Perbanyakan In Vitro Dendrobium Indonesia Raya ‘Ina’  melalui Embriogenesis Somatik Berbasis Sistem Bioreaktor

Rachmawati, Fitri; Wiendi, Ni Made Armini; Mattjik, Nurhajati Ansori; Purwito, Agus; Winarto, Budi

doi:10.24831/jai.v44i3.12816

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…For instance, clone NS 001-31 had better organogenesis ability compared to clone NS 022/62, which in turn had a higher ability to form EC than eight of the other clones (Rachmawati et al 2010). D. Indonesia Raya Ina had the best response to initiation and proliferation of EC compared to D. Sonia Earsakul and D. Gradita 10 with 89.1% to 99.7% regeneration of explants and 87.5% to 98.7% formation of EC (Rachmawati et al 2016b). Additionally, D. CF22-58 produced a higher EC proliferation improvement up to 356.7% with a 4.6 multiplication rate than D. Dian Agrihorti (Rachmawati et al, 2022).…”

Section: Discussion Discussion Discussionmentioning

confidence: 88%

“…Orbital shaker was used as culture system for D. Gradita 31, and D. Indonesia Raya (Rachmawati et al, 2015). The combination of shoot tips explant, half strength MS medium, and airlift bioreactor were applied for D. Zahra FR 62, D. Gradita 10, D. Indonesia Raya Ina, and D. Balithi CF22-58 Rachmawati et al, 2014Rachmawati et al, , 2016aRachmawati et al, , 2019Rachmawati et al, , 2022. While, the combination of basal part of plantlets as an explant, half strength MS medium, and airlift bioreactor for D. lasianthera x D. antennatum (Sasmita et al, 2022).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

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Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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The study focuses on two Indonesia Dendrobium varieties, D. Dian Agrihorti (DDA) and D. Syifa Agrihorti (DSA), which have potential to be commercialized, but face limitations on the availability of qualified seedlings sustainably. The research aimed to establish an in vitro propagation protocol using a Temporary Immersion System (TIS) to produce high-quality seedlings efficiently. Various factors, including varieties, media, plant growth regulators, anti-phenol compounds, organic additives, and TIS settings, were investigated using the basal part of the plantlet as the explant source. Key findings revealed that DDA outperformed DSA across all observed variables. In the initiation phase, basal plantlets cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 thidiazuron and 0.5 mg L-1 N-6 benzylaminopurine enhanced embryogenic callus (EC) formation, with a 13.5-day initiation period, 72% potential explant growth, 0.41 cm callus size, and a 3.45 rate of multiplication. During the proliferation stage, the addition of 150 mg L-1 ascorbic acid (AC) and the application of a TIS with a 30-minute dry period and a 15-minute wet period resulted in a 515.5% increase in EC fresh weight for DDA accompanied by a 6.16 multiplication rate. Regeneration of shoots was achieved using a Vacin and Went medium with 150 g L-1 banana extract, yielding 29.2 shoots per clump. Subsequent rooting of the shoots in 2 g L-1 Hyponex® medium with 20 g L-1 sugar and 2% AC proved successful. Acclimatization of plantlets with Cycas rumpii bulk demonstrated a 100% survivability rate. The established propagation protocol for DDA holds significant potential for application to other Dendrobium varieties, offering a sustainable and efficient method for meeting commercial demands in the Indonesian market.

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Section: Discussion Discussion Discussionmentioning

confidence: 88%

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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“…TDZ meningkatkan kerja sitokinin lain baik sitokinin eksogen maupun endogen (Restanto et al, 2018). TDZ dengan konsentrasi 0.3-1.5 mg L -1 dilaporkan efektif untuk pertunasan anggrek Dendrobium (Rachmawati et al, 2016). Tujuan penelitian ini untuk memperoleh konsentrasi kombinasi BA dan NAA yang terbaik dalam proses induksi kalus tanaman anggrek Dendrobium Hibrida (Dendrobium lasiantera x Dendrobium antennatum) melalui somatik embriogenesis.…”

Section: Pendahuluanunclassified

“…Media yang digunakan untuk proses proliferasi adalah media ½ MS (Murashige dan Skoog, 1962) yang dikombinasikan dengan penambahan thidiazuron (TDZ) dan naphthaleneacetic acid (NAA) agar dapat berproliferasi. Komposisi media proliferasi yang digunakan yaitu ½ media MS + 0.3 mg L -1 TDZ + 0.1 mg L -1 NAA (Rachmawati et al, 2016). Tahap proliferasi dilakukan selama 4-6 minggu pada ruang gelap dengan suhu ± 20 o C.…”

Section: Proliferasi Kalusunclassified

Somatic Embryogenesis of Dendrobium lasianthera X Dendrobium antennatum with the Addition of BA and NAA

Sasmita

Dewanti

Alfian³

2022

J. Agron. Indonesia

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Somatik embriogenesis merupakan teknik perbanyakan kultur in vitro melalui proses pembelahan sel yang berasal dari bagian tanaman untuk membentuk embrio menjadi tanaman baru. Perbanyakan tersebut didukung oleh penambahan BA dan NAA dalam media untuk memicu terbentuknya kalus. Tujuan penelitian ini untuk memperoleh konsentrasi kombinasi BA dan NAA yang terbaik dalam proses induksi kalus tanaman anggrek Dendrobium Hibrida (Dendrobium lasiantera x Dendrobium antennatum) melalui metode somatik embriogenesis. Penelitian ini dilakukan di Laboratorium Center for Development of Advance Sciences and Technology (CDAST) Universitas Jember Jawa Timur dari bulan Maret-September 2021. Rancangan penelitian yang digunakan yaitu rancangan acak lengkap dengan dua faktor yaitu faktor BA 4 taraf, 0, 0.025, 0.05, dan 0.1 mg L-1 dan faktor NAA 3 taraf; 1, 2, dan 3 mg L-1, dengan menggunakan tiga ulangan. Hasil penelitian menunjukkan waktu terbentuknya kalus anggrek Dendrobium Hibrida paling cepat pada perlakuan dengan konsentrasi BA 0.025 mg L-1 dan NAA 3 mg L-1 dengan presentase tertinggi terbentuknya kalus embriogenik yaitu sebesar 67%. Hasil penelitian menunjukkan bahwa penambahan BA 0.025 mg L-1 dan NAA 3 mg L-1 merupakan hasil terbaik pada variabel waktu terbentuknya kalus dan presentase kalus embriogenik.

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“…Pada penelitian ini juga diperoleh informasi bahwa penambahan 225 mg/l L-Cystein dan 150 mg/l Casein Hydrolisate menyebabkan pencokelatan yang cukup tinggi, yaitu mencapai 52%. Beberapa penelitian mengungkapkan aplikasi jenis asam amino tertentu dalam konsentrasi yang tepat memberikan pengaruh yang positif terhadap keberhasilan kultur, tetapi pada konsentrasi yang tidak sesuai dapat menimbulkan keracunan sel dan menghambat regenerasinya (Winarto et al 2016;Rachmawati et al 2016).…”

Section: Percobaan-2 : Pengaruh Jenis Dan Konsentrasi Asam Amino Terhadap Proliferasi Kalus Embriogenik Phal 'Raiza Agrihorti'unclassified

Pengaruh Jenis Eksplan dan Asam Amino pada Inisiasi dan Proliferasi Kalus Embriogenik Phalaenopsis Var. ‘Raiza Agrihorti’

Rachmawati¹,

Badriah²,

Marwoto³

2021

J.Hort

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(The Effect of Explant Types and Amino Acids on Embryogenic Callus Initiation and Proliferation of Phalaenopsis Var. ‘Raiza Agrihorti’)Penyiapan kalus embriogenik (KE) yang optimal memiliki peranan penting dalam menghasilkan benih bermutu Phalaenopsis skala komersial. Kendala utama yang dihadapi ialah inisiasi dan proliferasi KE yang masih rendah, serta akumulasi fenolik yang tinggi. Penelitian dilakukan di Laboratorium Kultur Jaringan Balithi dari Agustus 2019 hingga Juli 2020. Penelitian menggunakan Rancangan Acak Kelompok (RAK) pola split plot dan faktorial dengan lima ulangan. Percobaan-1: jenis eksplan (pucuk, pangkal, dan daun plantlet) sebagai petak utama dan perlakuan asam amino (tanpa asam amino, L-Proline, L-Glutamine, L-Cysteine, dan Casein-Hydrolisate) dengan konsentrasi 150 mg/l pada medium PC1 (1/2 MS + 1,0 mg/l TDZ + 0,5 mg/l BAP + 20 g/l sukrosa) sebagai anak petak. Percobaan-2: faktor-1 ialah jenis asam amino (L-Proline, L-Cysteine; L-Glutamine, dan Casein-Hydrolisate) dan faktor-2 ialah konsentrasi asam amino (0, 75, 150, 225, dan 300 mg/l). Hasil penelitian menunjukkan bahwa inisiasi KE Phalaenopsis var. ‘Raiza Agrihorti’ terbaik didapatkan dari pangkal plantlet dan 150 mg/l L-Glutamine dengan waktu inisiasi 18,3-24,0 hari, 80-100% pembentukan KE, dan ukuran KE 0,4-0,5 cm3. Proliferasi KE terbaik ditemukan pada L-Glutamine dengan konsentrasi 150 mg/l. Proliferasi KE mencapai 100% dengan penambahan berat segar sebesar 0,39 g, tingkat multiplikasi (MR) 4,55 kali dan pencokelatan 4,0%. Hasil penelitian ini berpotensi tinggi untuk diterapkan pada kultur starter Phalaenopsis hibrida lain.KeywordsPhalaenopsis hibrid; Asam amino; Inisiasi; Kalus embriogenik; ProliferasiAbstractSetup of the optimum Phalaenopsis embryogenic callus (EC) is an important role in producing qualified-seedlings of Phalaenopsis in commercial scale. The main constraints that are still being faced are the low rate of culture proliferation and high phenolic accumulations. The research was carried out at the Tissue Culture Laboratory-Indonesian Ornamental Plants Research Institite, from August 2019 through July 2020. The split plot and factorial designs were arranged using a Randomized Completely Block Design (RCBD) with five replications. Experiment-1: explants type (shoot tip, basal part, and leaf of plantlet) was used as main plot and amino acids (amino acids free, L-Proline, L-Glutamine, L-Cysteine, and Casein-Hydrolisate) with 150 mg/l concentration on medium PC1 (1/2 MS + 1,0 mg/l TDZ + 0,5 mg/l BAP + 20 g/l sukrosa) as subplot. Experiment-2: the first factor was amino acids type (L-Proline, L-Cysteine; L-Glutamine, and Casein-Hydrolisate) and the second factor was amino acids concentration (0, 75, 150, 225, and 300 mg/l). Results of the studies revealed that the best EC initiation of Phalaenopsis var. ‘Raiza Agrihorti’ was produced by basal part of plantlet and PC1 medium containing 150 mg/l L-Glutamine with EC Initiation time was 18.3-24.0 days, 80-100% of EC formation and size of 0.4-0.5 cm3. The best proliferation of EC was found in L-Glutamine with 150 mg/l concentration. EC proliferation reached 100% with 4.55 EC multiplication rate, 0.39 g EC fresh weight added, and EC browning as low as 4.0%. The established method is high possibly applied for other Phalaenopsis hybrids.

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Perbanyakan In Vitro Dendrobium Indonesia Raya ‘Ina’ melalui Embriogenesis Somatik Berbasis Sistem Bioreaktor

Cited by 4 publications

References 14 publications

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

Somatic Embryogenesis of Dendrobium lasianthera X Dendrobium antennatum with the Addition of BA and NAA

Pengaruh Jenis Eksplan dan Asam Amino pada Inisiasi dan Proliferasi Kalus Embriogenik Phalaenopsis Var. ‘Raiza Agrihorti’

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