Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres

Sousa, Alessandra Tammy Hayakawa Ito de; Makino, Hérica; Bruno, Valentina; Cândido, Stéfhano Luís; Nogueira, Beatriz S.; Menezes, Isabela Godoy; Nakazato, Luciano; Dutra, Valéria

doi:10.1590/1678-4162-10599

Cited by 10 publications

(11 citation statements)

References 20 publications

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“…Antimicrobial resistance is a major threat to global public health. Despite being a natural phenomenon, the emergence and rapid dissemination of resistant bacteria are directly related to the inadequate use of antimicrobials, the unavailability of new drugs, the incorrect disposal of drugs and the inadequate treatment of effluents, generating serious clinical and economic consequences associated with increased morbidity and mortality of patients [ 1 ]. Therefore, antimicrobial resistance must be understood as a One Health issue involving epidemiological aspects related to humans, animals and the environment [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

Silva

Campana

Vasconcelos

et al. 2020

IJERPH

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The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, blaKPC-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only blaKPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.

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Section: Introductionmentioning

confidence: 99%

Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

Silva

Campana

Vasconcelos

et al. 2020

IJERPH

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“…Imipenem and amikacin showed 100% treatment efficiency, and gentamicin showed good treatment potential (Table 2). The literature also shows that Klebsiella pneumoniae not only reveals an increase in antibiotic resistance in patients with UTI, but also a high resistance in domestic and wild animals 13 . The data here show that coagulase-negative Staphylococci and Pseudomonas aeruginosa presented 8% and 14% resistance, respectively, to imipenem (Table 5).…”

Section: Discussionmentioning

confidence: 99%

Infecção do trato urinário: estudo de sensibilidade e resistência bacteriana em pacientes internados

Stinghel

Kreitlow

Benz³

et al. 2022

Rev. Med. (São Paulo)

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Infecção do Trato Urinário (ITU) é uma doença bacteriana que acomete principalmente o sexo feminino com maior prevalência em crianças e idosos. Os antibióticos possuem uma capacidade de inibir o crescimento bem como destruir as bactérias que causam a ITU. O uso de antibióticos sem condução do antibiograma tem aumentado o número de isolados resistentes. O foco deste estudo foi investigar a incidência e a resistência aos antibióticos das bactérias causadoras da ITU em hospitais de Colatina. Laudos da urocultura dos pacientes hospitalizados no período de 2015 a 2019 foram investigados. Faixa etária, sexo, agentes etiológicos mais freqüentes e resistência aos antibióticos foram analisados. A avaliação estatística foi realizada pelo programa Origin 8,0 a p<0,05. Os resultados mostraram maior incidência de ITU em crianças e idosos com acometimento maior em pacientes do sexo feminino (56%). Klebsiella pneumoniae (31%), Escherichia coli (29%), Enterococcus sp (20%), Pseudomonas aeruginosa (7%), Proteus (7%) e Stapylococcus coagulase negativa (6%) foram as bactérias mais prevalentes. Klebsiella pneumoniae mostrou-se resistente em 41% dos antibióticos administrados, Stapylococcus coagulase negativa 38%, Pseudomonas aeruginosa 30%, Proteus 29%, Escherichia coli 21% e Enterococcus sp 19%. Amicacina, gentamicina, imipenem e vancomicina foram os antibióticos mais eficazes para o tratamento da ITU. Pacientes internados em hospitais de Colatina com ITU apresentaram resistência bacteriana em torno de 40% para a maioria dos antibióticos administrados. Portanto faz-se necessário o uso do antibiograma para evitar o aumento da resistência bacteriana nos ambientes hospitalares de Colatina – ES.

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“…The rate of resistance to meropenem detected in free-living birds in this research should also be highlighted (14.5%). Several studies involving wild birds, free-living or not, as well as domestic birds, present lower rates of resistance to this drug or none at all [82,83,30,84,85]. However, a more relevant point is the fact that this drug is a high cost carbapenemic with restricted use to hospitals in Brazil.…”

Section: Discussionmentioning

confidence: 99%

Wild birds as reservoirs of multidrug-resistant enterobacteria in Mulungu, Brazil

Beleza

Maciel

Carreira

et al. 2021

Preprint

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Caatinga is a biome unique to Brazil that is degraded by anthropogenic actions, which lead to the loss of biodiversity putting many species at risk of extinction. The Ceará State is located in the Caatinga and has a rich avifauna comprised of 433 species including 13 species that are threatened with extinction, which are found in the Baturité Massif. The aim of this study was to investigate the frequency and diversity of enterobacteria in wild birds and to determine their susceptibility to antimicrobials. Cloacal swab samples were collected from 50 individuals of 28 different species, including the Ceara Gnatheter (Conopophaga cearae) and Red-necked Tanager (Tangara cyanocephala cearensis), which are classified as vulnerable (VU) by the Brazilian Ministry of the Environment. A total of 55 isolates belonging to 14 different species of Enterobacteriaceae were identified. Among these, Pantoea agglomerans and Escherichia coli were the most prevalent species with isolation rates of 36% and 26%, respectively. The highest rate of antimicrobial resistance found was to ampicillin (41.8%), followed by nalidixic Acid (36.3%) and amoxicillin associated with clavulanic acid (32.7%). The drugs with the best efficacy were tobramycin (96.4%), ciprofloxacin (92.6%) and tetracycline (90.9%). Multidrug resistance was observed in 23.5% of the analyzed strains. This research provides important information about the composition of the cloacal microbiota of wild birds in Mulungu, Brazil, as well as their health status. In addition, these results demonstrate that they harbor multidrug-resistant strains of Enterobacteriaceae.

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Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres

Cited by 10 publications

References 20 publications

Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil

Infecção do trato urinário: estudo de sensibilidade e resistência bacteriana em pacientes internados

Wild birds as reservoirs of multidrug-resistant enterobacteria in Mulungu, Brazil

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