Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells

Capo-chichi, Callinice D.; Rula, Malgorzata E.; Smedberg, Jennifer L.; Vanderveer, Lisa; Parmacek, Michael S.; Morrisey, Edward E.; Godwin, Andrew K.; Xu, Xiang Xi

doi:10.1016/j.ydbio.2005.07.037

Cited by 93 publications

(142 citation statements)

References 40 publications

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“…Differentiation of mESCs into extraembryonic endoderm (ExE) was carried out by treatment with retinoic acid as previously described. 29 Cell populations of mESCs, EBs, and ExE were characterized by flow cytometry using the lineage-specific marker CD9 and by transcript analysis using quantitative RT-PCR. RNA was prepared using Qiagen RNeasy Mini Kits.…”

Section: Methodsmentioning

confidence: 99%

Glycomics of Proteoglycan Biosynthesis in Murine Embryonic Stem Cell Differentiation

et al. 2007

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Glycosaminoglycans (GAGs) play a critical role in binding and activation of growth factors involved in cell signaling critical for developmental biology. The biosynthetic pathways for GAGs have been elucidated over the past decade and now analytical methodology makes it possible to determine GAG composition in as few as 10 million cells. A glycomics approach was used to examine GAG content, composition, and the level of transcripts encoding for GAG biosynthetic enzymes as murine embryonic stem cells (mESCs) differentiate to embryoid bodies (EBs) and to extraembryonic endodermal cells (ExE) to better understand the role of GAGs in stem cell differentiation. Hyaluronan synthesis was enhanced by 13-and 24-fold, most likely due to increased expression of hyaluronan synthase-2. Chondroitin sulfate (CS)/dermatan sulfate (DS) synthesis was enhanced by 4-and 6-fold, and heparan sulfate (HS) synthesis was enhanced by 5-and 8-fold following the transition from mESC to EB and ExE. Transcripts associated with the synthesis of the early precursors were largely unaltered, suggesting other factors account for enhanced GAG synthesis. The composition of both CS/DS and HS also changed upon differentiation. Interestingly, CS type E and highly sulfated HS both increase as mESCs differentiate to EBs and ExE. Differentiation was also accompanied by enhanced 2-sulfation in both CS/DS and HS families. Transcript levels for core proteins generally showed increases or remained constant upon mESC differentiation. Finally, transcripts encoding selected enzymes and isoforms, including GlcNAc-4,6-O-sulfotransferase, C5-epimerases, and 3-O-sulfotransferases involved in late GAG biosynthesis, were also enriched. These biosynthetic enzymes are particularly important in introducing GAG fine structure, essential for intercellular communication, cell adhesion, and outside-in signaling. Knowing the changes in GAG fine structure should improve our understanding the biological properties of differentiated stem cells.

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Section: Methodsmentioning

confidence: 99%

Glycomics of Proteoglycan Biosynthesis in Murine Embryonic Stem Cell Differentiation

et al. 2007

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“…Chemical induced differentiation has since been reported for human embryonal carcinoma (EC) cells [145] and for human and mouse embryonic stem (ES) cells [146][147][148][149][150]. One of these specific chemicals is RA, which is indispensable for embryonic development [2,151] and depending on the context is considered an inducer, a morphogen and/or a teratogen to developing embryos.…”

Section: Definitive Endoderm Vs the Extraembryonic Endoderm Lineagementioning

confidence: 99%

Retinoic Acid and the Development of the Endoderm

Kelly

Drysdale

2015

JDB

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Retinoic acid (RA) is an important signaling molecule in the development of the endoderm and an important molecule in protocols used to generate endodermal cell types from stem cells. In this review, we describe the RA signaling pathway and its role in the patterning and specification of the extra embryonic endoderm and different endodermal organs. The formation of endoderm is an ancient evolutionary feature and RA signaling appears to have coevolved with the vertebrate lineage. Towards that end, we describe how RA participates in many regulatory networks required for the formation of extraembryonic structures as well as the organs of the embryo proper.

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“…To test this hypothesis and assess the capacity of RARRES3 to engage differentiation, we used a pluripotent embryonic mouse cell line, RW-4, where differentiation can be easily monitored. Treatment of RW-4 cells with alltrans retinoic acid (ATRA) induces differentiation, a process that is controlled by GATA6, since its absence precludes differentiation (Capo-Chichi et al, 2005). Thus, GATA6 controls and also can be used as a marker of the differentiation status in these cells (CapoChichi et al, 2005).…”

Section: Embo Molecular Medicinementioning

confidence: 99%

RARRES3 suppresses breast cancer lung metastasis by regulating adhesion and differentiation

Lahuerta¹,

Cabré²,

M³

2016

European Journal of Cancer

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In estrogen receptor-negative breast cancer patients, metastatic relapse usually occurs in the lung and is responsible for the fatal outcome of the disease. Thus, a better understanding of the biology of metastasis is needed. In particular, biomarkers to identify patients that are at risk of lung metastasis could open the avenue for new therapeutic opportunities. Here we characterize the biological activity of RARRES3, a new metastasis suppressor gene whose reduced expression in the primary breast tumors identifies a subgroup of patients more likely to develop lung metastasis. We show that RARRES3 downregulation engages metastasis-initiating capabilities by facilitating adhesion of the tumor cells to the lung parenchyma. In addition, impaired tumor cell differentiation due to the loss of RARRES3 phospholipase A1/A2 activity also contributes to lung metastasis. Our results establish RARRES3 downregulation as a potential biomarker to identify patients at high risk of lung metastasis who might benefit from a differentiation treatment in the adjuvant programme.

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Perception of differentiation cues by GATA factors in primitive endoderm lineage determination of mouse embryonic stem cells

Cited by 93 publications

References 40 publications

Glycomics of Proteoglycan Biosynthesis in Murine Embryonic Stem Cell Differentiation

Glycomics of Proteoglycan Biosynthesis in Murine Embryonic Stem Cell Differentiation

Retinoic Acid and the Development of the Endoderm

RARRES3 suppresses breast cancer lung metastasis by regulating adhesion and differentiation

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