Entrepreneurship 4.0 has created bridges, generated connections, gathering and adding productive, technological resources and physical efforts in the development of entrepreneurial activity [1]. In this sense, it is possible to infer that the adoption of Innovation and Information and Communication Technology (ICT) resources by the entrepreneur can, in general, improve the process ofEntrepreneurial Education (EE). This article aims to understand some of the factors that directly influence training for entrepreneurship 4.0 as a new vector of growth and development in Brazilian regions. Methodologically, the development of the study took place from a broad survey of the literature on the evolution towards training for entrepreneurship 4.0 and data from a study with the participation of the main author carried out in Brazil adopting the statistical approach of Exploratory Factor Analysis (EFA) and supported by SPSS® version 19 software. The literature analysis and the application of the statistical method contributed to the identification of three factors (Competitive Advantage, Strategy and Entrepreneurial Profile),41.7%, and, consequently, will serve as a guideline for EE training for entrepreneurship 4.0. Although conceived in a specific context, the instrument generated can be applied, with the necessary adjustments, in other national and international experiences.