Peptides as Therapeutic Agents for Dengue Virus

Chew, Miaw Fang; Poh, Keat Seong; Poh, Chit Laa

doi:10.7150/ijms.21875

Cited by 64 publications

(54 citation statements)

References 192 publications

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“…Its genome encodes three structural proteins: capsid (C), pre-membrane (prM) and envelope (E), and seven non-structural proteins (NSs). The E protein is glycosylated and has an important role in viral adsorption and entry, with 500 amino acids; a C-terminal that consist of a highly conserved stem region, formed by two helices (H1 and H2) and partially inserted in the lipid envelope; and finally, the N-terminal ectodomain formed by three domains (EDI, EDII, and EDII) [132]. DN59, a peptide mimetic to the E protein stem region, was tested against DENV2 and WNV, showing inhibition of both viruses at a concentration lower than 25 µM [133], and in a more recent assay, when tested against all four dengue serotypes, DN59 showed inhibition of infectivity by the release of the viral genome through holes formed in the envelope.…”

Section: Dengue Virus As a Scaffold For Peptide Designmentioning

confidence: 99%

Antiviral peptides as promising therapeutic drugs

Boas

Campos

Berlanda

et al. 2019

Cell. Mol. Life Sci.

254

175

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While scientific advances have led to large-scale production and widespread distribution of vaccines and antiviral drugs, viruses still remain a major cause of human diseases today. The ever-increasing reports of viral resistance and the emergence and re-emergence of viral epidemics pressure the health and scientific community to constantly find novel molecules with antiviral potential. This search involves numerous different approaches, and the use of antimicrobial peptides has presented itself as an interesting alternative. Even though the number of antimicrobial peptides with antiviral activity is still low, they already show immense potential to become pharmaceutically available antiviral drugs. Such peptides can originate from natural sources, such as those isolated from mammals and from animal venoms, or from artificial sources, when bioinformatics tools are used. This review aims to shed some light on antimicrobial peptides with antiviral activities against human viruses and update the data about the already well-known peptides that are still undergoing studies, emphasizing the most promising ones that may become medicines for clinical use.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Dengue Virus As a Scaffold For Peptide Designmentioning

confidence: 99%

Antiviral peptides as promising therapeutic drugs

Boas

Campos

Berlanda

et al. 2019

Cell. Mol. Life Sci.

254

175

View full text Add to dashboard Cite

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“…In the case of HSA proteins and DENV-1 E-proteins, the results indicated a least SPR response due to the non-specific antibody binding. The least response from DENV-1 E-proteins likely reflects the successful interactions of similar genome, which shares 65% of single-stranded RNA genomes encoded by other DENV serotypes 94,95 . As for HSA proteins, high SPR response can be accounted as an excessive proteins in the blood with a molecular weight of 66.4 kDa when compared to 50 kDa DENV-1 E-proteins 96,97 .…”

Section: Sensitivity and Binding Affinity Of Dsu/nh 2 Rgo-pamam/igm Smentioning

confidence: 99%

Sensitive Detection of Dengue Virus Type 2 E-Proteins Signals Using Self-Assembled Monolayers/Reduced Graphene Oxide-PAMAM Dendrimer Thin Film-SPR Optical Sensor

Omar

Fen

Abdullah

et al. 2020

Sci Rep

123

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In this work, sensitive detection of dengue virus type 2 E-proteins (DENV-2 E-proteins) was performed in the range of 0.08 pM to 0.5 pM. The successful DENV detection at very low concentration is a matter of concern for targeting the early detection after the onset of dengue symptoms. Here, we developed a SPR sensor based on self-assembled monolayer/reduced graphene oxide-polyamidoamine dendrimer (SAM/NH 2 rGO/PAMAM) thin film to detect DENV-2 E-proteins. Surface characterizations involving X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) confirms the incorporation of NH 2 rGO-PAMAM nanoparticles in the prepared sensor films. The specificity, sensitivity, binding affinity, and selectivity of the SPR sensor were then evaluated. Results indicated that the variation of the sensing layer due to different spin speed, time incubation, and concentration provided a better interaction between the analyte and sensing layer. The linear dependence of the SPR sensor showed good linearity (R 2 = 0.92) with the lowest detection of 0.08 pM DENV-2 E-proteins. By using the Langmuir model, the equilibrium association constant was obtained at very high value of 6.6844 TM −1 (R 2 = 0.99). High selectivity of the SPR sensor towards DENV-2 E-proteins was achieved in the presence of other competitors. Dengue virus (DENV) is the most common arthropod-borne viral disease that poses a serious global problem. According to World Health Organization (WHO), the dengue virus is the leading cause of death of 22 000, annually. As of today, the need for hospitalization and medical treatment are constantly dense due to the fact that 390 million people in the world are still infected with DENV 1. Its four distinct serotypes (DENV-1. DENV-2, DENV-3, and DENV-4) are capable of causing a range of clinical symptoms ranging from mild fevers to the severe dengue haemorrhagic fever (DHF) and be potentially life-threatening 2-10. Despite its large burden to human health, no effective vaccine and antiviral therapy are available for the virus 11,12. Early treatment for DENV is only by maintaining the body fluid of the patient, as it is critical in fighting the severe symptoms of DENV 13,14. Hence, an early, rapid, and accurate diagnosis at the onset of infection is the demand of the day in the most epidemic settings. Present discoveries in dengue diagnostics that can help in the early diagnosis are targeting the host-virus itself. DENV consists of a single-stranded positive-sense RNA virus that encodes 10 different types of proteins. Seven of

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“…This SARS-CoV-2 viral attack has become a worldwide emergency in different regions of the World, especially in China (Mcclain, 1995). As an immediate response, numerous efforts from all over the world have been made to develop a peptide based vaccine against SARS-CoV-2, and the peptide inhibitors are of great interest to develop vaccines (Chew et al, 2017;Usman Mirza et al, 2017). The peptide targets are more preferable than traditional ligand-based drugs and vaccines due to different aspects including less toxic, fewer side-effects and their ultrafast action.…”

Section: Discussionmentioning

confidence: 99%

Determine the Potential Epitope Based Peptide Vaccine Against Novel SARS-CoV-2 Targeting Structural Proteins Using Immunoinformatics Approaches

Waqas

Ali

Sufyan

et al. 2020

Front. Mol. Biosci.

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Coronaviruses (CoVs) belong to the Coronaviridae-family. The genus Betacoronaviruses, are enveloped positive strand RNA viruses with club-like spikes at the surface with a unique replication process and a large RNA genome (∼25 kb). CoVs are known as one of the major pathogenic viruses causing a variety of diseases in birds and mammals including humans (lethal respiratory dysfunctions). Recently, a new strain of coronavirus has been identified and named as SARS-CoV-2. A large number of COVID-19 (disease caused by SARS-CoV-2) cases are being diagnosed all over the World especially in China (Wuhan). COVID-19 showed high mortality rate exponentially, however, not even a single effective cure is being introduced yet against COVID-19. In the current study, immunoinformatics approaches were employed to predict the antigenic epitopes against COVID-19 for the development of a coronavirus peptide vaccine. Cytotoxic T-lymphocyte (CTL) and B-cell epitopes were predicted for SARS-CoV-2 coronavirus structural proteins (Spikes, Membrane, Envelope, and Nucleocapsid). The docking complexes of the top 10 epitopes having antigenic sites were analyzed led by binding affinity and binding interactional analyses of top ranked predicted peptides with the MHC-I HLA molecule. The predicted peptides may have potential to be used as peptide vaccine against COVID-19.

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Peptides as Therapeutic Agents for Dengue Virus

Cited by 64 publications

References 192 publications

Antiviral peptides as promising therapeutic drugs

Antiviral peptides as promising therapeutic drugs

Sensitive Detection of Dengue Virus Type 2 E-Proteins Signals Using Self-Assembled Monolayers/Reduced Graphene Oxide-PAMAM Dendrimer Thin Film-SPR Optical Sensor

Determine the Potential Epitope Based Peptide Vaccine Against Novel SARS-CoV-2 Targeting Structural Proteins Using Immunoinformatics Approaches

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