Objective: to analyze the evidence of validity of an instrument for the evaluation of competencies in a nursing team of a university hospital. Method: methodological study to investigate evidence of validity of the scale, with previously validated content, was sent to 978 technical-administrative employees in education who worked in the nursing team in a university hospital, and the non-parametric sample consisted of 143 employees. The scale consisted of 10 competencies and 25 measurable actions in four levels of competencies. The internal consistency of the instrument was analyzed by calculating Cronbach's alpha coefficient and construct validity by confirmatory factor analysis. Results: 53 nurses, 30 nursing technicians and 60 nursing assistants participated, totaling 143 professionals working in the hospital of a federal university in the state of São Paulo, Brazil. The confirmatory factor analysis of the instrument, based on the structure of 10 competencies, presented good adjustment indexes with χ²/GL=1.706, SRMR=0.071, RMSEA=0.070 and CFI=0.906, conferring construct validity. Cronbach's alpha was 0.924. Conclusion: through the statistical procedures adopted, it was concluded that the instrument has evidence of validity based on both the internal structure - construct, and internal consistency.