Perfil de sensibilidade antimicrobiana e detecção do gene ISS pela reação em cadeia da polimerase na tipificação de Escherichia coli patogênica em codornas de corte sob inspeção sanitária

Abstract

ABSTRACT.-Abreu D.L.C., Franco R.M., Nascimento E.R., Pereira V. The pathogenicity of Escherichia coli strains is partially related to the expression of virulence factors genes, present in genetic elements called plasmids. APEC strains responsible for diseases in birds may present the iss gene which increases the resistance of E. coli strains to the lityc effect of the host's serum, besides resistance to several antimicrobials. This study was conduced in order to detect E. coli in tracheae of meat-type quails and to evaluate, by the presence of the iss gene and the profile of antimicrobial susceptibility, the pathogenic potential of the isolated samples for birds and humans. One hundred and eighty tracheae of quails were collected for detection of E. coli, antimicrobial sensitivity tests, and for polymerase chain reaction (PCR), for detection of iss gene. From the examined quails, 8.9 % (16/180) were positive for E. coli, from which 20 strains of this bacterium were obtained. Most of them were resistant to Tetracycline (16/20), followed by Ceftadizime (13/20) and Nalidixic-acid (12/20) and only one isolate was resistant to Amoxicillin. The detection of iss gene occurred in 55% (11/20) of the isolates, indicating that these strains had the potential to be pathogenic not only for quails, but also for other kinds of birds, other animals and even human beings that would be in contact with these E. coli isolates.

Tipagem molecular e resistência aos antimicrobianos em isolados de Escherichia coli de frangos de corte e de tratadores na Região Metropolitana de Curitiba, Paraná

“…Para as cefalosporinas de 3ª geração, Abreu et al (2010) reportaram 65% de resistência para a ceftazidima em isolados de E. coli patogê-nica de codornas sob inspeção sanitária. Nos demais estudos, estes antimicrobianos não compuseram o padrão dos testes de suscetibilidade utilizado, talvez por se tratarem de antimicrobianos de prescrição humana.…”

Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

“…According to KWON et al (2008), the high frequency of this virulence factor reveals the importance in the ability of this bacterium to evade the host's defenses, and even multiply and disseminate, favoring the development of the disease. Despite the smaller number of positive samples for the iss gene in the samples of this study, this gene can be considered a marker of virulence, with the iss gene cited as the most prevalent in strains from diseased birds (ROCHA et al, 2008;ABREU et al, 2010). Another gene identified in this work was tsh, the temperature-regulated adhesin .…”

Virulence Genes and Resistance Profile of Escherichia coli Isolated in Meat Meal Samples

“…This gene also showed high prevalence in comparative studies between APEC strains and isolates from healthy birds [16,31]. The gene iss, is one of the genes most searched in APEC strains, involved in mechanisms that allow the escape of the bacteria from the action of the complement system and the process of phagocytosis, during infection [1].…”

“…A total of 40 Escherichia coli isolates, stored at -20ºC in 20% Brain Heart Infusion (BHI) 1 , belonging to a biological sample bank of the Animal Diagnostic Laboratory -LADA, located in the state of Pernambuco, Brazil, were analyzed.…”