Peptide Nucleic Acid–Modified Carbon Nanotube Field-Effect Transistor for Ultra-Sensitive Real-Time Detection of DNA Hybridization

Kerman, Kağan; Morita, Yasutaka; Takamura, Yuzuru; Tamiya, Eiichi; Maehashi, Kenzo; Matsumoto, Kazuhiko

doi:10.1385/nbt:1:1:065

Cited by 28 publications

(11 citation statements)

References 32 publications

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“…Another CNT‐FET biosensor for the electrical detection of DNA hybridization, real‐time and label‐free, based on the use of a SAM of peptide nucleic acid (PNA) probes has been developed . PNAs, a synthetic polymer in which the phosphates and deoxyriboses of the DNA backbone are replaced by a polypeptide, are frequently exploited in genetic sensors.…”

Section: Nanowire and Nanotube Dna Sensorsmentioning

confidence: 99%

Nanoprobes for enhanced electrochemical DNA sensors

Zaffino

Galán

Pardo

et al. 2015

WIREs Nanomed Nanobiotechnol

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Biosensors, small devices enabling selective bioanalysis because of properly assembled biological recognition molecules, represent the fortuitous results of years of interdisciplinary and complementary investigations in different fields of science. The ultimate role of a biosensor is to provide coupling between the recognition element and the analyte of interest, bringing a quantitative value of its concentrations into a complex sample matrix. They offer many advantages. Among them, portability, low cost with fast response times, and the possibility to operate in situ without the need for sample preparation are certainly the most important. Among biosensors, a large space is occupied by DNA biosensors. Screening genomic DNA is of fundamental importance for the development of new tools available to physicians during the clinical process. Sequencing of individual human genomes, accomplished principally by microarrays with optical detection, is complex and expensive for current clinical protocols. Efforts in research are focused on simplifying and reducing the cost of DNA biosensors. For this purpose, other transduction techniques are under study to make more portable and affordable DNA biosensors. Compared with traditional optical detection tools, electrochemical methods allow the same sensitivity and specificity but are less expensive and less labor intensive. Scalability of electrochemical devices makes it possible to use the advantages introduced by nanosized components. The involvement of nanomaterials and nanostructures with custom-tailored shapes and properties is expected to rapidly boost the field of electrochemical DNA biosensors and, in general, that of next-generation sequencing technologies.

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Section: Nanowire and Nanotube Dna Sensorsmentioning

confidence: 99%

Nanoprobes for enhanced electrochemical DNA sensors

Zaffino

Galán

Pardo

et al. 2015

WIREs Nanomed Nanobiotechnol

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“…Following a similar approach, Matsumoto and coworkers reported on the engineering of a CNT-based field-effect transistor (FET) in which the reverse side of the CNTs-based electrode has been coupled to ssPNA-bearing SAMs as detecting probes conjugated to the tumor necrosis factor-α-gene (TNF-α) 55 . Upon exposure to wild-type DNA samples through microfluidic channels, time-dependent conductance measurements enabled rapid and simple discrimination against single-nucleotide polymorphism or non-cDNA strands.…”

Section: Sspna-cnts Hybridsmentioning

confidence: 99%

Peptide nucleic acids in materials science

Bonifazi

Carloni

Corvaglia

et al. 2012

Artificial DNA: PNA & XNA

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This review highlights the recent methods to prepare PNA-based materials through a combination of self-assembly and self-organization processes. The use of these methods allows easy and versatile preparation of structured hybrid materials showing specific recognition properties and unique physicochemical properties at the nano- and micro-scale levels displaying potential applications in several directions, ranging from sensors and microarrays to nanostructured devices for biochips.

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“…Recently, detection of virus‐specific DNA sequences has been successfully demonstrated with ssDNA‐immobilized nanomaterial‐based sensor platforms using mwCNT [56], and SiNW [57]. Furthermore, nanomaterial‐based DNA sensors were also successfully applied for the detection of DNA damage [58] and single nucleotide polymorphisms (SNP) [59]. Advances in the development of various types of DNA biosensors could allow them to be used for many different applications including disease diagnosis and the detection of genetic disorders.…”

Section: Types Of Biomolecules For Functionalization Of Sensor Platmentioning

confidence: 99%

Integration of biomolecules and nanomaterials: Towards highly selective and sensitive biosensors

Seok

Park

2011

Biotechnology Journal

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Significant efforts have been made toward the development of high-performance biosensors for various applications. Advances in nanotechnology have resulted in the development of highly sensitive electrochemical sensing devices. It is believed that highly sensitive and selective biosensors can be realized through the integration of biomolecules and nanomaterial-based sensor platforms. Numerous articles have described combining biomolecules as recognition elements with nanotechnology for the development of biosensors with enhanced selectivity and sensitivity. Recent advances in the development of biosensors through the integration of biomolecules with nanotechnology are reviewed in this article.

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Peptide Nucleic Acid–Modified Carbon Nanotube Field-Effect Transistor for Ultra-Sensitive Real-Time Detection of DNA Hybridization

Cited by 28 publications

References 32 publications

Nanoprobes for enhanced electrochemical DNA sensors

Nanoprobes for enhanced electrochemical DNA sensors

Peptide nucleic acids in materials science

Integration of biomolecules and nanomaterials: Towards highly selective and sensitive biosensors

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