Pericardial mesothelioma in a German Shepherd dog: a case report

Abstract

ABSTRACT:In this case report, a diagnosis of pericardial mesothelioma in a four year old male German shepherd dog is described. The dog, which had anorexia, bloody diarrhoea, dehydration and depression and, died on day 10 of therapy, was systematically necropsied. At necropsy, approximately 1.5 litres of cloudy and bloody exudate were detected in the thoracic cavity. The parietal lamina of the pericardium was covered with multilobular nodular masses related with each other, 1 to 5 cm in diameter and grey-yellow in colour. There were proliferations characterised with grey-yellow colour and approximately 1 to 5 mm in length on visceral pleura. The presence of abscess foci with liquefied-centrum was observed when examining a section surface of the lung. The appearance of the oesophageal serosa, thoracic aorta and the thoracic section of the diaphragm were similar to pleura. A thickening was microscopically determined in the parietal lamina of the pleura and pericardium due to papillary proliferations consisting of cells similar to cubic or cylindrical epithelium. Severe lymphocyte and plasma cell infiltrations were observed in the pleural sections next to the lung. Neoplastic cells had nuclei with large eosinophilic granular cytoplasms and large vesicular and single nucleoli. Some neoplastic cells were determined to include intracytoplasmic vacuoles. The neoplastic cells contained some mitotic figures. It was observed that some tumour cells contributed to giant cell formation through integration. In periodic acid Schiff-haematoxylin (PAS-H) examinations it was determined that the pleural basal membrane maintained its integrity. Immunohistochemically, the tumour gave a weak positive reaction with anti-pancytokeratin staining while giving intense reaction with anti-vimentin staining.

Can malignant and inflammatory pleural effusions in dogs be distinguished using computed tomography?

“…Although low in prevalence, the neoplasms that most frequently cause pleural effusion in dogs by direct seeding and invasion of the pleura are mesothelioma and carcinoma. Mesothelioma, a primary malignant neoplasm of the pleura,pericardium, or peritoneum, can be particularly difficult to diagnose on routine cytologic preparations because malignant mesothelial cells may appear similar to the reactive mesothelial cells seen with inflammatory pleural conditions . Carcinoma metastasis to the pleura can also be difficult to diagnose cytologically and may require immunocytochemical analysis of the effusion or histopathology .…”

Diffuse thoracic and peritoneal papillary mesothelioma in an adult cow: case report

“…And the authors emphasized that the analysis was done with successive tissue sections from the same paraffin block (28). In a German Shepherd dog, pericardial mesothelioma had weak cytokeratin positivity and intense vimentin reaction (6).…”

Efeito da Saccharomyces cerevisiae em cultura ou lisada na imunidade inata, na morfologia ruminal e intestinal de novilhos terminados em confinamento

“…The tissues were fixed in 10% formaldehyde for 48 h and embedded in paraffin, sectioned to make histopathological slides, stained with hematoxylin and eosin (HE), and observed under an optical microscope. According to Ceribasi et al (2014), fragments were classified according to the absence or presence of pneumonia.…”