In two differential conditioning experiments, groups of 10 rats each differed with respect to average reward and schedule of reward received in S+. Nonreward (N) occurred on all Strials. In both experiments, extinction of responding to S-(resistance to discrimination) was extensively regulated by reward sequence and was largely independent of average reward. In Experiment I, resistance to discrimination was a function of transitions from N to rewarded (R) trials (N-R transitions). In Experiment 2, resistance to discrimination was increased by large reward on the R trial of N-R transitions and decreased by large reward on the R trial of R-N transitions. These schedule effects on resistance to discrimination parallel the effects of comparable schedules on resistance to extinction following partial reinforcement. The results are discussed in terms of sequential theory, reinforcement level theory, and their implications for various schedule manipulations that have previously shown S-behavior to be inversely related to average reward in S+.Two factors have been shown to be particularly important in regulating behavior to the negative (S-) stimulus during differential conditioning, trial sequence (Capaldi, Berg, & Morris, 1975;Haggbloom, 1978Haggbloom, , 1979Haggbloom, , 1980McHose & Blackwell, 1975) and the difference between the average incentive associated with the two discriminanda (McHose, 1970).The incentive averaging view is illustrated by an experiment reported by McHose, Maxwell, and McHewitt (1971, Experiment 4). In that experiment, all groups received nonreward (N) in S-; one group received eight and another two 45-mg food pellets on all S+ trials; and a third group, crucial to the incentive averaging view, received a 50% partial reinforcement (PRF) schedule in S+, obtaining 16 pellets on rewarded (R) trials. Where the numerals indicate S+ and S-reward, these groups can be identified, respectively, as Groups 8-0, 2-0, and 16/0-0.Using formulas suggested by McHose (1970) for computing average incentive, McHose et al. (1971) showed that the average incentive disparity between S+ and S-was equal in Groups 16/0-0 and 2-0 and less than that in Group 8-0. Consistent with the incentive averaging view, Groups 16/0-0 and 2-0 performed alike in S-and ran faster than Group 8-0.