Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering

Goh, Saik-Kia; Bertera, Suzanne; Olsen, Phillip; Candiello, Joseph; Halfter, Willi; Uechi, Guy; Balasubramani, Manimalha; Johnson, Scott A.; Sicari, Brian M.; Kollar, Elizabeth W.; Badylak, Stephen F.; Banerjee, Ipsita

doi:10.1016/j.biomaterials.2013.05.066

Cited by 256 publications

(256 citation statements)

References 65 publications

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“…3). Similar data have been recently produced in a rodent model (136). However, the successful decellularization and subsequent recellularization of the human pancreatic ECM has not been reported in the bioengineering literature.…”

Section: Ecm and Endocrine Pancreas Bioengineeringsupporting

confidence: 71%

Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

et al. 2014

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Emerging technologies in regenerative medicine have the potential to restore the b-cell compartment in diabetic patients, thereby overcoming the inadequacies of current treatment strategies and organ supply. Novel approaches include: 1) Encapsulation technology that protects islet transplants from host immune surveillance; 2) stem cell therapies and cellular reprogramming, which seek to regenerate the depleted b-cell compartment; and 3) whole-organ bioengineering, which capitalizes on the innate properties of the pancreas extracellular matrix to drive cellular repopulation. Collaborative efforts across these subfields of regenerative medicine seek to ultimately produce a bioengineered pancreas capable of restoring endocrine function in patients with insulin-dependent diabetes.Regenerative medicine promises to contribute to the advancement of b-cell replacement strategies through the development and implementation of microencapsulation technology, the production of insulin-producing cells either by regeneration from endogenous cells or reprogramming from nonendocrine adult cell sources, and, more recently, the exploitation of bioengineered microenvironments (1). Considering the shortage of available pancreata, the search for alternative cell sources has recently been categorized within one of the following three "Rs" of pancreatic b-cell replenishment: replacement (from stem cells or xenoislets), regeneration (from endogenous progenitor cells), and reprogramming (from nonendocrine adult cell sources) (2).The purpose of this article is to comprehensively and critically review the main regenerative medicine2based strategies that are currently being developed to treat type 1 diabetes. The first part of the article will illustrate and discuss islet encapsulation technology, which has been extensively investigated over the past 40 years (3) and represents one of the most promising regenerative medicine2based approaches to achieve immunoisolation of transplantable organs. The second part will focus on cell bioengineering, where different types of progenitor and differentiated cell sources are manipulated to ultimately yield insulin-producing cells. The last part of the article will summarize the most recent advancements in the study of the pancreatic extracellular matrix (ECM) as a platform for endocrine pancreas bioengineering. ISLET ENCAPSULATION TECHNOLOGYEncapsulation technology has been used to protect islets from the host immune system. This strategy holds the promise to allow implantation of islets without immunosuppression not only across genetically different individuals from the same species, but also across species barriers. MicroencapsulationThe first approach to immunoisolate cells consists of the microencapsulation of one to three islets per semipermeable immunoprotective capsule. The spherical configuration of these microcapsules results in a higher surface-tovolume ratio and a higher diffusion rate (4). Furthermore, microcapsules can be injected in large numbers, are durable, and are difficult to disrup...

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Section: Ecm and Endocrine Pancreas Bioengineeringsupporting

confidence: 71%

Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

et al. 2014

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“…70,71 A recent study of decellularized mouse pancreas identified several proteins present in the basement membrane, among them collagens I, III, V, and VI; laminins-111, 211, and 511; nidogens; and proteoglycans. 72 However, the exact role of specific ECM molecules in maintaining islet function is still under investigation. Table 1 summarizes key findings for different ECM molecules and their effect on various insulinproducing cell types.…”

Section: Ecm Cuesmentioning

confidence: 99%

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Amer

Mahoney

Bryant

2014

Tissue Engineering Part B: Reviews

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Type 1 diabetes mellitus is an autoimmune disease resulting from the destruction of insulin-producing pancreatic b-cells. Cell-based therapies, involving the transplantation of functional b-cells into diabetic patients, have been explored as a potential long-term treatment for this condition; however, success is limited. A tissue engineering approach of culturing insulin-producing cells with extracellular matrix (ECM) molecules in threedimensional (3D) constructs has the potential to enhance the efficacy of cell-based therapies for diabetes. When cultured in 3D environments, insulin-producing cells are often more viable and secrete more insulin than those in two dimensions. The addition of ECM molecules to the culture environments, depending on the specific type of molecule, can further enhance the viability and insulin secretion. This review addresses the different cell sources that can be utilized as b-cell replacements, the essential ECM molecules for the survival of these cells, and the 3D culture techniques that have been used to benefit cell function.

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“…64,65 This technology used to engineer lungs, 66 tissue grafts for heart, 67 liver 68 and pancreas in vitr. 69 Decellularization is also used in intestinal, renal and, cardiac, hepatic, skin tissue engineering ( Figure 5). …”

Section: De-cellularizationmentioning

confidence: 99%

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering

Cited by 256 publications

References 65 publications

Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

Cell Replacement Strategies Aimed at Reconstitution of the β-Cell Compartment in Type 1 Diabetes

Tissue Engineering Approaches to Cell-Based Type 1 Diabetes Therapy

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

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