Performing Quantitative Nanomechanical AFM Measurements on Live Cells

Pittenger, Bede; Slade, Andrea

doi:10.1017/s1551929513001077

Cited by 21 publications

(27 citation statements)

References 16 publications

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“…However, BPs have a higher deformation and energy dissipation than MPs (Figures 2B-C, and 2E-F). 21 The large particle deformation may be attributed to the high flexibility of BPs containing lipid bilayer shell, since previous study indicates that the selfassembled lipid bilayer structure could display fluidic and elastic properties. 24 In addition to particle flexibility, we also discuss the mechanical variation of MPs and BPs from an energetic aspect.…”

Section: Afm Characterization Of Flexibility Difference Between Mps Amentioning

confidence: 98%

“…To verify this hypothesis, the two kinds of nanoparticles are characterized by atomic force microscope (AFM)-based nanomechanical mapping. [21][22][23] Figures 2A and 2D are the typical topographies of MPs and BPs (more AFM images in Figure S5). Both MPs and BPs show the ball shape, the diameter of which is similar to Cryo-TEM observation.…”

Section: Afm Characterization Of Flexibility Difference Between Mps Amentioning

confidence: 99%

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Microfluidic Synthesis of Hybrid Nanoparticles with Controlled Lipid Layers: Understanding Flexibility-Regulated Cell–Nanoparticle Interaction

et al. 2015

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The functionalized lipid shell of hybrid nanoparticles plays an important role for improving their biocompatibility and in vivo stability. Yet few efforts have been made to critically examine the shell structure of nanoparticles and its effect on cell-particle interaction. Here we develop a microfluidic chip allowing for the synthesis of structurally well-defined lipid-polymer nanoparticles of the same sizes, but covered with either lipid-monolayer-shell (MPs, monolayer nanoparticles) or lipid-bilayer-shell (BPs, bilayer nanoparticles). Atomic force microscope and atomistic simulations reveal that MPs have a lower flexibility than BPs, resulting in a more efficient cellular uptake and thus anticancer effect than BPs do. This flexibility-regulated cell-particle interaction may have important implications for designing drug nanocarriers.

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Section: Afm Characterization Of Flexibility Difference Between Mps Amentioning

confidence: 98%

Section: Afm Characterization Of Flexibility Difference Between Mps Amentioning

confidence: 99%

Microfluidic Synthesis of Hybrid Nanoparticles with Controlled Lipid Layers: Understanding Flexibility-Regulated Cell–Nanoparticle Interaction

et al. 2015

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“…In contrast, Sr 2+ ions follow the step-pinning model, resulting in a highly irregular growth process 22 as visible in Figure 2D-E. Both ions are, to a certain extent, incorporated in the newly formed crystal 47 .…”

Section: Reference Experiments Differential Growth Of Sa Free Calcitementioning

confidence: 99%

“…[46][47][48] The maximum indentation force exerted by the tip on the sample during each tap is called 'peak force' and used as a feedback parameter to achieve a topographic image.…”

Section: Peakforce Quantitative Nanomechanical Property Mappingmentioning

confidence: 99%

Growth and Dissolution of Calcite in the Presence of Adsorbed Stearic Acid

Ricci¹,

Segura²,

Erickson³

et al. 2015

Langmuir

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The interaction of organic molecules with the surface of calcite plays a central role in many geochemical, petrochemical and industrial processes and in biomineralization. Adsorbed organics, typically fatty acids, can interfere with the evolution of calcite when immersed in aqueous solutions. Here we use atomic force microscopy in liquid to explore in real-time the evolution of the (101 ത 4) surface of calcite covered with various densities of stearic acid and exposed to different saline solutions. Our results show that the stearic acid molecules tend to act as 'pinning points' on the calcite's surface and slow down the crystal's restructuring kinetics. Depending on the amount of material adsorbed, the organic molecules can form monolayers or bilayer islands that become embedded into the growing crystal. The growth process can also displaces the organic molecules and actively concentrate them into stacked multilayers. Our results provide molecular-level insights into the interplay between the adsorbed fatty acid molecules and the evolving calcite crystal, highlighting mechanisms that could have important implications for several biochemical and geochemical processes and for the oil industry.

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“…One of widely applied approaches is to use sinusoidal modulation of the relative motion between the tip and the surface such as QNM (Bruker) mode. 52 Compared to conventional FS and FS-based force-volume mapping, the recording speed of this type of advanced FS has reached up to three magnitudes higher than that of the conventional FS with better positional accuracy and force control. [52][53][54] With advanced FS, the exploration of biological events in microsecond range and real molecular resolution became achievable.…”

Section: Advanced Fs-based Force-volume Mappingmentioning

confidence: 99%

Quantitative biomolecular imaging by dynamic nanomechanical mapping

et al. 2014

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The ability to 'see' down to nanoscale has always been one of the most challenging obstacles for researchers to address fundamental questions. For many years, researchers have been developing scanning probe microscopy techniques to improve imaging capability at nanoscale. Among them, atomic force microscopy (AFM) has received considerable attention, which allows probing topography of biological species at real space under physiological environment. Importantly, force measurements in AFM enable researchers to reveal not only the topography but also the relevant physical-chemical properties. AFM-based dynamic nanomechanical mapping (DNM) provides insights into the functions of biological systems by the interpretation of 'force', which are inaccessible by most of the other analytic techniques. This review is aiming to shed light on these recently developed AFM-based DNM techniques for biomolecular imaging, and discuss the relative applications in biological research from the nanomechanical point of view.

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Performing Quantitative Nanomechanical AFM Measurements on Live Cells

Cited by 21 publications

References 16 publications

Microfluidic Synthesis of Hybrid Nanoparticles with Controlled Lipid Layers: Understanding Flexibility-Regulated Cell–Nanoparticle Interaction

Microfluidic Synthesis of Hybrid Nanoparticles with Controlled Lipid Layers: Understanding Flexibility-Regulated Cell–Nanoparticle Interaction

Growth and Dissolution of Calcite in the Presence of Adsorbed Stearic Acid

Quantitative biomolecular imaging by dynamic nanomechanical mapping

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