Performance Assessment and Improvement of a Spilt-and-Recombine Micromixer for Immunomagnetic Cell Sorting

Chaktranond, Chainarong; Fukagata, Koji; Kasagi, Nobuhide

doi:10.1299/jfst.3.1008

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…(7,8) They are also defined as a labeling unit in immunoassays and immunomagnetic cell sorting (IMCS) systems. (9)(10)(11) Broadly speaking, these devices are categorized as either passive or active, depending on the mixing strategy that they employ. Specific channel geometry configurations are used in passive micromixers to increase the surface contact area between the mixing species.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method of Constructing an Electroosmotic Micromixer with Double-Side Electrodes for Biotechnological Applications

2015

Sensors and Materials

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In this study, we provide a simple, low-cost, and novel method of constructing microelectrodes on both sides of a fluid microchannel to be utilized as an electroosmotic micromixer. The electrodes mounted on both sides of the microchannel provide unique characteristics for biological studies, electrokinetics, dielectrophoresis, and so forth. The fabrication process includes optical lithography, layer deposition to create electrodes, accurate alignment, and bonding of two wafers. Compared with other methods used for fabricating electrodes on both sides of microchannels, the method proposed in this study does not require very difficult and complex experimental conditions, cumbersome processes and expensive equipment. The distribution of the electric field is numerically analyzed for two electrode arrangements. The numerical analysis shows that the efficiency of the electrodes mounted on both sides of the microchannel is higher than that of the electrodes mounted on one side. The proposed technique is a good alternative to the one-side electrodes and other double-side electrode configurations for electroosmotic micromixers. The process simplicity provides an easy way of constructing microelectrodes on both sides of the microchannel for mixing fluids.

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Section: Introductionmentioning

confidence: 99%

A Novel Method of Constructing an Electroosmotic Micromixer with Double-Side Electrodes for Biotechnological Applications

2015

Sensors and Materials

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“…As a gradient generator, micromixers have a number of novel biological applications in assays based on concentration gradient such as the studies of cancer metastasis and stem cell growth and differentiation [6][7]. They are also defined as a labeling unit in Immunoassays and immunomagnetic cell sorting systems (IMCS) [8][9].…”

Section: Introductionmentioning

confidence: 99%

Numerical study of a novel microfluidic electroosmotic mixer with high and fast throughput

Talebzadeh

Veladi

2014

2014 Second RSI/ISM International Conference on Robotics and Mechatronics (ICRoM)

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In this paper the design and numerical simulation of a novel microfluidic electroosmotic micromixer with high throughput and fast mixing time is presented. Due to constant outlet flow rate, some local narrow channel geometries through the mixing channel are created to achieve fast response and shorter mixing time. This micromixer is equipped with two sets of electrode arrays separated by a narrow channel within the mixing area. The microelectrodes are embedded on the side surfaces of the microchannel. The electrode array consists of two sets of four 20 µm and 40 µm microelectrodes. Numerical analysis of electric potential and mean inlet flow velocity effects on mixing efficiency of the micromixer is carried out by means of two sets of simulations in steady state and transient state. First, the electric potential is kept constant at 3 V while the mean inlet flow velocity is varied within 0.1-0.3 mm/s with 0.05 mm/s steps. The highest achieved mixing efficiency is 95.5% at 0.1 mm/s. Next, the mean inlet flow velocity is kept at 0.1 mm/s whilst the electric potential is swept within 0-3.5 V. The best achieved mixing efficiency is 95.7% at 3.5 V.

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“…Recently, an increasing number of researchers have integrated micromixers into their analysis systems. The synthesis of active ingredients for the pharmaceutical industry [ 1 ] and the studies of immuno-magnetic cell sorting [ 2 ] are two examples. Many researches on the miniaturization of chemical analysis systems have considered the reactions of solutions in a microsystem.…”

Section: Introductionmentioning

confidence: 99%

Optimal Designs of Staggered Dean Vortex Micromixers

Chen

Shie

2011

IJMS

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A novel parallel laminar micromixer with a two-dimensional staggered Dean Vortex micromixer is optimized and fabricated in our study. Dean vortices induced by centrifugal forces in curved rectangular channels cause fluids to produce secondary flows. The split-and-recombination (SAR) structures of the flow channels and the impinging effects result in the reduction of the diffusion distance of two fluids. Three different designs of a curved channel micromixer are introduced to evaluate the mixing performance of the designed micromixer. Mixing performances are demonstrated by means of a pH indicator using an optical microscope and fluorescent particles via a confocal microscope at different flow rates corresponding to Reynolds numbers (Re) ranging from 0.5 to 50. The comparison between the experimental data and numerical results shows a very reasonable agreement. At a Re of 50, the mixing length at the sixth segment, corresponding to the downstream distance of 21.0 mm, can be achieved in a distance 4 times shorter than when the Re equals 1. An optimization of this micromixer is performed with two geometric parameters. These are the angle between the lines from the center to two intersections of two consecutive curved channels, θ, and the angle between two lines of the centers of three consecutive curved channels, ϕ. It can be found that the maximal mixing index is related to the maximal value of the sum of θ and ϕ, which is equal to 139.82°.

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Performance Assessment and Improvement of a Spilt-and-Recombine Micromixer for Immunomagnetic Cell Sorting

Cited by 6 publications

References 14 publications

A Novel Method of Constructing an Electroosmotic Micromixer with Double-Side Electrodes for Biotechnological Applications

A Novel Method of Constructing an Electroosmotic Micromixer with Double-Side Electrodes for Biotechnological Applications

Numerical study of a novel microfluidic electroosmotic mixer with high and fast throughput

Optimal Designs of Staggered Dean Vortex Micromixers

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