Perfusion characteristics of parotid gland tumors evaluated by contrast-enhanced ultrasound

Klotz, Laura V.; Gürkov, Robert; Eichhorn, Martin; Siedek, Vanessa; Krause, Eike; Jauch, Karl‐Walter; Reiser, Maximilian; Clevert, D.‐A.

doi:10.1016/j.ejrad.2013.08.033

Cited by 21 publications

(42 citation statements)

References 19 publications

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“…Mainly, malignant neoplasms of the gland are slow growing tumors and are treatable when diagnosed in the early stage. In recent years, a variety of new imaging modalities have been developed in the diagnostics of salivary gland neoplasms [7,9,10]. CE-US represents a new promising ultrasound technique for the analysis of tumor microcirculation.…”

Section: Discussionmentioning

confidence: 99%

“…High resolution images, the lack of radiation exposure and the widespread availability characterises ultrasound as the diagnostic gold standard for the imaging of salivary glands [9]. In recent years, contrast enhanced ultrasound (CE-US) with high-resolution technique has evolved and represents a promising method to differentiate these lesions [4,7,16]. Vascularity and blood flow are indispensable parameters to analyse parenchymal organs using CE-US with high spatial and temporal resolution and high sensitivity [7].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, contrast enhanced ultrasound (CE-US) with high-resolution technique has evolved and represents a promising method to differentiate these lesions [4,7,16]. Vascularity and blood flow are indispensable parameters to analyse parenchymal organs using CE-US with high spatial and temporal resolution and high sensitivity [7]. Microbubbles, the gas-filled ultrasound contrast media with a diameter of 1-10 m, are able to circulate through the blood vessels, especially through the capillary system to enable the assessment of parenchymal perfusion.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring parotid gland tumors with a new perfusion software for contrast-enhanced ultrasound

Klotz¹,

Ingrisch²,

Eichhorn³

et al. 2014

Clinical Hemorheology and Microcirculation

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PURPOSE: Contrast enhanced ultrasound (CE-US) is a promising imaging modality for non-invasive analysis of functional vascularisation. Lesions of the parotid gland are associated with a vascularisation that differs from normal gland tissue. The aim of this clinical study was to further analyse the perfusion in parotid gland lesions with CE-US. The new quantification software VueBox (Bracco, Italy) was used to assess the perfusion, based on DICOM datasets of CE-US examination. MATERIALS AND METHODS: CE-US measurements were performed by intravenous application of a contrast agent (SonoVue, Bracco, Italy) before surgical tumor resection. From the analysis of a time sequence of 2D DICOM contrast images, area under time intensity curve (AUC), peak enhancement (PE), wash-in-rate (WiR) and wash-in-perfusion-index (WiPI) were calculated using VueBox. These were correlated with the histological analyses of the tumor tissue. RESULTS: Significant difference of area below intensity time curve (AUC), peak enhancement (PE), wash-in-rate (WiR) and wash-in perfusion index (WiPI) were observed in the malign lesions compared to benign tumors (p < 0,05) and in pleomorphic adenoma compared to cystadenolymphoma (p < 0,05). CONCLUSION: CE-US seems to be a quantitative and independent method for discriminating between malign and benign parotid gland tumors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monitoring parotid gland tumors with a new perfusion software for contrast-enhanced ultrasound

Klotz¹,

Ingrisch²,

Eichhorn³

et al. 2014

Clinical Hemorheology and Microcirculation

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“…1 They are now used in routine clinical practice for echocardiography and increasingly for other indications 2 including the quantification of biological parameters such as blood perfusion rate and hypoxia, 3 which are two important indicators of tumor malignancy. 4 Advances in the understanding of cell and tissue-specific molecular markers have also led to the development of functionalized microbubbles, which are currently being investigated for targeted molecular imaging. 5,6 Upon exposure to ultrasound, microbubbles undergo volumetric oscillations and reradiate a secondary acoustic response of significantly higher amplitude than the scattering that would be produced by a rigid sphere of equivalent size ($2 lm in diameter).…”

Section: Introductionmentioning

confidence: 99%

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

et al. 2016

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Besides their use as contrast agents for ultrasound imaging, microbubbles are increasingly studied for a wide range of therapeutic applications. In particular, their ability to enhance the uptake of drugs through the permeabilization of tissues and cell membranes shows great promise. In order to fully understand the numerous paths by which bubbles can interact with cells and the even larger number of possible biological responses from the cells, thorough and extensive work is necessary. In this review, we consider the range of experimental techniques implemented in in vitro studies with the aim of elucidating these microbubble-cell interactions. First of all, the variety of cell types and cell models available are discussed, emphasizing the need for more and more complex models replicating in vivo conditions together with experimental challenges associated with this increased complexity. Second, the different types of stabilized microbubbles and more recently developed droplets and particles are presented, followed by their acoustic or optical excitation methods. Finally, the techniques exploited to study the microbubble-cell interactions are reviewed. These techniques operate over a wide range of timescales, or even off-line, revealing particular aspects or subsequent effects of these interactions. Therefore, knowledge obtained from several techniques must be combined to elucidate the underlying processes. V C 2016 AIP Publishing LLC.

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“…They are now used in routine clinical practice for echocardiography and increasingly for other indications 2 including the quantification of biological parameters such as blood perfusion rate and hypoxia 3 , which are two important indicators of e.g. tumor malignancy 4 . Advances in the understanding of cell and tissue specific molecular markers have also led to the development of functionalized microbubbles, which are currently being widely investigated for targeted molecular imaging 5 6 .…”

Section: Introductionmentioning

confidence: 99%

Ultrasound contrast agents: bubbles drops and particles

Lajoinie¹

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The research described in this thesis is part of the research program NanoNextNL, a micro and nanotechnology consortium of the Government of the Netherlands and 130 partners. This thesis was carried out at the Physics of Fluids group of the Faculty of Science and Technology of the University of Twente. Cover design:As a representation of a crucial goal of this work, the cover depicts a confocal microscopy image showing cells selectively porated by loaded microbubbles upon ultrasound exposure. The porated cells take up a blue fluorescent probe. By Ine Lentacker, Ine De Cock and Guillaume Lajoinie. Guide through the thesisIn this thesis, we investigate different types of innovative agents, designed for biomedical use in a context of molecular imaging. In Chapter 1, we review the agents that arouse the interest of the scientific community, most of them at the research stage of development, together with the experimental methods that can be used to study their interaction with cells. Such in vitro investigations are necessary to test on short scales the impact of the various agents on biological structures.We separate the agents in three main categories. The first group consists of stabilized microbubbles, usually by means of phospholipids that fill up the interfacial area between the gas core and the surrounding liquid (water). Such bubbles are clinically used for 40 years as contrast agents for ultrasound owing to their unique resonance behavior. When irradiated with an ultrasound pulse, a bubble experiences volumetric oscillations, that reemit an ultrasound wave back to the transducer. This process makes them unchallenged ultrasound scatterers. The role of these stable microbubbles in new biomedical research directions is discussed in Chapters 2 to 9.Phospholipid coatings were developed in the last decade to do much more than just prevent bubble dissolution. They are now a crucial tool to enrich the microbubbles with specific markers or drug payloads. In Chapter 2, we investigate the behavior of this coating when the microbubbles are subjected to an ultrasound field. We show that the coating is shed away from the microbubble, which is of major interest for molecular imaging combined with drug delivery using microbubbles. We pursue the idea in Chapter 3 where we investigate how a bubble payload, here liposomes labeled with a fluorescent dye, is released under ultrasound exposure. In Chapter 4, we link these direct observations of shedding of the previous Chapters to the subsequent dissolution of the microbubbles, no longer protected by a phospholipid shell. This dissolution, visible in the ultrasound scatter spectrum can be used to remotely quantify the effect of the microbubbles. In support of these observations, we dedicate the next Chapter (Chapter 5) to physically understand the mechanisms underlying the behavior of the phospholipid coating. The application of these findings in practice requires the production a sufficiently high production rate and with great control of such microbubbles. In Chapter 6, we th...

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Perfusion characteristics of parotid gland tumors evaluated by contrast-enhanced ultrasound

Cited by 21 publications

References 19 publications

Monitoring parotid gland tumors with a new perfusion software for contrast-enhanced ultrasound

Monitoring parotid gland tumors with a new perfusion software for contrast-enhanced ultrasound

In vitro methods to study bubble-cell interactions: Fundamentals and therapeutic applications

Ultrasound contrast agents: bubbles drops and particles

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