Peptoniphilus stercorisuis sp. nov., isolated from a swine manure storage tank and description of Peptoniphilaceae fam. nov.

Abstract

Peptoniphilus stercorisuis sp. nov., isolated from a swine manure storage tank and description of Peptoniphilaceae fam. nov. ). Our studies demonstrate that the genus Peptoniphilus shares a close relationship with the genera Anaerococcus, Anaerosphaera, Helcococcus, Finegoldia, Gallicola, Murdochiella and Parvimonas and that these organisms share consistent biochemical and chemotaxonomic traits which support the proposal to create a family to embrace these genera. In this study, we report the isolation and characterization of a novel species recovered from stored swine manure. The production of odorous chemicals that include ammonia, organic acids and alcohols, and sulphides by micro-organisms, and an understanding of the underlying processes of the productionThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SF-S1 T is KF705042. of these compounds is the focus of ongoing research on swine manure management (Miller, 2001). The Grampositive, obligately anaerobic, coccal-shaped organism isolated from stored swine manure was characterized using biochemical, chemotaxonomic and phylogenetic methods, and based upon these findings presented here, a novel species of the genus Peptoniphilus is proposed.Strain SF-S1 T was isolated from a manure storage tank located at a pig production facility in central Oklahoma, USA. A sample of pig slurry was collected from a 3.0 m depth using a tank sampler (NASCO); the environmental conditions of the sample site were 28 u C, pH 7.3 and when allowed to settle, the particulate matter was found to be approximately 50 % (v/v). The sample was transferred to a nitrogen-flushed sterile bottle that was then sealed to maintain an anaerobic environment, and transported back to the laboratory on ice. A 10 % inoculum was used for enrichment in Brain Heart Infusion (BHI; Oxoid) broth at pH 7.0 and was incubated at room temperature with H 2 :N 2 :CO 2 headspace (5 : 10 : 85) at 20 p.s.i. Isolation was achieved through serial plate dilutions and sub-cultures on Tryptic Soy Agar (Becton, Dickinson and Company) amended with 5 % defibrinated sheep blood, and incubated in anoxic jars containing H 2 :N 2 :CO 2 headspace (5 : 10 : 85) at 37 u C. Colonies were picked and sub-cultured onto fresh media until well-isolated. For phenotypic analysis, cells that had been grown anaerobically for 5 days at 37 u C on Brucella blood agar (Oxoid) were used for all tests unless otherwise stated. Cells were examined with an Olympus CX41 microscope using phase-contrast at 61000 magnification. For biochemical characterization, conventional tests and Rapid ID 32A and API ZYM test systems (bioMérieux) were employed with all tests performed in duplicate (Jousimies-Somer et al., 2002;Tindall et al., 2007). For additional tests, except where stated, the strain was incubated in Hungate tubes containing anoxic peptoneyeast extract (PY) medium (Holdeman et al., 1977) supplemented with 0.1 % cysteine sulfide. Fermentation tests were performed using fructose, mannose, glucose, sucrose, xylose and cell...

Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.

“…The fatty acid composition of the three strains was as following: strain KhD‐2 T contained saturated acid C 16:0 (41.6%) and C 14:0 (14.7%); unsaturated acids were also detected (Table ); strains KHD4 T and Kh‐D5 T contained C 16:0 (32% and 36%, respectively), C 18:2ω6 (26% and 24%, respectively), and C 18:1ω9 (26% and 21%, respectively) (Table ). These fatty acid results were likened to those of related species in Table (Johnson et al., ; Rooney, Swezey, Pukall, Schumann, & Spring, ). Strain KhD‐2 T can be distinguished from its nearest neighbor P. harei by the production of C 14:0 (14.7% vs. 4.4%).…”

“…These bacteria exhibited phylogenetic and phenotypic proximity to species of the Peptoniphilus genus. Created after the division of Peptostreptococcus genus into five genera (Ezaki et al., ), the Peptoniphilus genus belonging to the Peptoniphilaceae family that regroup members of the genera Peptoniphilus , Parvimonas, Murdochiella , Helcococcus , Gallicola , Finegoldia, Ezakiella , Anaerosphaera , and Anaerococcus (Johnson, Whitehead, Cotta, Rhoades, & Lawson, ; Patel et al., ). The Peptoniphilus genus is currently made of 16 valid published species (http://www.bacterio.net/peptoniphilus.html).…”

‘ Khoudiadiopia massiliensis ’ gen. nov., sp. nov., strain Marseille-P2746T T , a new bacterial genus isolated from the female genital tract

“…1). This value was lower than the 95% 16S rRNA gene sequence threshold proposed by Stackebrandt and Ebers [3] to define a new genus without carrying out DNA–DNA hybridization and classifies it as a new genus within the Peptoniphilaceae family (phylum Firmicutes ), first created in 2014 [4]. Murdochiella asaccharolytica is an obligate anaerobic species isolated from a sacro-pilonidal cyst aspirate from an immunocompetent patient.…”

Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru

“…1). These genera, Peptoniphilus, Anaerococcus , Anaerosphaera, Finegoldia , Gallicola, Helcococcus , Murdochiella and Parvimonas have recently been assigned to a newly described family Peptoniphilaceae [19]. The pairwise comparisons showed that strain M6.X2 T formed a loose cluster with the genera Finegoldia (86.7% sequence similarity), Gallicola (83.5% sequence similarity) and Parvimonas (84.4% sequence similarity).…”

“…Until recently the exact relationship of this group of organisms with other close close members of Firmicutes was somewhat uncertain, and was reflected in their placement in the Family XI Incertae Sedis (order Clostridiales , class Clostridia , phylum Firmicutes ) in the current edition of Bergey's Manual of Systematic Bacteriology [17,18]. However, Johnson et al, (2014)[19] recently described the family Peptoniphilaceae to accommodate the genera Peptoniphilus , Anaerococcus , Anaerosphaera , Finegoldia , Gallicola , Helcococcus , Murdochiella , and Parvimonas .…”