Performance of Paracoccus pantotrophus MA3 in heterotrophic nitrification–anaerobic denitrification using formic acid as a carbon source

Huang, Qun; Alengebawy, Ahmed; Zhu, Xiangyu; Amin, Farrukh Raza; Chen, Limei; Chen, Wuxi; Guo, Jiahao; Ai, Ping; Li, Demao

doi:10.1007/s00449-022-02771-3

Cited by 7 publications

(4 citation statements)

References 50 publications

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“…and Acinetobacter sp. YS2 have been commonly used either individually or in combinations for the nitrification and denitrification processes 75‐78 . A study using Acinetobacter sp.…”

Section: Resultsmentioning

confidence: 99%

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Performance appraisal of microbial inoculants treating polluted surface water through bench‐scale investigation

Simon

Joshi

Hazra

2023

J of Chemical Tech & Biotech

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BACKGROUNDThis study investigates the performance of microbial inoculants by exploring pollutant removal efficiency, microbial viability and substrate removal kinetics for treating polluted surface water. The morphological, elemental composition and biochemical characterization of inoculants were analyzed using field emission scanning electron microscopy with energy dispersive X‐ray spectroscopy (FESEM‐EDX) and Fourier transform infrared (FTIR) techniques. The identification of bacterial strains in polluted feed water also was performed using 16S rRNA partial sequencing. Bench‐scale efficacy of selected microbial inoculants (MI) along with control for each study was performed at variable retention times ranging from 6 to 72 h, under optimum operating conditions.RESULTSThe maximum removal (86% and 90%) of the organic load (chemical and biological oxygen demand, COD and BOD) was achieved by inoculants containing Bacillus subtilis along with other microbial species after 72 h of treatment. The microbial inoculant MI‐2 containing Paracoccus pantotrophus and Pseudomonas Putida exhibited the highest TN removal (66%) after 72 h of contact time. Adenosine triphosphate (ATP) analysis revealed that the active biomass ratio is a more precise indicator of healthy biomass instead of the conventionally used parameter MLVSS (mix liquor volatile suspended solids) in a biological treatment system, which apparently provides a significant addition to the existing knowledge base for the operational wastewater treatment plants (WTPs).CONCLUSIONIn this study, microbial inoculants were found to be frugal and viable treatment tools for the remediation of polluted surface water. Exploiting such inoculants also can overcome the inefficiencies of existing sewage treatment plants by serving as potential tools for bio‐augmenting the indigenous microbial fingerprint of aeration tank/secondary treatment systems. © 2023 Society of Chemical Industry (SCI).

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“…and Acinetobacter sp. YS2 have been commonly used either individually or in combinations for the nitrification and denitrification processes 75‐78 . A study using Acinetobacter sp.…”

Section: Resultsmentioning

confidence: 99%

“…YS2 have been commonly used either individually or in combinations for the nitrification and denitrification processes. [75][76][77][78] A study using Acinetobacter sp. YS2 reported similar efficiency in TN removal after a 5-day period.…”

Section: Removal Of Colour and Suspended Solidsmentioning

confidence: 99%

Performance appraisal of microbial inoculants treating polluted surface water through bench‐scale investigation

Simon

Joshi

Hazra

2023

J of Chemical Tech & Biotech

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“…The influent water did not contain NO 3 − -N or NO 2 − -N during the whole experimental cycle; however, the NO 3 − -N concentration in the effluent water was high in the preexperimental period because the bacteria were in the growth and reproduction stage, and they underwent a denitrification reaction with colony stabilization which converted NO 3 − -N into N 2 and reduced the nitrogen concentration in the water [26]. The average NO 3 − -N concentrations in the effluent during stabilization in T1, T2, and T3 were 8.16 ± 0.44 mg/L, 3.46 ± 0.37 mg/L, and 1.76 ± 0.15 mg/L, respectively, and NO 3 − -N accumulation occurred in T1 because of the lack of a carbon source to fulfill the energy demand of microorganisms for denitrification (Figure 1d).…”

Section: Effect Of Pga Dosage On Pollutant-removal Effectmentioning

confidence: 99%

Denitrification Performance and Microbiological Mechanisms Using Polyglycolic Acid as a Carbon Source

Wang,

Li,

Yang

et al. 2024

Water

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When treating municipal wastewater, nitrogen removal is often limited due to low C/N, which needs to be compensated for by additional carbon source injections. This study investigated the feasibility of using industrial-waste polyglycolic acid (PGA) as a carbon source for denitrification in an SBR to obtain an economical carbon source. The results revealed that an optimal denitrification performance in a methanol-fed activated sludge system was achieved with a PGA dosage of 1.2 mL/L, a pH of 7–8, and a dissolved-oxygen (DO) concentration of 3 ± 0.5 mg/L. Under these conditions, all quality parameters for effluent water met the required criteria [COD < 50 mg/L; TN < 15 mg/L; NH4+-N < 5(8) mg/L]. PGA enhanced the variety and richness of microbial communities, thereby markedly increasing the relative abundance of major phyla such as Proteobacteria and Bacteroidota and major genera such as Paracoccus and Dechloromonas. Furthermore, PGA upregulated the expression of nitrogen-metabolism-related genera, including amo, hao, nar, and nor, which improved the denitrification performance of the system. This study provides a reference for applying PGA as a carbon source for low-C/N-wastewater treatment and solid-waste utilization.

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“…Currently, the removal efficiency of nitrogen for most the reported HNAD bacteria is negatively impacted by the low shaking speed, and too low shaking speed can inhibit the nitrogen removal efficiency from bacteria (He et al, 2018;Chen et al, 2019; Zhang Huang et al, 2022). Interestingly, the different shaking speed conditions (0-200 rpm) had no effect on the nitrogen removal efficiencies of strain H7.…”

Section: Shaking Speedmentioning

confidence: 99%

Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7

Fan

Nie²,

Chen³

et al. 2023

Front. Microbiol.

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IntroductionNitrogen and arsenic contaminants often coexist in groundwater, and microbes show the potential for simultaneous removal of nitrogen and arsenic. Here, we reported that Hydrogenophaga sp. H7 was heterotrophic nitrification and aerobic denitrification (HNAD) and arsenite [As(III)] oxidation bacterium.MethodsThe appearance of nitrogen removal and As(III) oxidation of Hydrogenophaga sp. H7 in liquid culture medium was studied. The effect of carbon source, C/N ratio, temperature, pH values, and shaking speeds were analyzed. The impact of strains H7 treatment with FeCl3 on nitrogen and As(III) in wastewater was assessed. The key pathways that participate in simultaneous nitrogen removal and As(III) oxidation was analyzed by genome and proteomic analysis.Results and discussionStrain H7 presented efficient capacities for simultaneous NH4+-N, NO3−-N, or NO2−-N removal with As(III) oxidation during aerobic cultivation. Strikingly, the bacterial ability to remove nitrogen and oxidize As(III) has remained high across a wide range of pH values, and shaking speeds, exceeding that of the most commonly reported HNAD bacteria. Additionally, the previous HNAD strains exhibited a high denitrification efficiency, but a suboptimal concentration of nitrogen remained in the wastewater. Here, strain H7 combined with FeCl3 efficiently removed 96.14% of NH4+-N, 99.08% of NO3−-N, and 94.68% of total nitrogen (TN), and it oxidized 100% of As(III), even at a low nitrogen concentration (35 mg/L). The residues in the wastewater still met the V of Surface Water Environmental Quality Standard of China after five continuous wastewater treatment cycles. Furthermore, genome and proteomic analyses led us to propose that the shortcut nitrification-denitrification pathway and As(III) oxidase AioBA are the key pathways that participate in simultaneous nitrogen removal and As(III) oxidation.

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Performance of Paracoccus pantotrophus MA3 in heterotrophic nitrification–anaerobic denitrification using formic acid as a carbon source

Cited by 7 publications

References 50 publications

Performance appraisal of microbial inoculants treating polluted surface water through bench‐scale investigation

Performance appraisal of microbial inoculants treating polluted surface water through bench‐scale investigation

Denitrification Performance and Microbiological Mechanisms Using Polyglycolic Acid as a Carbon Source

Simultaneous removal of nitrogen and arsenite by heterotrophic nitrification and aerobic denitrification bacterium Hydrogenophaga sp. H7

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