Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats

Arita, Seiichi; Hatta, Mitsutoki; Uchida, Kunitoshi; Kita, Tomo; Okamura, Kazuhiko; Ryu, Takanori; Murakami, Hiroshi; Sakagami, Ryuji; Yamazaki, Jun

doi:10.1111/jre.12561

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…Massive deimination of filaggrin is favorable to its complete proteolytic breakdown, leading to the production of free amino acid derivatives, a key process for the maintenance of tissue hydration [33]. We also identified a chemical activator of PADs, aceffyline, a xanthine derived from caffeine that promotes the deimination of filaggrin and consequently cornified epithelium hydration [100,101], as also observed by others [102]. Filaggrin is a basic protein that interacts with keratin IF in the upper epidermis to promote their aggregation [103].…”

Section: Deimination Of Ifap Impact On Their Proteolysissupporting

confidence: 63%

Deimination, Intermediate Filaments and Associated Proteins

Briot

Simon

Méchin

2020

IJMS

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Deimination (or citrullination) is a post-translational modification catalyzed by a calcium-dependent enzyme family of five peptidylarginine deiminases (PADs). Deimination is involved in physiological processes (cell differentiation, embryogenesis, innate and adaptive immunity, etc.) and in autoimmune diseases (rheumatoid arthritis, multiple sclerosis and lupus), cancers and neurodegenerative diseases. Intermediate filaments (IF) and associated proteins (IFAP) are major substrates of PADs. Here, we focus on the effects of deimination on the polymerization and solubility properties of IF proteins and on the proteolysis and cross-linking of IFAP, to finally expose some features of interest and some limitations of citrullinomes.

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Section: Deimination Of Ifap Impact On Their Proteolysissupporting

confidence: 63%

Deimination, Intermediate Filaments and Associated Proteins

Briot

Simon

Méchin

2020

IJMS

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“…With respect to the role of citrullination in the oral mucosa, Arita et al reported that PAD contributes to the integrity of the stratum corneum of the oral mucosa by citrullinating filaggrin and profilaggrin in the rat palate [ 32 ]. In addition to filaggrin, other citrullinated peptides with physiological roles include the glial cell fibrillary acidic protein [ 33 ], myelin basic protein [ 34 ], and histones [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of salivary citrullinated cytokeratin 13 in healthy individuals and patients with rheumatoid arthritis by proteomics analysis

2022

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The immune response to citrullinated peptides in the mucosa has been suggested to play an important role in the transition from pre-onset rheumatoid arthritis (RA) to clinically evident RA. Although there are reports indicating the presence of anti-citrullinated peptide antibodies in the saliva, few studies have reported citrullinated peptide detection in human saliva. This study aimed to identify citrullinated peptides in human saliva and discuss their clinical significance. Saliva samples were collected from 11 patients with RA and from 20 healthy individuals. Citrullinated peptides were detected using an anti-modified citrulline (AMC) antibody. Saliva from the healthy individuals was subjected to two-dimensional protein electrophoresis to isolate citrullinated peptides, which were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and mass spectrometry by peptide mass fingerprinting. The results were corroborated by immunoprecipitation (IP)-western blotting. The signal intensities of the bands precipitated with anti-cytokeratin 13 (CK13) and AMC antibodies were quantified. The signal intensity ratio of the band produced by the AMC antibody was divided by that of the band produced by the anti-CK13 antibody to calculate the citrullinated CK13 (Cit-CK13) ratio. A citrullinated peptide band corresponding to a molecular weight of approximately 50 kDa was detected in the saliva of healthy individuals, and identified as CK13 via mass spectrometry and IP-western blotting. No significant difference was observed between the salivary Cit-CK13 ratios of patients with RA and healthy participants (p = 0.605). This is the first study to show that Cit-CK13 is present in human saliva, and that there is no significant difference between the Cit-CK13 ratios of patients with RA and healthy individuals, suggesting that salivary Cit-CK13 content and RA development may not be associated. The physiological and pathological roles of Cit-CK13 in the oral cavity, and its responsiveness to mucosal immunity, remain unknown and will be the subject of further investigation.

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“…Fifty percent inhibition of PADs using chloro-amidine, a pan-PAD inhibitor non-toxic for keratinocytes, partly reversed this consequence of dryness on keratinocyte metabolism [42]. Similarly, inhibition of PADs in an organotypic model, derived from rat palate keratinocytes reduced the breakdown of FLG and increased its association with keratins [43]. In this experimental model, activation of PADs with acefylline, a PAD1 and PAD3 activator [44], had the opposite effects.…”

Section: Pads and Deiminated Proteins In The Epidermismentioning

confidence: 92%

Deimination and Peptidylarginine Deiminases in Skin Physiology and Diseases

Méchin

Takahara

Simon

2020

IJMS

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Deimination, also known as citrullination, corresponds to the conversion of the amino acid arginine, within a peptide sequence, into the non-standard amino acid citrulline. This post-translational modification is catalyzed by a family of calcium-dependent enzymes called peptidylarginine deiminases (PADs). Deimination is implicated in a growing number of physiological processes (innate and adaptive immunity, gene regulation, embryonic development, etc.) and concerns several human diseases (rheumatoid arthritis, neurodegenerative diseases, female infertility, cancer, etc.). Here, we update the involvement of PADs in both the homeostasis of skin and skin diseases. We particularly focus on keratinocyte differentiation and the epidermal barrier function, and on hair follicles. Indeed, alteration of PAD activity in the hair shaft is responsible for two hair disorders, the uncombable hair syndrome and a particular form of inflammatory scarring alopecia, mainly affecting women of African ancestry.

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Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats

Abstract: Citrullination is likely a protein modification that plays an important role in maintaining the structure and function of oral cornified mucosa in a way that is distinctly different from that of the skin.

Cited by 4 publications

References 38 publications

Deimination, Intermediate Filaments and Associated Proteins

Deimination, Intermediate Filaments and Associated Proteins

Detection of salivary citrullinated cytokeratin 13 in healthy individuals and patients with rheumatoid arthritis by proteomics analysis

Deimination and Peptidylarginine Deiminases in Skin Physiology and Diseases

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