Peptidomics

Hellinger, Roland; Sigurdsson, Arnar; Wenxin, Wu; Romanova, Elena V.; Li, Lingjun; Sweedler, Jonathan V.; Süssmuth, Roderich D.; Gruber, Christian W.

doi:10.1038/s43586-023-00205-2

Cited by 41 publications

(19 citation statements)

References 274 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tuning the nhPHIP system towards amino acids with pyridine as cosubstrate 28 involved heating the sample to 50 °C under strong basic conditions (pH 11) – a potentially undesirable combination due to possible hydrolysis and oxidation of peptides. 29 A screening of ten alternative cosubstrates with different steric and electronic properties 30 (SI_nhPHIP Fig. S1†) on a mixture of amino acids (Ala, Leu, Phe) under basic conditions revealed 3-fluoro-4-methylpyridine (3F4MePy) as optimal, since it forms the desired amino acid catalyst complex spontaneously at room temperature.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Parahydrogen hyperpolarized NMR detection of underivatized short oligopeptides

Reimets,

Ausmees,

Vija

et al. 2023

Analyst

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…This emerging field of research currently relies on mass spectroscopy coupled with HPLC or CE, where extensive sample preparation is needed to enrich short peptides over matrix compounds. 29 nhPHIP could be a relatively easy to implement alternative that allows to conduct peptidomics by NMR.…”

Section: Resultsmentioning

confidence: 99%

Parahydrogen hyperpolarized NMR detection of underivatized short oligopeptides

Reimets,

Ausmees,

Vija

et al. 2023

Analyst

View full text Add to dashboard Cite

show abstract

“…This could be accomplished by using genomic and transcriptomic techniques to identify biosynthetic gene clusters responsible for cyclic peptide production, as well as employing high-throughput assays to screen for new bioactive compounds [70,71]. Moreover, petidomics is an emerging discipline that integrates genomics, proteomics, analytical chemistry techniques such as liquid chromatography and mass spectrometry, and computational biology to qualitatively and quantitatively analyze biologically significant cyclic peptides [72].…”

Section: Future Perspectivesmentioning

confidence: 99%

Cyclic Peptides with Antifungal Properties Derived from Bacteria, Fungi, Natural Products, and Synthetic Sources

Helmy¹,

Parang²

2023

Preprint

View full text Add to dashboard Cite

Fungal infections pose a significant threat to human health, particularly in immunocompromised individuals. Due to several factors, such as the inappropriate use of antimicrobial drugs, the emergence of resistance in microorganisms, and increasing demand for less cytotoxic antifungal agents for immunocompromised patients, antifungal research has gained significant attention in recent years. Antifungal peptides, and among them, cyclic peptides, are a class of antifungal agents that have been under development since the 1960s. Using cyclic peptides as a promising approach for combating antifungal resistance in pathogenic fungi has gained significant attention in recent years. The identification of antifungal cyclic peptides from various sources has been possible due to the widespread interest in peptide research in recent decades. It is increasingly important to evaluate the narrow to broad-spectrum antifungal activity and the mode of action of synthetic and natural cyclic peptides for both synthesized and extracted peptides. This short review aims to highlight some of the antifungal cyclic peptides isolated from bacteria, fungi, and plants, taking into consideration the progress made in peptide research. Additionally, it provides a summary of synthetic cyclic peptides with antifungal activity and discusses the potential future of utilizing combinations of antifungal peptides from different sources. The review underscores the need for further exploration of the novel therapeutic applications of these abundant and diverse cyclic peptides.

show abstract

“…Mass spectrometry (MS)-based neuropeptidomics provides a powerful tool for profiling the neuropeptides at a global scale with high sensitivity and unparalleled molecular specificity. − Compared to traditional study methods such as radioimmunoassay, immunohistochemistry, and Edman degradation, the MS-based strategy can simultaneously detect a large number of neuropeptides in a high-throughput manner, also with successful identification of the neuropeptides carrying PTM. This method has been effectively applied across a broad spectrum of species within the animal kingdom, demonstrating its versatility and effectiveness. ,− Confined to the crustacean, our group, along with others, has leveraged mass spectrometry for the discovery and profiling of various neuropeptides. − However, the potential of this technique in crustacean research is somewhat hampered by the current limitations of crustacean proteome databases. These databases are crucial, as the identification of neuropeptides typically involves comparing the mass spectra of unknown peptides with the predicted spectra of known peptides, which are derived from these databases.…”

Section: Introductionmentioning

confidence: 99%

Neuropeptidomics of the American Lobster Homarus americanus

Lu,

Tran,

et al. 2024

J. Proteome Res.

Self Cite

View full text Add to dashboard Cite

The American lobster, Homarus americanus, is not only of considerable economic importance but has also emerged as a premier model organism in neuroscience research. Neuropeptides, an important class of cell-to-cell signaling molecules, play crucial roles in a wide array of physiological and psychological processes. Leveraging the recently sequenced high-quality draft genome of the American lobster, our study sought to profile the neuropeptidome of this model organism. Employing advanced mass spectrometry techniques, we identified 24 neuropeptide precursors and 101 unique mature neuropeptides in Homarus americanus. Intriguingly, 67 of these neuropeptides were discovered for the first time. Our findings provide a comprehensive overview of the peptidomic attributes of the lobster's nervous system and highlight the tissue-specific distribution of these neuropeptides. Collectively, this research not only enriches our understanding of the neuronal complexities of the American lobster but also lays a foundation for future investigations into the functional roles that these peptides play in crustacean species. The mass spectrometry data have been deposited in the PRIDE repository with the identifier PXD047230.

show abstract

Peptidomics

Cited by 41 publications

References 274 publications

Parahydrogen hyperpolarized NMR detection of underivatized short oligopeptides

Parahydrogen hyperpolarized NMR detection of underivatized short oligopeptides

Cyclic Peptides with Antifungal Properties Derived from Bacteria, Fungi, Natural Products, and Synthetic Sources

Neuropeptidomics of the American Lobster Homarus americanus

Contact Info

Product

Resources

About