Performance of RT-PCR in the detection of Streptococcus agalactiae in the anogenital tract of pregnant women

Feuerschuette, Otto May; Serratine, Ana Claudina Prudêncio; Bazzo, Maria Luiza; Martins, Toni Ricardo; Silveira, Sheila Koetker; Silva, Rosemeri Maurici da

doi:10.1007/s00404-012-2498-9

Cited by 16 publications

(17 citation statements)

References 26 publications

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“…The prevalence of GBS, preeclampsia, gestational diabetes, and infertility is similar to that reported globally [8–17]. Additionally, the rate of cesarean section in our participants was also similar to national trends [18].…”

Section: Commentsupporting

confidence: 87%

“Collection of a lifetime: A practical approach to developing a longitudinal collection of women's healthcare biological samples”

Santillan

Leslie

Hamilton

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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Objective The objective is to develop a biorepository of samples that represent all stages of a women’s life. Importantly, our goal is to collect longitudinal physical specimens as well as the associated short and long-term clinical information. Study Design The Women’s Health Tissue Repository was established to encompass four tissue banks: Well Women Tissue Bank, Reproductive Endocrinology & Infertility Tissue Bank, Maternal Fetal Tissue Bank, and the long-established Gynecologic Malignancies Tissue Bank. Based on their health status, women being seen in Women’s Health at the University of Iowa are recruited to contribute samples and grant access to their electronic medical record to the biorepository. Samples are coded, processed, and stored for use by investigators. Results The Maternal Fetal Tissue Bank was the first expansion of our department’s biobanking efforts. Approximately 75% of the women approached consent to participate in the Maternal Fetal Tissue Bank. Enrollment has steadily increased. Samples have been used for over 20 projects in the first 3 years and are critical to 7 funded grants and 3 patent applications. Conclusion Patient samples with corresponding clinical data are initially important to women’s health research. Our model demonstrates that many research projects by faculty, fellows, and residents have benefited from the existence of the Women’s Health Tissue Repository. While challenging to achieve, longitudinal sampling allows for the greatest opportunity to study normal and pathological changes throughout all phases of a women’s life, including pregnancy. This bank facilitates and accelerates the development of novel research, technologies, and possible therapeutic options in women’s health. The establishment of more longitudinal biorepositories based on our model would enhance women’s health research.

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Section: Commentsupporting

confidence: 87%

“Collection of a lifetime: A practical approach to developing a longitudinal collection of women's healthcare biological samples”

Santillan

Leslie

Hamilton

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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“…25 The detection of two different genetic targets using molecular amplification methods has been used as the gold standard criterion to define true positive results in other studies. 26,27 Therefore, these 27 cases might be actually representing a false negative culture result instead of false positive qPCR results. Our study was limited by a small population size in which nine subjects had to contribute two samples for the analysis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an in‐house real‐time polymerase chain reaction method to identify group B streptococcus colonization in pregnancy

Yeung

Cheung

Chau

et al. 2015

J of Obstet and Gynaecol

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“…The reason for this contrary may be related to detection of bacteria as a result of a higher sensitivity of PCR for detection of circulating nucleic acids, or non-proliferating, dead or degraded GBS (viable versus nonviable GBS); for instance, in antibiotic pretreated individuals. Other possibilities regarding this subject may be associated with remaining nucleic acid (microbial DNA aemia) mainly after successful antimicrobial therapy; in fact, the remaining nucleic acid may be detectable up to several days without evident clinical significance or positive culture result (12,27,28). Besides, presenting some none-GBS bacteria in the vaginogenital tract, such as enterococci or other streptococci species can inhibit the growth of GBS even when using a selective medium such as Lim broth.…”

Section: Discussionmentioning

confidence: 99%

Direct Identification of Streptococcus agalactiae in Vaginal Colonization in Pregnant Women Using Polymerase Chain Reaction

et al. 2014

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Background: Streptococcus agalactiae is a commensal organism, but it may cause infection in susceptible hosts including newborns as well as in pregnant or postpartum women. Applying rapid, accurate and sensitive methods for detecting group B Streptococcus (GBS) and receiving intrapartum antibiotic prophylaxis (IAP) at delivery have been demonstrated to increase the treatment possibility of carrier pregnant women and decrease the rates of GBS vertical transmission to infants. Objectives: The aim of this study was to compare the PCR assay results targeting 16S rRNA primers with the conventional culture method for direct detection of GBS in vaginal specimens of pregnant women at 35-37 weeks of gestation in Hamadan, Iran. Patients and Methods: A total of 203 vaginal specimens of pregnant women at 35-37 weeks of pregnancy from June 2013 through February 2014 were evaluated for detection of GBS, using culture method and polymerase chain reaction (PCR). Results: The prevalence of GBS in the 203 collected samples was 7.39% using culture method and 19.70% using PCR. Twenty-five specimens had positive PCR results and negative cultures; two specimens were positive in culture and negative by PCR.A total of 42 specimens (20.69%) were considered as true positive. The PCR results in comparison with culture (as the gold standard) revealed sensitivity of 88.24%, specificity of 87.44%, positive and negative predictive value of 35.71% and 98.95% respectively, and accuracy of 87.50%. Conclusions: Performing only culture method led to missed false negative carriers. Therefore, it is recommended that both the PCR assay and conventional culture method be routinely performed to detect GBS in pregnant women accurately. PCR diagnosis demonstrated a shorter turnaround time when compared with the time consuming culture method.

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Performance of RT-PCR in the detection of Streptococcus agalactiae in the anogenital tract of pregnant women

Abstract: Further studies using other DNA extraction techniques, targeting other GBS genes and using sample enhancement before RT-PCR should be conducted to determine whether the sensitivity and specificity recommended by the CDC may be reached using the same thermal cycler.

Cited by 16 publications

References 26 publications

“Collection of a lifetime: A practical approach to developing a longitudinal collection of women's healthcare biological samples”

“Collection of a lifetime: A practical approach to developing a longitudinal collection of women's healthcare biological samples”

Evaluation of an in‐house real‐time polymerase chain reaction method to identify group B streptococcus colonization in pregnancy

Direct Identification of Streptococcus agalactiae in Vaginal Colonization in Pregnant Women Using Polymerase Chain Reaction

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