Peptide purity and counter ion determination of bradykinin by high-performance liquid chromatography and capillary electrophoresis

Ridge, Shane; Hettiarachchi, Kanthi

doi:10.1016/s0021-9673(98)00313-6

Cited by 31 publications

(16 citation statements)

References 21 publications

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“…The Ar 1 laser with excitation at 488 nm and emission at 535 nm is the most popular and commercially available. Few peptides contain a native fluorophore, thus for CE-LIF detection most peptides need to be derivatized with a fluorescent probe [23][24][25]. Siri et al [23] reported an automated large-volume sample stacking (LVSS) procedure to detect FITC-labeled BK and other peptides in the picomolar range.…”

Section: Introductionmentioning

confidence: 99%

Assay of bradykinin‐related peptides in human body fluids using capillary electrophoresis with laser‐induced fluorescence detection

Chen

Lin

et al. 2008

Electrophoresis

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A CE with LIF detection was developed for separation and determination of bradykinin (BK)-related peptides, such as BK, kallidin (Kal), and neurokinin A (NKA). BK-related peptides were derivatized with FITC prior to CE-LIF analysis. Sodium borate 10 mmol/L at pH 9.5 was selected as derivatization media in order to get the high efficiency. Three peptides were baseline-separated within 10 min by using 110 mmol/L sodium borate-sodium hydroxide solution at pH 10.0 as the running buffer. Concentration detection limits (S/N = 3) for BK, Kal, and NKA were 0.08, 0.5, and 0.2 nmol/L, respectively. Meanwhile we have also developed a simple, quick, and sensitive large-volume sample stacking (LVSS) technique for CE-LIF detection of BK, Kal, and NKA. By using this stacking technique, the detection limits (S/N = 3) for BK, Kal, and NKA were 0.02, 0.05, and 0.04 nmol/L, respectively. This method has been applied to the assay of human saliva and cerebrospinal fluid with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Assay of bradykinin‐related peptides in human body fluids using capillary electrophoresis with laser‐induced fluorescence detection

Chen

Lin

et al. 2008

Electrophoresis

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“…Many different probes have been reported as components of the BGE for indirect detection. One of them is phthalate that was first suggested for UV indirect detection in 1991 [3,4] and since that time it has been reported in many papers for the analysis of inorganic [5][6][7] and small organic anions [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25], and scarcely also for other classes of substances such as glycolysis metabolites [26], nucleotides [27], or peptides [28].…”

Section: Introductionmentioning

confidence: 99%

“…This paper is aimed at providing an insight into the basic electromigration properties of BGEs based on phthalate that might have remained mostly unrecognized so far. This should provide a guide for an optimum architecture of BGEs containing phthalate that are frequently used in analytical practice and were already also suggested as robust BGEs for routine CZE applications in pharmaceutical analysis [28].…”

Section: Introductionmentioning

confidence: 99%

Success and failure with phthalate buffers in capillary zone electrophoresis

2001

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Phthalate buffers are currently used in capillary electrophoresis as robust electrolyte systems for indirect detection. This contribution demonstrates that these buffers show regularly not only successful regions of mobilities of analytes (sample window) but also regions of failure where the migration of analytes is strongly deteriorated due to the presence of a system zone. System zones in phthalate buffers may be easily detected by UV detection and manifest themselves as peaks or dips. Peak shape diagrams are advantageously used for the prediction of the migration behavior of system zones in phthalate background electrolyte (BGE) systems at various pH. It is shown that the mobility of the system zone varies strongly with pH, is practically zero at pH values below 4 and above 7, and shows a maximum at pH 5. Thus, the system peak may coincide either with the peaks of various analytes or with the electroosmotic flow (EOF) peak. Experiments are given showing the effects of such coincidences as, e.g., zigzag detection patterns, double EOF peaks, and/or unusually broad peaks/dips. The message of this contribution is to show how to understand the electrophoretic properties of phthalate BGEs that, regardless of possible failure regions, may be successfully used in the analytical practice of capillary zone electrophoresis (CZE).

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“…CE in buffers at pH 9.35 and pH 2.70 has been used for the determination of the dipeptide sweetener aspartame (a-L-Asp-L-Phe-O-Me) and of its potential degradation products, such as Phe, Phe-O-Me [62]. CE was instrumental in assessing the purity of the synthetic nonapeptide hormone bradykinin [63], for analysis of cyclic peptide growth promoters [64], of thymosins [65], of lipopeptides [66] and ginseng polypeptides [67]. Three opioid peptides, b-endorphin, Met-enkephalin, Leu-enkephalin and four other neuropeptides, substance P, somatostatin, vasopressin and oxytocin, all of them related to pain mechanism in the vertebrate central nervous system, were determined by CE in rat brain tissue homogenates and plasma, as well as in human plasma, with detection limits in the subpicomole level (68).…”

Section: Permanent and Dynamic Coatings For Proper Peptide/protein Sementioning

confidence: 99%

Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest

Righetti

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is an automated approach to electrokinetic separations that has had a deep impact in all fields of life sciences, including biomedical and biotechnological research and clinical and forensic practice. The present review highlights aspects of peptides and proteins separations, with particular emphasis on macromolecular analytes of biomedical interest. Among the various CE techniques available, a novel methodology is here illustrated consisting in separations in acidic, isoelectric buffers, which have the advantage of protonating the silica wall, thus minimizing interactions of proteinaceous material with the siliceous surface, while allowing delivery of high voltage gradients, due to their low conductivities. The review ends with applications of CE to the analysis of folding/unfolding/refolding/misfolding of proteins, a field which has deep implications in the biomedical arena, since it is connected to a host of disorders, such as prion protein diseases.

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Peptide purity and counter ion determination of bradykinin by high-performance liquid chromatography and capillary electrophoresis

Cited by 31 publications

References 21 publications

Assay of bradykinin‐related peptides in human body fluids using capillary electrophoresis with laser‐induced fluorescence detection

Assay of bradykinin‐related peptides in human body fluids using capillary electrophoresis with laser‐induced fluorescence detection

Success and failure with phthalate buffers in capillary zone electrophoresis

Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest

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