Performance of airlift bioreactors in the cultivation of some antibiotic producing microorganisms

Roman, R. V.

doi:10.1002/abio.370180304

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…Gravilescu and Roman (1998) showed that airlift reactors consistently had higher energy efficiencies of between 30% and 40% in a number of antibiotic fermentations, when compared with stirred tank reactors. This reflects a general trend of airlift reactors being more energy efficient than stirred tanks (Chisti, 1989).…”

Section: Power Consumptionmentioning

confidence: 99%

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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: There is a lack of research into bioreactor engineering and fermentation protocol design in the field of marine bacterial antibiotic production. Most production strategies are carried out at the shake-flask level and lack a mechanistic understanding of the antibiotic production process, offering poor prospects for successful scale-up. This review shows that data need to be collated on media and physical optima differences between the trophophase and idiophase, along with investigations into the control mechanisms for biosynthesis, to allow implementation of novel fermentation protocols. Immobilization may play a part in bioprocess intensification of marine bacterial antibiotic production, through again this area is understudied. Similarly, mass transfer and shear stress data of fermentations are needed to provide the bioreactor design requirements to intensify antibiotic biosynthesis, with process scale-up in mind. The application of bioprocess intensification methods to the production of antibiotics (and other metabolites) from marine microbes will become an important strategy for improving supply of natural products, in order to assess their suitability as chemotherapeutic drugs.

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Section: Power Consumptionmentioning

confidence: 99%

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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“…For aerobic cultures, air injection, besides being responsible for providing oxygen to the cells, decreases the density of the gasliquid dispersion inside the inner tube, thus promoting recirculation and mixing of the reaction medium in the bioreactor. This mixing behavior implies lower power consumption for this reactor type, as reported by KrackeHelmet et al [2] and Gavrilescu and Roman [3]. However, generally speaking, the absence of mechanical agitation by impellers in the airlift setup results in a lower oxygen transfer rate.…”

Section: Introductionmentioning

confidence: 64%

Oxygen transfer in a pressurized airlift bioreactor

Campani

Ribeiro

Horta

et al. 2015

Bioprocess Biosyst Eng

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Airlift bioreactors (ALBs) offer advantages over conventional systems, such as simplicity of construction, reduced risk of contamination, and efficient gas-liquid dispersion with low power consumption. ALBs are usually operated under atmospheric pressure. However, in bioprocesses with high oxygen demand, such as high cell density cultures, oxygen limitation may occur even when operating with high superficial gas velocity and air enriched with oxygen. One way of overcoming this drawback is to pressurize the reactor. In this configuration, it is important to assess the influence of bioreactor internal pressure on the gas hold-up, volumetric oxygen transfer coefficient (k(L)a), and volumetric oxygen transfer rate (OTR). Experiments were carried out in a concentric-tube airlift bioreactor with a 5 dm(3) working volume, equipped with a system for automatic monitoring and control of the pressure, temperature, and inlet gas flow rate. The results showed that, in disagreement with previous published results for bubble column and external loop airlift reactors, overpressure did not significantly affect k(L)a within the studied ranges of pressure (0.1-0.4 MPa) and superficial gas velocity in the riser (0.032-0.065 m s(-1)). Nevertheless, a positive effect on OTR was observed: it increased up to 5.4 times, surpassing by 2.3 times the oxygen transfer in a 4 dm(3) stirred tank reactor operated under standard cultivation conditions. These results contribute to the development of non-conventional reactors, especially pneumatic bioreactors operated using novel strategies for oxygen control.

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“…The airlift reactor was first patented for use as a bioreactor by Lefrancois in 1955 (Gavrilescu and Roman, 2004). Due to the fluid dynamic characteristics, it continues to gain popularity for its success in the production of microorganisms (Siegel et al, 1988;Kim et al, 1997).…”

Section: Types Of Bioreactorsmentioning

confidence: 99%

Mixing characteristics of draft tube airlift bioreactor using the electrical resistance tomography

Yakubu-Gumery¹

2021

Preprint

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In this work, mixing characteristics in terms of mixing time, hydrodynamics (liquid circulation velocity and gas hold up) and shear rate were performed in the downcomer of a draft tube airlift bioreactor with different geometries (i.e., Ad/Ar between 0.38 – 2.31 and bottom clearances between 0.003-0.00 m). Newtonian (water and 34.5% coalescing sugar solution) and on Newtonian (0.2% and 0.5% xanthan gum solutions) with different viscosities were used as the liquid phase. Compressed air was used as the gas phase which was introduced through cross and circular shaped sparger configurations at superficial velocities Ugr = 0.00165-0.00807 m/s. The combined effects of geometric parameters (Ad/Ar, bottom clearances), sparger configuration, and liquid viscosity on mixing characteristics have been presented. Results showed that the increase in superficial gas velocity (Ugr) corresponds to an increase in energy generated, and thus decreases in mixing time. However, the increase in Ugr corresponds to the increase in liquid circulation velocity, gas holdup and shear rate values. Moreover, bottom clearances and draft tube diameters show effects on flow resistance frictional losses which affect results of mixing parameters investigated. The influence of sparger configurations on mixing time and liquid circulation velocity is significant due to their effect on gas distribution. Mixing time decreased to about 40% in air-water media using the cross shaped sparger. Results obtained with cross shaped sparger showed even and uniform distribution of gas, which provided better mixing as compared to the circular shaped sparger configuration. However, the sparger configuration effect on shear rate is not as significant (about 20% reduction in shear rate values using the cross shaped sparger). The effect of fluid viscosity had a significant influence on both mixing times and circulation velocity, especially in the coalescing media of sugar and xanthan gum solutions. Results from this work will help to develop a clear pattern for operation and mixing that can help improving [sic] several industrial processes, especially the ones related to emerging fields of technology such as the biotechnology industry.

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Performance of airlift bioreactors in the cultivation of some antibiotic producing microorganisms

Cited by 11 publications

References 30 publications

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Oxygen transfer in a pressurized airlift bioreactor

Mixing characteristics of draft tube airlift bioreactor using the electrical resistance tomography

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