Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System

Moothanchery, Mohesh; Pramanik, Manojit

doi:10.3390/s17020357

Cited by 60 publications

(57 citation statements)

References 31 publications

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“…In previously reported studies, 12,27 the time taken to complete OR-scan (4.5 mm × 5 mm) area was 50 min (step size of 2 μm in the Y direction and 3 μm in the X direction). AR-PAM will take 8 min to complete same area (step size of 15 μm in the Y direction and 15 μm in the X direction).…”

Section: Resultsmentioning

confidence: 99%

High-speed simultaneous multiscale photoacoustic microscopy

Moothanchery

Kim

et al. 2019

J. Biomed. Opt.

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Photoacoustic microscopy (PAM) is a fast-growing biomedical imaging technique that provides highresolution in vivo imaging beyond the optical diffusion limit. Depending on the scalable lateral resolution and achievable penetration depth, PAM can be classified into optical resolution PAM (OR-PAM) and acoustic resolution PAM (AR-PAM). The use of a microelectromechanical systems (MEMS) scanner has improved OR-PAM imaging speed significantly and is highly beneficial in the development of miniaturized handheld devices. The shallow penetration depth of OR-PAM limits the use of such devices for a wide range of clinical applications. We report the use of a high-speed MEMS scanner for both OR-PAM and AR-PAM. A high-speed, wide-area scanning integrated OR-AR-PAM system combining MEMS scanner and raster mechanical movement was developed. A lateral resolution of 5 μm and penetration depth ∼0.9-mm in vivo was achieved using OR-PAM at 586 nm, whereas a lateral resolution of 84 μm and penetration depth of ∼2-mm in vivo was achieved using AR-PAM at 532 nm. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Resultsmentioning

confidence: 99%

High-speed simultaneous multiscale photoacoustic microscopy

Moothanchery

Kim

et al. 2019

J. Biomed. Opt.

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“…The imaging system used for data acquisition has been previously described in References . The schematic of the AR‐PAM setup used for imaging is shown in Figure .…”

Section: Methodsmentioning

confidence: 99%

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

Mozaffarzadeh

Varnosfaderani

Sharma

et al. 2019

Journal of Biophotonics

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In acoustic-resolution photoacoustic microscopy (AR-PAM) systems, the lateral resolution in the focal zone of the ultrasound (US) transducer is determined by the numerical aperture (NA) of the transducer. To have a high lateral resolution, a large NA is used. However, the larger the NA, the smaller the depth of focus [DOF]. As a result, the lateral resolution is deteriorated at depths out of the focal region. The synthetic aperture focusing technique (SAFT) along with a beamformer can be used to improve the resolution outside the focal region. In this work, for image formation in AR-PAM, we propose the double-stage delay-multiply-and-sum (DS_DMAS) algorithm to be combined with SAFT. The proposed method is evaluated experimentally using hair targets and in vivo vasculature imaging. It is shown that DS_DMAS provides a higher resolution and contrast compared to other methods. For the B-mode images obtained using the hair phantom, the proposed method reduces the average noise level for all the depths by about 134%, 57% and 23%, compared to the original low-resolution, SAFT+DAS and SAFT+DMAS methods, respectively. All the results indicate that the proposed method can be an appropriate algorithm for image formation in AR-PAM systems. K E Y W O R D S acoustic-resolution photoacoustic microscopy, contrast enhancement, synthetic aperture focusing technique, vasculature imaging, virtual source Moein Mozaffarzadeh, Mehdi H. H. Varnosfaderan and Arunima Sharma contributed equally to this study.

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“…For example, in optical resolution photoacoustic microscopy (OR-PAM), typical imaging depth up to 1 to 2 mm is obtained with lateral resolution of 0.25 to 5 micron, 180,181 and in acoustic resolution microscopy (AR-PAM), 3 to 8 mm imaging depth is obtained with lateral resolution of 40 to 50 micron. 182,183 Most of the PAM systems reported so far have used visible or NIR-I window due to the strong hemoglobin absorption. Recently, OR-PAM was reported for the first time in the second NIR window.…”

Section: High-resolution Photoacoustic Imaging In Near-infrared-ii Wimentioning

confidence: 99%

Photoacoustic imaging in the second near-infrared window: a review

2019

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Photoacoustic (PA) imaging is an emerging medical imaging modality that combines optical excitation and ultrasound detection. Because ultrasound scatters much less than light in biological tissues, PA generates high-resolution images at centimeters depth. In recent years, wavelengths in the second near-infrared (NIR-II) window (1000 to 1700 nm) have been increasingly explored due to its potential for preclinical and clinical applications. In contrast to the conventional PA imaging in the visible (400 to 700 nm) and the first NIR-I (700 to 1000 nm) window, PA imaging in the NIR-II window offers numerous advantages, including high spatial resolution, deeper penetration depth, reduced optical absorption, and tissue scattering. Moreover, the second window allows a fivefold higher light excitation energy density compared to the visible window for enhancing the imaging depth significantly. We highlight the importance of the second window for PA imaging and discuss the various NIR-II PA imaging systems and contrast agents with strong absorption in the NIR-II spectral region. Numerous applications of NIR-II PA imaging, including whole-body animal imaging and human imaging, are also discussed.

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Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System

Cited by 60 publications

References 31 publications

High-speed simultaneous multiscale photoacoustic microscopy

High-speed simultaneous multiscale photoacoustic microscopy

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

Photoacoustic imaging in the second near-infrared window: a review

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