“…Frequency of application will also affect the delivered dose; although one large application usually results in the absorption of a higher dose, a single application may also have a greater toxicological potential compared to frequent, smaller doses (Wester et al 1977(Wester et al , 1980Wilson and Holland 1982). Occlusion and duration of contact can also increase the amount of applied chemical absorbed (Howes and Black 1976;Nakaue and Buhler 1976).…”