2021
DOI: 10.1002/cbic.202000845
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Peptide Vectors Carry Pyrene to Cell Organelles Allowing Real‐Time Quantification of Free Radicals in Mitochondria by Time‐Resolved Fluorescence Microscopy

Abstract: Real‐time quantification of reactive nitrogen and oxygen species (ROS) in cells is of paramount importance as they are essential for cellular functions. Their excessive formation contributes to the dysfunction of cells and organisms, ultimately leading to cell death. As ROS are mostly produced in the mitochondria, we have synthesized a fluorescent probe able to reach this organelle to detect and quantify, in real time, the variation of ROS by time‐resolved microfluorimetry. The new probes are based on the long… Show more

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Cited by 11 publications
(10 citation statements)
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“…Thus, these particular pyrene analogues, combining fluorescent emission with photoinduced bioactivity, can be considered promising lead compounds for the development of photo-induced peptide-based theranostic agents [34], particularly in PDT therapy under two-photon absorption (TPA) [39][40][41][42][43] conditions. The obtained results, as well as very recently published advances in pyrene-carrying peptide vectors for detection of free radicals in cell organelles [55], strongly support further biological studies focused on the exact intracellular location of the pyrene peptidoids 6 and 7, as well as their new analogues, and study of the mechanism of action using analysis of photo-induced intracellular ROS species.…”
Section: Discussionsupporting
confidence: 78%
“…Thus, these particular pyrene analogues, combining fluorescent emission with photoinduced bioactivity, can be considered promising lead compounds for the development of photo-induced peptide-based theranostic agents [34], particularly in PDT therapy under two-photon absorption (TPA) [39][40][41][42][43] conditions. The obtained results, as well as very recently published advances in pyrene-carrying peptide vectors for detection of free radicals in cell organelles [55], strongly support further biological studies focused on the exact intracellular location of the pyrene peptidoids 6 and 7, as well as their new analogues, and study of the mechanism of action using analysis of photo-induced intracellular ROS species.…”
Section: Discussionsupporting
confidence: 78%
“…In this paper, we employed a pyren-2-yl moiety as the aryl group because the uorescence emissions from 2-substituted pyrenes show intrinsic vibrational structures and long lifetimes, [51][52][53][54][55][56][57][58][59][60][61] which could provide adequate time to allow the LE and CT states to equilibrate. We studied the photoinduced CT process and the thermodynamic equilibrium between the CT Fig.…”
Section: Introductionmentioning
confidence: 99%
“…In this paper, we employed a pyren-2-yl moiety as the aryl group because the fluorescence emissions from 2-substituted pyrenes show intrinsic vibrational structures and long lifetimes, 51–61 which could provide adequate time to allow the LE and CT states to equilibrate. We studied the photoinduced CT process and the thermodynamic equilibrium between the CT state and the thermally activated LE states by combining temperature-dependent luminescence spectroscopy, fs- and ns-transient absorption spectroscopy, and DFT and LR-TDDFT calculations.…”
Section: Introductionmentioning
confidence: 99%
“…Two pyrene-based probes, C33 and C34 (see Figure 16 f; 1-PBA and 2-PBA are the corresponding representations in their original reports) were reported as effective candidates for the mitochondria imaging studies [ 101 ]. This work elaborated a carrier role of the peptide vectors, which carried the C33 and C34 molecules into cell organelles.…”
Section: Aie-tuned Bioimaging From Pyrene-based Sensory Probesmentioning
confidence: 99%