Periapical lesions in rats with streptozotocin-induced diabetes

Kohsaka, Toshiaki; Kumazawa, Masahiko; Yamasaki, Masahiro; Nakamur, Hiroshi

doi:10.1016/s0099-2399(96)80243-3

Cited by 53 publications

(48 citation statements)

References 15 publications

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“…The slightly narrowing trend of the periapical lesions after 4 weeks of pulp exposure is in agreement with previous reports of two-dimensionally measured periapical lesions in Wistar rats (36,37). Four weeks of pulp exposure is regarded as the expansion term of rat periapical lesions, which stabilize thereafter (38,39).…”

Section: Discussionsupporting

confidence: 92%

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Kuremoto

Noiri

Ishimoto

et al. 2014

Appl Environ Microbiol

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bAlthough extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extraradicular biofilm-forming bacteria while investigating the effect of extraradicular biofilms on periapical lesions. Periapical lesions were induced by exposing the pulpal tissue of the mandibular first molars of male Wistar rats to their oral environment. Four weeks later, gutta-percha points were excessively inserted into the mesial root canals of the right first molars (experimental sites) but not the left first molars (control sites). After 6 and 8 weeks of pulp exposure, the presence of extraradicular biofilms was confirmed histomorphologically, and biofilm-forming bacteria were identified by using classical culture methods. The biofilms were observed in the extraradicular area of the experimental sites. Similar species were detected both inside and outside the root canals. The bacterial count, quantified by real-time PCR assays, in the extraradicular area gradually increased in the experimental sites until 20 weeks after pulp exposure. After 8 weeks of pulp exposure, the periapical lesion volume that was measured by micro-computed tomography was significantly larger in the experimental sites than in the control sites (P < 0.05 by Welch's t test). These results suggest that we developed an extraradicular biofilm model in rats and that extraradicular biofilms affect developing periapical lesions. In clinical practice, we encounter patients in whom periapical periodontitis does not heal despite a root canal treatment following general dental procedures. The etiology is considered to be the result of residual bacteria in the lateral branch of the root canal, endodontic reinfection, or both. Recent studies have shown that biofilms that form outside the apical foramen, which are termed "extraradicular biofilm," are also involved in refractory periapical periodontitis (1-4). As the bacteria in biofilms are resistant to the host's immunoreaction and to antibiotics (5), dental biofilm diseases such as caries or periodontitis have been treated by mechanical removal. However, extraradicular biofilm is impossible to remove mechanically with nonsurgical endodontic treatments. As extraradicular biofilms cannot be accessed from the root canal for mechanical removal, the only treatment methods are endodontic surgery and tooth extraction.Current research is focused on developing methods to prevent or control biofilm formation (6, 7). We recently found three analogues of N-acyl homoserine lactone that participate in quorum sensing, a system of bacterial cell-to-cell communication, and inhibit Porphyromonas gingivalis biofilm formation (8). Furthermore, azithromycin, a 15-membered macrolide antibiotic, has an antibiofilm effect on P. gingivalis (9). For clinical applicability, these in vitro effects should be exami...

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Section: Discussionsupporting

confidence: 92%

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Kuremoto

Noiri

Ishimoto

et al. 2014

Appl Environ Microbiol

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“…In the experimental group, 11 animals received intraperitoneal injections of 70 mg/kg of streptozotocin to induce diabetes mellitus (19). The remaining 11 animals were injected with a similar volume of sterile saline and served as controls.…”

Section: Methodsmentioning

confidence: 99%

“…Glucose levels directly impact the healing of periradicular lesions (18). For instance, periradicular lesions are larger in rats with streptozotocin-induced diabetes mellitus compared with normal rats (19). The infection of teeth and subsequent periapical lesion formation results in higher morbidity and mortality in nonobese diabetic mice than in healthy mice (20).…”

mentioning

confidence: 99%

The Effect of Hyperglycemia on Pulpal Healing in Rats

Garber

Shabahang

Escher

et al. 2009

Journal of Endodontics

112

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“…Previous studies have shown that the diabetic host may have an increased periapical lesion size (34) or may develop more serious infections in response to virulent root canal bacteria (18). Patients with a history of diabetes mellitus and periapical lesions may have significantly reduced healing following endodontic therapy compared with that for the nondiabetic population (17a).…”

mentioning

confidence: 99%

PCR-Based Identification of Bacteria Associated with Endodontic Infections

Fouad

Barry

Caimano³

et al. 2002

J Clin Microbiol

232

189

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PCR primers that target the bacterial 16S rRNA genes (or the tuf gene for the genus Enterococcus) were used to identify 10 putative bacterial pathogens in root canals with necrotic pulp. In addition, the associations of these microorganisms with symptoms and a history of diabetes mellitus were investigated. Microbial samples from the root canals of 24 teeth with necrotic pulp were included in the study. PCR with universal bacterial primers identified bacterial DNA in 22 specimens; the remaining 2 specimens were from intact teeth that had been traumatized 6 months prior to treatment. PCR with specific primers showed that preoperative symptoms were significantly associated with the presence of Streptococcus spp. (P < 0.001 by chi-square analysis). There was also a nonsignificant trend for symptoms to be associated with Fusobacterium nucleatum and Porphyromonas gingivalis (odds ratio, >2) and for diabetes mellitus to be associated with P. gingivalis and Porphyromonas endodontalis (odds ratio, >2). Cloning and sequencing of the universal PCR product in one specimen revealed the presence of an organism related to the genus Olsenella, which has not previously been described in endodontic infections.

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Periapical lesions in rats with streptozotocin-induced diabetes

Cited by 53 publications

References 15 publications

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

Promotion of Endodontic Lesions in Rats by a Novel Extraradicular Biofilm Model Using Obturation Materials

The Effect of Hyperglycemia on Pulpal Healing in Rats

PCR-Based Identification of Bacteria Associated with Endodontic Infections

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