Performance Assessment of The Polymerase Chain Reaction-restriction Fragment Length Polymorphism Method for Rapid Detection of Susceptibility to Ethambutol and Molecular Prediction of XDR Tuberculosis in Clinical Isolates of Mycobacterium Tuberculosis

Nazari, Razieh; Zolfaghari,; Taherahmadi, M; Sadrnia, Maryam; Titov, L. P.; Ahmadi, Azam; Shojapoor, Mana

doi:10.7727/wimj.2014.022

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“…Many of these compounds (particularly ofloxacin) can penetrate the cells and is active against intracellular MTB [ 7 , 8 ]. Ofloxacin is highly active against MTB, and is an important drug in tuberculosis treatment [ 3 , 7 , 9 ].…”

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confidence: 99%

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Oﬂoxacin

et al. 2017

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The aim of the study was to investigate behavior of resistant Mycobacterium tuberculosis (MTB) isolates under a high dose of ofloxacin and its morphological changes. 19 extensively drug resistant (XDR) clinical isolates of MTB were grown on Löwenstein–Jensen medium containing progressively increasing concentrations of ofloxacin (2, 4, 8, 16, 32 mg/L). Ultra-structure analyses of resistant isolates grown on ofloxacin were conducted by transmission electron microscopy (TEM). Fixation was carried out by 4% glutaraldehyde in 0.1 M sodium cacodylate buffer on 300 mesh carbon formvar copper grid. The samples were negatively stained with uranium acetate suspension. All 19 XDR MTB isolates were grown and formed colonies successfully on 2, 4, 8 mg/L, seven isolates on 16 mg/L, and four isolates on 32 mg/L ofloxacin. Morphological changes and unusual forms were detected in 8, 16 and 32 mg/L ofloxacin at 43%, 76.5% and 81% of cells, respectively. Swollen form (protoplast like), ghost-like cell, degraded forms, and in a few cases, detached cytoplasm from cell wall were clearly detected in high drug concentrations in comparison to control. Changes in morphology were increased with increasing ofloxacin concentrations (p < 0.05). Some XDR isolates could be successfully grown on high doses of ofloxacin (32 mg/L), but with changes in morphology. It was concluded that several magnitudes of the drug doses could not prevent growth of drug resistant forms.

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Section: Introductionmentioning

confidence: 99%

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Oﬂoxacin

et al. 2017

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Performance Assessment of The Polymerase Chain Reaction-restriction Fragment Length Polymorphism Method for Rapid Detection of Susceptibility to Ethambutol and Molecular Prediction of XDR Tuberculosis in Clinical Isolates of Mycobacterium Tuberculosis

Abstract: Introduction: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was employed for rapid detection of ethambutol (EMB) resistant clinical isolates of

Cited by 1 publication

References 18 publications

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Oﬂoxacin

Transmission Electron Microscopy of XDR Mycobacterium tuberculosis Isolates Grown on High Dose of Oﬂoxacin

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