Performance of Aerobic Denitrification by the Strain Pseudomonas balearica RAD-17 in the Presence of Antibiotics

Ruan, Yunjie; Cai, Lei; Lu, Haijun; Zhang, Meng; Xu, Xiangyang; Li, Wenbing

doi:10.3390/microorganisms9081584

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…Sulfamethoxazole (>2 μg/L) damages denitrifying cells, reduce bacterial electron-transport activity, and inhibit N 2 O reduction, leading to a reduction in aerobic denitrification nitrogen-removal efficiency in P. stutzeri PCN-1 (Gui et al, 2017). However, the nitrogen-removal efficiency of P. balearica RAD-17 through the aerobic denitrification pathway is insensitive to low and high concentrations of antibiotics such as ciprofloxacin and hygromycin (Ruan et al, 2021).…”

Section: Effect Of Antibiotics On Hnadsmentioning

confidence: 99%

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Song²,

Wang³

et al. 2022

Front. Environ. Sci.

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With the increasing use of animal and plant proteins, pollution due to nitrogen sources is attracting increasing attention. In particular, the amount of nitrogen-containing sewage discharged into the environment has increased significantly, causing eutrophication of water bodies and environmental degradation of water quality. Traditionally, nitrifying bacteria perform ammonia nitrification under aerobic conditions, while denitrifying bacteria perform nitrate/nitrite denitrification under anaerobic conditions. However, heterotrophic nitrifying and aerobic denitrifying microorganisms (HNADs) perform ammonia nitrification and nitrate/nitrite denitrification under the same aerobic conditions using an organic carbon source, which is a much simpler and more efficient process. In this review, the distribution and evolutionary relationships of novel HNADs strains are presented, and the influencing factors, metabolic pathways, key enzymes, and practical applications of HNADs are reviewed.

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Section: Effect Of Antibiotics On Hnadsmentioning

confidence: 99%

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Song²,

Wang³

et al. 2022

Front. Environ. Sci.

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“…Moreover, in order to make further efforts to clarify the mechanism of aerobic denitrification and make the aerobic denitrification process more economical and efficient, the kinetic affinity index [ 24 , 25 ] and the regulatory mechanisms of intracellular electron transfer [ 26 ] were characterized. Different antibiotics inhibition [ 8 , 27 ] and types of biofilm carriers [ 28 , 29 ] were also evaluated. However, the detailed mechanism and effective regulation have not been fully mastered, and the influence of ion species and concentrations is one of them.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ion Combinations and Their Concentrations on Denitrification Performance and Gene Expressions of an Aerobic Strain Marinobacter Hydrocarbonoclasticus RAD-2

Cai

et al. 2023

Microorganisms

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Salinity is one of the most important factors affecting the nitrogen-removal efficiency of denitrifying bacteria. A series of different ion combinations and salinity gradients were carried out to clarify the effects of ion types and concentrations on nitrogen removal by halophilic aerobic denitrifying bacteria RAD-2. Nitrate concentrations, nitrite concentrations, TAN concentrations, and OD600 were monitored to investigate their effects on denitrification in each group. The results showed that Na+, K+, and Cl- accelerated the denitrification process and improved nitrogen-removal efficiency at moderate additions, while Ca2+ and Mg2+ showed no significant effect. Na+ was effective alone, while K+ or Cl- needed to be combined with at least one of Na+, K+, or Cl- to achieve similar efficiency. The batch tests of salinity confirmed that the addition of a moderate concentration of NaCl/Na2SO4 could effectively improve nitrogen-removal efficiency, while excessive salinity might hinder denitrification metabolism. In the salinity range of 5~40‰, a 5‰ dosage might be the most economical method for strain RAD-2. Real-time PCR experiments on 17 key nitrogen metabolism-related genes revealed that chloride was widely involved in the nitrogen and carbon metabolism of microorganisms by altering cell osmotic pressure and opening ion channel proteins, thereby affecting the efficiency of denitrification. The results of this study may contribute to a better understanding of the different roles of various ions in aerobic denitrification and highlight the importance of salinity control in highly salted wastewater treatment.

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“…al. 2017),Pseudomonas(Ruan, et al 2021) and Thiobacillus(Sublette 1987) were more abundant in Group Y. Thiobacillus can not only oxidise hydrogen sulphide aerobically, but also participates in denitri cation(Sublette 1987). Additionally, more Vibrionaceae were detected in Group Y (5.99%) in November; members of this family are pathogenic and cause severe economic losses in aquaculture(Banchi, et al.…”

mentioning

confidence: 99%

Cultivation of Ulva lactuca changes bacterial community structure and abundances of nitrogen cycling functional genes in an IMTA system

Kong

Chen

Zhao

et al. 2023

Preprint

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To study nitrogen removal by Ulva lactuca and its effects on the structure of the bacterial community and abundance of nitrogen cycle functional genes in the ‘sea cucumber–shrimp–crab–fish’ integrated multi-trophic aquaculture (IMTA) system, we analysed bacterial community composition by Illumina MiSeq high-throughput sequencing and nitrogen cycling functional gene levels by real-time quantitative PCR in water and sediment with (Y) and without the presence of U. lactuca (N). Diversity and abundance indices, including Sobs, Shannon, Ace, and Chao1, were higher in Group Y water and sediment. In the water, the predominant phyla were Proteobacteria in Group Y and Cyanobacteria in Group N in September. Flavobacteriaceae and Rhodobacteraceae were the dominant families in Group Y and Group N in October and November, respectively. In the sediment, Bacillaceae was the dominant family in Group Y throughout the experimental period, whereas Desulfocapsaceae was the dominant family in Group N in October and November. Regarding nitrogen cycle functional genes, millions of Amx, nosZ, nxrB, and nrfA genes were detected in both water and sediment. The abundances of nifH, amoA, nxrB, norB, and nrfA genes were higher in Group Y than those in Group N, which can improve ammonia removal without nitrogen loss. In this study, a potential novel approach was proposed to enhance the development of sustainable aquaculture, promote the nitrogen cycle, and remediate and optimise the water environment by cultivating bacteria and algae, according to the effects of U. lactuca on water quality, bacterial communities, and abundance of functional nitrogen cycling genes in IMTA systems.

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Performance of Aerobic Denitrification by the Strain Pseudomonas balearica RAD-17 in the Presence of Antibiotics

Cited by 6 publications

References 32 publications

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Advances in Research Into and Applications of Heterotrophic Nitrifying and Aerobic Denitrifying Microorganisms

Effects of Ion Combinations and Their Concentrations on Denitrification Performance and Gene Expressions of an Aerobic Strain Marinobacter Hydrocarbonoclasticus RAD-2

Cultivation of Ulva lactuca changes bacterial community structure and abundances of nitrogen cycling functional genes in an IMTA system

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