Performing ICSI with commercial microinjection pipettes enhanced pregnancy rates

Abstract

Background/aim: Many technical factors can affect intracytoplasmic sperm injection (ICSI) outcomes. The role of the injection micropipette could be of vital importance in ICSI programs. The main goal was to compare ICSI pregnancy outcomes between commercial and home-made injection micropipettes in a large population with male factor infertility.Materials and methods: Five-hundred and eleven ICSI cycles with severe male factor were included in this retrospective study. ICSI cycles were divided into two groups: A (home-made micropipettes, n = 267) and B (commercial micropipettes, n = 244). Rates of fertilization, embryo formation, and chemical and clinical pregnancies were compared between the groups. The independent samples t-test, chi-square test, and Fisher's exact test were used, whenever appropriate, for statistical analysis.Results: A total of 3621 MII oocytes were retrieved, of which 2003 were fertilized. The rate of normal fertilization was significantly higher in group A (57.9%) compared to group B (52.5%). However, the rate of embryo formation showed an increase in group B compared to group A (90.4% and 85.9%, respectively, P = 0.002). In addition, the clinical pregnancy outcomes improved in group B. Conclusion:Our findings indicate that clinical pregnancy improves when commercial injection micropipettes are used in ICSI programs.

"New Perspectives on Technical Aspects of Micromanipulation (ICSI) in Clinical Assisted Reproduction"

“…The lower incidence of degeneration and the higher normal fertilization rate and better embryo development and faster cleavage from ICSI using the smallest-diameter (3-5 µm) injection pipette was likely a result of minimized degree of the mechanical trauma exerted on the oolemma, minimized amount of ooplasm aspirated into the injection pipette prior to injection, and minimized the amount of PVP injected into the oocyte along with the spermatozoon [33]. At the time of oolemma breakage, reducing the volume of aspirated cytoplasm into the injection pipette can improve the ICSI success rate.…”

“…Khalili and teammate indicated that pregnancy improves when commercial injection micropipettes are used in ICSI program. They compared ICSI outcome using commercial versus home-made injection micropipettes in a large population with severe male infertility [33].…”

A Review of Nano/Micro/Milli Needles Fabrications for Biomedical Engineering

“…Puncture needle, solid millineedle, is an indispensable medical device in medical surgery [140], which have been widely used for tissue puncture, tissue extraction and so on [141]. For example, doctors can get an accurate and reliable diagnosis by puncturing the tissue with puncture needle to analyze the intrinsic differences in tissue cells [142].…”