Peptidic Hormones in Annelids

M, Durchon

doi:10.1007/978-3-642-69922-1_2

Cited by 4 publications

(7 citation statements)

References 22 publications

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“…This assumption was mainly based on the results of partial characterisation (Durchon, 1984), as well as histological observations, by which the brain regions producing nereidin were shown to contain highly active secretory cells (Müller, 1973; Hofmann, 1976). To assess whether the recovered nereidin activity was of proteinaceous nature, we treated the obtained eluate with proteinase K, and tested if this affected the ability of the eluate to repress vtg levels in cell culture.…”

Section: Resultsmentioning

confidence: 99%

“…The identification of MF in Platynereis heads challenges several hypotheses: most directly, it defeats the hypothesis that nereidin is a peptide, a notion that has been propagated in the literature for more than 30 years (Cardon et al, 1981; Durchon, 1984). Furthermore, MF and related sesquiterpenoid hormones have so far only been found in arthropods (Bellés et al, 2005; Jindra et al, 2015), and their evolution has been hypothesised to be a secondary consequence of the loss of the sterol synthesis pathway in this clade (Zandee, 1964; Bellés et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…These finally resulted in the identification of a hexapeptide (PPGPPG). However, this peptide did not display biological activity (Durchon, 1984). …”

Section: Introductionmentioning

confidence: 99%

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Discovery of methylfarnesoate as the annelid brain hormone reveals an ancient role of sesquiterpenoids in reproduction

Schenk

Krauditsch

Frühauf

et al. 2016

eLife

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Animals require molecular signals to determine when to divert resources from somatic functions to reproduction. This decision is vital in animals that reproduce in an all-or-nothing mode, such as bristle worms: females committed to reproduction spend roughly half their body mass for yolk and egg production; following mass spawning, the parents die. An enigmatic brain hormone activity suppresses reproduction. We now identify this hormone as the sesquiterpenoid methylfarnesoate. Methylfarnesoate suppresses transcript levels of the yolk precursor Vitellogenin both in cell culture and in vivo, directly inhibiting a central energy–costly step of reproductive maturation. We reveal that contrary to common assumptions, sesquiterpenoids are ancient animal hormones present in marine and terrestrial lophotrochozoans. In turn, insecticides targeting this pathway suppress vitellogenesis in cultured worm cells. These findings challenge current views of animal hormone evolution, and indicate that non-target species and marine ecosystems are susceptible to commonly used insect larvicides.DOI: http://dx.doi.org/10.7554/eLife.17126.001

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Discovery of methylfarnesoate as the annelid brain hormone reveals an ancient role of sesquiterpenoids in reproduction

Schenk

Krauditsch

Frühauf

et al. 2016

eLife

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“…The coelomic contents, obtained by puncture of animals whose genital state had previously been determined by the method of Durchon [14], were centrifuged for 3 niin at 1000 x g . The sediment of cellular elements (oocytes-coelomocytes) was mixed with 2 ml of 0.05 M sodium phosphate pH 7.4 buffer containing 0.35 M NaCl and the cell suspension was layered above 5ml of a 5 % (w/v) Ficoll solution in the same buffer.…”

Section: Preparation Of Oocytesmentioning

confidence: 99%

Purification and characterization of oocyte vitellin from Perinereis cultrifera (polychaete annelid)

Baert

Sáutière

Porchet

1984

European Journal of Biochemistry

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Vitellin was identified and purified from submature oocytes of Perinereis cultrfera by concanavalin-ASepharose and Ultrogel AcA 34 column chromatography. The yolk protein was defined as a glycolipoprotein with a relative molecular mass of' 380000. Upon dissociation by sodium dodecyl sulphate, vitellin was shown to release five polypeptide subunits which ranged in relative molecular mass from 98000 -16000. The purified vitellin was further characterized by amino acid analysis and its lipid and carbohydrate contents. The molecule contained about 5 % carbohydrate and 16'x lipid. Antiserum prepared against vitellin was shown to react with a component from the coelomic fluid of maturing females. Thus, it was suggested that in nereid annelids, considered phylogenetically and morphologically primitive, oocyte differentiation might depend upon the incorporation of a vitellogenin as in insects and many oviparous vertebrates.The sexuality of Nereids (Polychaeta worms) has been extensively studied. Many authors have demonstrated the existence of a brain neurohormone which inhibits oogenesis and somatic transformations of the epitoky [l -31. Studying the endocrinal determination of oogenesis, Durchon and Porchet [4] found a correlation between the brain hormonal production and the oocyte metabolism. During the juvenile phase characterized by an important production of brain hormone, yolk granules appear in the oocytic ooplasm (vitellogenesis) whereas at the time of genital submaturity, in absence of brain hormone, the oocytes produce cortical alveoli.While hormonal determination of oogenesis and cytology of the oocytes have been the object of several biological investigations [4 -81, relatively few biochemical studies have been carried out on the oocyte metabolism among the Nereidae. Concerning vitellogenesis, yolk synthesis has been shown to occur within the oocyte from small molecular weight precursors such as amino acids and monosaccharides (process termed autosynthesis) in Nel-eis divcwicolor, Perinereis cultr(fet,9, 101. However, Fischer [Ill, using Nereis virens, demonstrated the presence of a vitellin-like antigen in the coelomic fluid of mature females suggesting the heterosynthetic origin of the yolk. In Perinereis cultrifkra, immunological studies by Dhainaut et al. [I21 revealed identical antigens in the oocytes and coelomic fluid of females, but no significant evidence of an involvement of this material in yolk synthesis was obtained.Among annelids, reports on yolk protein remain limited to N . virens vitellin [13]. This protein, isolated by a method including DEAE-cellulose and phosphocellulose chromatography, has been characterized as a lipoglycoprotein of apparent Mr of 420000 and from its amino acid composition, appears to resemble insect vitellins. Apart from these gross features, Nereis vitellin remains poorly characterized. With interest in the hormonal control of vitellin synthesis as well as in its evolutionary relationship among annelids and other invertebrates, further studies of vitellins are requi...

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“…The molecular identity of the brain hormone has remained elusive for many years. While initially believed to be a neuropeptide (Müller 1973;Hofmann 1976;Cardon et al 1981;Durchon 1984), it was recently found that nereidin activity in P. dumerilii involves methylfarnesoate (MF), a sesquiterpenoid hormone related to arthropod juvenile hormones (Schenk et al 2016).…”

Section: Introductionmentioning

confidence: 99%

On the hormonal control of posterior regeneration in the annelid Platynereis dumerilii

Álvarez‐Campos

Planques

Bideau

et al. 2022

Preprint

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Regeneration is the process by which many animals are able to restore lost or injured body parts. After amputation of the posterior part of its body, the annelid Platynereis dumerilii is able to regenerate the pygidium, the posteriormost part of its body that bears the anus, and a subterminal growth zone containing stem cells that allows the subsequent addition of new segments. The ability to regenerate their posterior part (posterior regeneration) is promoted in juvenile worms by a hormone produced by the brain and is lost when this hormonal activity becomes low at the time the worms undergo their sexual maturation. By characterizing posterior regeneration at the morphological and molecular levels in worms that have been decapitated, we show that the presence of the head is essential for multiple aspects of posterior regeneration, as well as for the subsequent production of new segments. We also show that methylfarnesoate, the molecule proposed to be the brain hormone, can partially rescue the posterior regeneration defects observed in decapitated worms. Our results are therefore consistent with a key role of brain hormonal activity in the control of regeneration and growth in P. dumerilii, and support the hypothesis of the involvement of methylfarnesoate in this control.

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Peptidic Hormones in Annelids

Cited by 4 publications

References 22 publications

Discovery of methylfarnesoate as the annelid brain hormone reveals an ancient role of sesquiterpenoids in reproduction

Discovery of methylfarnesoate as the annelid brain hormone reveals an ancient role of sesquiterpenoids in reproduction

Purification and characterization of oocyte vitellin from Perinereis cultrifera (polychaete annelid)

On the hormonal control of posterior regeneration in the annelid Platynereis dumerilii

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