Mohri

Watanabe

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Mesophyll protoplasts of Lycopersicon esculentum were treated with iodoacetamide to inactivate mitochondria, and protoplasts of Solanum acauk and Solanum tuberosum were irradiated with v-or x-rays to inactivate nuclei. Mixtures of protoplasts thus modified were treated with Ca2+ and polyethylene glycol to obtain heterologous fusion products. Among the fusion products were some tomato plants that were indistinguishabl from the original cultivars with respect to morphology, physiology, and chromosome number (2N = 24) but exhibited various degrees of male sterility (MS): complete lack or malformation of anthers, shrunken pollen, and normal-looking stainable pollen that could not germinate. The MS thus induced in five cultivars ofdifferent growth types, including one of subspecies L. escukntum cerasforme, was inherited maternally over several generations and is, therefore, cytoplasmically determined MS (CMS). Analysis of mitochondrial DNA revealed that the mitochondrial genome of the CMS hybrids does not contain all elements ofthe mitochondrial DNA of either parent but includes sequences of a recombinational nature not present in either parent. The CMS hybrids, therefore, possess a true hybrid mitochondrial genome. The same procedure applied to fusion of tomato with Solaxum lycopersicoides and Nicoiana Atbacum cells did not produce CMS phenotypes. The advantages of this method &ver others for generating MS are as follows: (i) only one step is required; (i) the nuclear genotype of the cultivar is unaffected; (ija the prospect that cytoplasmic determination allows generation of 100% CMS progenies. The normal-appearing but nonfunctional pollen ofcertain CMS types might render them attractive to pollinating bumblebees that thus would facilitate production of hybrid seed.Male sterility (MS) can be artificially introduced into flowering plants by chemical treatment, by delivery of nuclear and/or organellar genes (1), or by transformation with a chimeric gene composed of an anther-specific promoter and a ribonuclease gene (2). In the production of hybrid seed, the need for MS plants is increasing to avoid manual emasculation offemale parents, as =40%o of the total labor is expended for this task, and to avert contamination by self-pollination. In conventional breeding, however, the use of genic MS requires many generations of backcrossing and selection from original sources. A method to produce MS in one step within a desired cultivar would, therefore, be extremely useful.Symmetric hybrids between tomato and potato, produced by protoplast fusion (3-5), showed an intermediate cold tolerance (6). Substitution of Solanum acaule genome into Solanum tuberosum could result in an appreciable increase of frost resistance (7,8). Subsequently, experiments were initiated to compare different fusion methods in this system. For this purpose tomato protoplasts were inactivated with iodoacetamide (IQA), which damages mitochondria and seems not to affect nuclear genomes, and the fusion partners of S. acaule or S. tuberosum were tr...

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Peptide mapping of the ribulose bisphosphate carboxylase small subunit from the somatic hybrid of tomato and potato

Cited by 30 publications

References 17 publications

Chlorophyll fluorescence photography to detect mutants, chilling injury and heat stress

Chlorophyll fluorescence photography to detect mutants, chilling injury and heat stress

Amino acid sequence of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit from Euglena

One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts.

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