Performance of a rosette assay between lymphocytes and sheep erythrocytes at elevated temperatures to study patients with cancer and other diseases

West, William H.; Sienknecht, Charles W.; Townes, Alexander S.; Herberman, Ronald B.

doi:10.1016/0090-1229(76)90149-5

Cited by 68 publications

(23 citation statements)

References 15 publications

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“…This technique used by other authors did not prove to be recommendable for identi fication of all T cells since even subtle modifi cations resulted in significant dispersion of the T cell values (Bach, 1973;Froland and Natvig, 1973). On the other hand, in most reports, no care was taken to discriminate between the age of the individuals examined West et al, 1976). Thus, the fluctuations in the proportions of T lympho cytes determined by the method of 1 -2 h incubation might be due either to technical problems or to the age variations in randomly chosen individuals.…”

mentioning

confidence: 99%

Proportion of Early E Rosettes Formed by Peripheral Blood Lymphocytes from Young and Aged Subjects

Myśliwska¹,

Witkowski²,

Myśliwski³

1981

Gerontology

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The absolute number and percentage of early E-rosette-forming cells were determined in blood of young and aged subjects. The assay was carried out at low temperature (+ 4°C) for 30 min in phosphate-buffed saline without any supplementation. The results indicate that both the absolute number and the percentage of early E rosettes detected are age-dependent, showing a tendency of reaching higher values in both aged females and males. There were also sex-related differences between young males and young females, but the values for aged males and females were approximately the same. The significance of the above-described early E rosette values is discussed and compared with early E rosettes detected by other methods.

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mentioning

confidence: 99%

Proportion of Early E Rosettes Formed by Peripheral Blood Lymphocytes from Young and Aged Subjects

Myśliwska¹,

Witkowski²,

Myśliwski³

1981

Gerontology

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“…Several normal donors had one abnormal rosette determination between normal values. Usually these were found to have one of the conditions previously shown to depress E-RFC levels [4] eg, virus infection. In the few remaining donors with occasional depression, no cause was found.…”

Section: Resultsmentioning

confidence: 97%

“High‐affinity” t‐cell rosettes: The effects of clinical manipulations and potential prognostic significance

Weese¹,

West²,

Herberman³

et al. 1980

Journal of Surgical Oncology

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Incubation of blood mononuclear cells with sheep erythrocytes at 29OC identifies "high-affinity'' rosette-forming T-cells (RFC). The proportion o f highaffinity RFC was more frequently depressed in patients with cancer than the total number of RFC identified under optimal rosetting conditions. Our purpose was to determine the effect of chemotherapy and/or immunotherapy on the level of RFC and t o determine whether trends in serial values had prognostic significance.Two hundred thirty-seven of 270 normal individuals tested had 29OC RFC levels greater than or equal t o 47%, while 21 of 41 untreated cancer patients had higher levels. Cancer patients with depressed 29OC RFC usually normalized within four weeks of operation. Serial studies of 29°C RFC were performed on 32 stage I and I1 lung cancer patients. Eight patients have developed recurrent tumor. We noted a pattern of falling rosette values (two or more consecutive values within normal limits followed by two consecutive depressed values) in five of these patients an average of five months before clinical relapse. Fifteen patients who have remained disease free either maintained normal levels of rosettes or increased into the normal range. Four of 13 (3 1%) stage I and four of nine (44%) stage I1 breast cancer patients had depressed rosette levels preoperatively. In seven stage I1 patients with no further therapy after mastectomy, only t w o of 27 serial tests showed low RFC. Twenty-six stage I1 patients receiving L-phenylalanine mustard (L-PAM) therapy after mastectomy were depressed in 18 of 90From the

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“…Thymus-dependent lymphocytes were assayed when possible prior to therapy and at monthly intervals by determination of spontaneous rosette formation with sheep red blood cells (SRBC) at 4 ° C and at 29 ° C as previously described (West, 1978;West et al, 1976). Venous blood specimens were drawn prior to the administration of the next course of chemotherapy or after completion of chemotherapy.…”

Section: Rosettesmentioning

confidence: 99%

Immunologic monitoring and immunotherapy in Ewing's sarcoma

Richardson

Oldham

Pomeroy

et al. 1978

Cancer Immunol Immunother

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Performance of a rosette assay between lymphocytes and sheep erythrocytes at elevated temperatures to study patients with cancer and other diseases

Cited by 68 publications

References 15 publications

Proportion of Early E Rosettes Formed by Peripheral Blood Lymphocytes from Young and Aged Subjects

Proportion of Early E Rosettes Formed by Peripheral Blood Lymphocytes from Young and Aged Subjects

“High‐affinity” t‐cell rosettes: The effects of clinical manipulations and potential prognostic significance

Immunologic monitoring and immunotherapy in Ewing's sarcoma

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