Perbedaan Kualitas Spermatozoa Mencit Jantan (Mus Musculus L) Yang Diberikan Vitamin C  Setelah Pemaparan Asap Rokok

Claudia, Vergina; Queljoe, Edwin De; Tendean, Lydia

doi:10.35790/ebm.1.1.2013.4610

Cited by 6 publications

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“…Other research, which administer only vitamin C to male rats (Mus musculus L) after cigarette smoke exposure, showed that vitamin C can improve sperm quality. 6 In vitro study proved that vitamin E can protect sperm from damage caused by oxidative stress, loss of motility, and morphological changes. 22 In conclusion, combination of vitamin C and E can improve sperm concentration, motility, and morphology, and also decrease cement 8-OHdG levels of smoke exposed rats.…”

Section: Discussionmentioning

confidence: 99%

“…5 Researches on the role of vitamin C in cigarette smoke exposed rats showed positive effect for the quality of spermatozoa and the mean score of spermatogenesis. [6][7] Studies of vitamin E showed positive effect on spermatogenesis and sperm function in rats who suffered from spinal cord injuries. [8][9] Until now, there has not been any experiment which suggest that combination between vitamin C and vitamin E can provide protective effects on the fertility of smokers with sperm quality and levels of cement 8-hydroxy-2'-deoxyguanosine (8-OHdG) as indicators.…”

Section: Introductionmentioning

confidence: 99%

“…1,12,13 Other studies showed the occurrence of sperm DNA damage, such as a marked increase in 8-OhdG level; a parameter of DNA damage caused by oxidative stress, which can be caused by cigarettes. 2,3,14 Research by Claudia et al, 6 concluded that vitamin C can improve sperm quality (motility, concentration and morphology) of male rats exposed by cigarette smoke. Research by Intania 7 also showed that vitamin C increased the average score of spermatogenesis in male rats exposed to cigarette smoke.…”

Section: Introductionmentioning

confidence: 99%

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The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

et al. 2017

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Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

et al. 2017

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“…Bagian badan spermatozoa mengandung mitokondria yang berfungsi memproduksi ATP untuk pergerakan bagian ekor. Gerakan ekor yang bergelombang mengakibatkan motilitas spermatozoa untuk berenang ( Claudia, De Queljoe, & Tendean, 2013).…”

Section: Hasilunclassified

EFEK KONSUMSI KERANG BULU (Anadara antiquata) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA

Safitri

Hanizar

2019

Al-Kauniyah J. Biol.

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Kerang Bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi seperti protein, lemak, vitamin dan mineral yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek kerang bulu (A.antiquata) terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial, faktor pertama kondisi kerang yakni mentah dan kukus, faktor kedua konsentrasi 0%(kontrol), 75% dan 100%. M. musculus yang digunakan sebanyak 25 ekor terdiri dari limAbstrakKerang bulu (Anadara antiquata) merupakan salah satu makanan yang mengandung nutrisi tinggi, seperti protein, lemak, vitamin, dan mineral, yang berpotensi memperbaiki serta meningkatkan kualitas spermatozoa. Penelitian ini bertujuan untuk menganalisis efek A. antiquata terhadap kuantitas dan kualitas spermatozoa Mus musculus. Penelitian ini merupakan penelitian eksperimental murni dengan rancangan acak lengkap pola faktorial. Faktor pertama adalah kondisi kerang, yakni mentah dan kukus, dan faktor kedua adalah konsentrasi 0 g/mL, 75 g/100 mL, dan 100 g/100 mL. M. musculus yang digunakan sebanyak 25 ekor, terdiri dari lima perlakuan dengan masing-masing lima kali ulangan. Parameter yang diamati adalah konsentrasi (kuantitas), motilitas, dan morfologi (kualitas) spermatozoa. Data dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan bahwa A. antiquata berpengaruh nyata terhadap konsentrasi dan motilitas spermatozoa, sedangkan morfologi tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100 g/100 mL memberikan efek rata-rata terendah terhadap konsentrasi dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75 g/100 mL meningkatkan rata-rata konsentrasi dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan bahwa mengonsumsi kerang kukus lebih baik untuk meningkatkan konsentrasi dan motilitas spermatozoa dibandingkan dalam kondisi mentah. Peningkatan dosis kerang kukus tidak linier terhadap respon kuantitas dan kualitas spermatozoa.Kata kunci: Anadara antiquata; Konsentrasi spermatozoa; Motilitas; Morfologi; Mus musculusAbstract Shellfish (Anadara antiquata) was one of the foods that contained high nutrients such as protein, fat, vitamins, and minerals that had the potential to improve and increase the quality of sperm. The study aims to analyze the effect of the shellfish on the quantity and quality of Mus musculus sperm. This study was a purely experimental study with a completely randomized factorial pattern; the first factor was the condition of shellfish which was raw and steamed, and the second factor was concentrations of 0 g/mL, 75 g/100 mL and 100 g/100 mL. Twenty-five individuals of M. musculus were subject to five treatments, each with five replications. Parameters observed were concentration, motility, and morphology of sperm. Data were analyzed using the Kruskal-Wallis test and Duncan's advanced test. The results showed that shellfish had a significant effect on the concentration and motility of sperm, whereas there was no significant effect on morphology. The dose which gave the effect was 100 g/100 mL of raw shellfish and 75 g/100 mL of steamed shellfish. This study concluded that consuming steamed shellfish was better than raw for increasing the concentration and motility of sperm. The shellfish dose increased is not linear in response to the quantity and quality of sperm.a perlakuan masing-masing lima kali ulangan. Parameter yang diamati jumlah (kuantitas), motilitas dan morfologi (kualitas) spermatozoa. Data dinalisis menggunakan uji Kruskal-wallis dan uji lanjut Duncan. Hasil penelitian menunjukkan kerang bulu (A.antiquata ) pengaruh nyata terhadap jumlah dan motilitas spermatozoa sedangkan pada morfologi tidak memberikan pengaruh yang nyata. Kondisi kerang mentah dengan konsentrasi 100g/100 ml air memberikan efek rata-rata terendah terhadap jumlah dan motilitas spermatozoa, sementara kerang kukus dengan konsentrasi 75g/100 ml meningkatkan rata-rata jumlah dan motilitas spermatozoa tertinggi. Penelitian ini memberi kesimpulan mengkonsumsi kerang kukus lebih baik untuk meningkatkan jumlah dan motilitas spermatozoa dibanding dalam kondisi mentah. Peningkatan dosis tidak linier terhadap respon kuantitas dan kualitas spermatozoa.

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“…The dosage requirements for males are higher than for females (German Nutrition Society, 2015). In animals, vitamin C has been shown to increase sperm count and motility of rats after being given the stress of swimming (Vijayprasaad, 2014) or exposed the cigarette smoke (Claudia, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Lemon (Citrus limon) Extracts On The Quantity and Quality of Mice (Mus musculus) Sperm

Rahayu

Hanizar

2022

EKW

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Vitamin C has been proved as a nutrient to improve the quality of sperm. Society believed that consuming the lemons could potentially enhance the sperm quality of humans. However, the appropriate concentration should be well studied to obtain the optimum concentration to improve the sperm quality and quantity. The present research tried to provide information on how lemon could improve the sperm quality by designing a true experimental using a series concentration of lemon extract (25%, 50% and 75 % concentrations) given to the male mice (Mus musculus). The investigation was made by giving the lemon treatment three times a day for 5 weeks. To investigate the effect of lemon extract, the mice sperm were taken from the epididymis and observed using a multimedia microscope and counted using Neubauer’s counting rooms, while motility and morphology were observed using object-glass. The result showed that the high concentration of lemon could not provide the greatest improvement of sperm quality and quantity. The optimum condition was seen in 25% of lemon extract, where the increase of lemon concentration suppressed the lemon improvement effect, which reduced the sperm quality and quantity. However, the improvement was still made if the result was compared to control, meaning consuming lemon was better than consuming any lemon treatment. The result was in accordance with quality improvement of sperm, where 25% of lemon concentration treatment provided the highest average motility and normal morphology of sperm. However, the high concentration of lemon extract (50% and 75% of lemon concentrations) provided a lower effect due to the adverse effect. The result proved that lemon could be used to boost the quality and quantity of sperm in an appropriate concentration where the excess lemon extract could reduce the effect of lemon in improving sperm quality and quantity.Abstrak: Vitamin C telah terbukti sebagai nutrisi untuk meningkatkan kualitas sperma dimana masyarakat percaya bahwa mengkonsumsi lemon berpotensi meningkatkan kualitas sperma manusia. Namun, konsentrasi yang tepat harus dipelajari dengan baik untuk mendapatkan konsentrasi yang optimal untuk meningkatkan kualitas dan kuantitas sperma. Penelitian ini mencoba memberikan informasi bagaimana lemon dapat meningkatkan kualitas sperma dengan merancang eksperimen nyata menggunakan serangkaian konsentrasi ekstrak lemon (konsentrasi 25%, 50% dan 75%) yang diberikan kepada mencit jantan (Mus musculus). Penyelidikan dilakukan dengan memberikan ekstrak lemon sebanyak tiga kali dalam sehari selama 5 minggu. Untuk mengetahui pengaruh ekstrak lemon, sperma mencit diambil dari epididimis dan diamati menggunakan mikroskop multimedia dan dihitung menggunakan kamar hitung Neubauer, sedangkan motilitas dan morfologi diamati menggunakan kaca objek. Hasil penelitian menunjukkan bahwa konsentrasi lemon yang tinggi tidak dapat memberikan peningkatan kualitas dan kuantitas sperma yang tertinggi. Kondisi optimum terlihat pada ekstrak lemon 25% dimana peningkatan konsentrasi lemon menekan efek perbaikan lemon yang menurunkan kualitas dan kuantitas sperma. Namun perbaikan tetap dilakukan jika hasilnya dibandingkan dengan kontrol, artinya mengkonsumsi lemon lebih baik daripada tanpa mengkonsumsi lemon. Hasil tersebut sesuai dengan peningkatan kualitas sperma dimana perlakuan konsentrasi lemon 25% memberikan rata-rata motilitas dan morfologi sperma yang normal paling tinggi. Namun, konsentrasi tinggi ekstrak lemon (50% dan 75% konsentrasi lemon) memberikan efek yang lebih rendah karena efek samping. Hasil penelitian membuktikan bahwa lemon dapat digunakan untuk meningkatkan kualitas dan kuantitas sperma dalam konsentrasi yang sesuai dimana kelebihan ekstrak lemon dapat mengurangi efek lemon dalam meningkatkan kualitas dan kuantitas sperma.

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Perbedaan Kualitas Spermatozoa Mencit Jantan (Mus Musculus L) Yang Diberikan Vitamin C Setelah Pemaparan Asap Rokok

Cited by 6 publications

References 0 publications

The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

EFEK KONSUMSI KERANG BULU (Anadara antiquata) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA

The Effect of Lemon (Citrus limon) Extracts On The Quantity and Quality of Mice (Mus musculus) Sperm

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