Pericellular plasma clot negates the influence of scaffold stiffness on chondrogenic differentiation

Arora, Aditya; Kothari, Anjaney; Katti, Dhirendra S.

doi:10.1016/j.actbio.2016.09.038

Cited by 25 publications

(36 citation statements)

References 31 publications

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“…For example, chondrocytes cultured on stiff substrates (>16 kPa) displayed a spreading and polygonal shape with well-established actin fibers resulting in high cytoskeleton tension. In contrast, soft substrate (6 kPa) could provide chondrocytes with a proper living circumstance, leading to a small and round shape with compromised cytoskeleton tension to allow cellular aggregation and maintain chondrogenic phenotype by more production of cartilaginous matrix secretion [ 151 , 171 , 172 ]. Similarly, MSCs could also adopt a rounded morphology with fewer stress fibers but more cortical actins on the soft matrix ( e.g.…”

Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Liu

2022

Bioactive Materials

135

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Section: The Role Of Substrate Stiffness In Material-mediated Tissue ...mentioning

confidence: 99%

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Liu

2022

Bioactive Materials

135

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“…The gel stiffness is known to be critical in the control of cell phenotype as well as cellular aggregation and chondrogenesis [ 37 , 38 , 39 ]. The mechanical stiffness of OHA/GC/ADH/ALG hydrogel increased after secondary cross-linking ( Table 1 , Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

3D Printing of Polysaccharide-Based Self-Healing Hydrogel Reinforced with Alginate for Secondary Cross-Linking

Roh

Kim

et al. 2021

Biomedicines

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Three-dimensional (3D) bioprinting has been attractive for tissue and organ regeneration with the possibility of constructing biologically functional structures useful in many biomedical applications. Autonomous healing of hydrogels composed of oxidized hyaluronate (OHA), glycol chitosan (GC), and adipic acid dihydrazide (ADH) was achieved after damage. Interestingly, the addition of alginate (ALG) to the OHA/GC/ADH self-healing hydrogels was useful for the dual cross-linking system, which enhanced the structural stability of the gels without the loss of their self-healing capability. Various characteristics of OHA/GC/ADH/ALG hydrogels, including viscoelastic properties, cytotoxicity, and 3D printability, were investigated. Additionally, potential applications of 3D bioprinting of OHA/GC/ADH/ALG hydrogels for cartilage regeneration were investigated in vitro. This hydrogel system may have potential for bioprinting of a custom-made scaffold in various tissue engineering applications.

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“…Soft scaffolds with a compressive modulus between 0.5 kPa to 4 kPa have been shown to support in vitro chondrogenesis of MSCs. 16 The compressive modulus of multi-layered and randomly aligned scaffolds (2 kPa to 8 kPa) used in this study is in the ideal range for chondrogenesis of MSCs. 16 , 20 A few studies have attempted to improve the compressive strength of CG scaffolds by glutaraldehyde crosslinking (260 kPa) and in situ precipitation (10.2 MPa).…”

Section: Discussionmentioning

confidence: 99%

“… 16 The compressive modulus of multi-layered and randomly aligned scaffolds (2 kPa to 8 kPa) used in this study is in the ideal range for chondrogenesis of MSCs. 16 , 20 A few studies have attempted to improve the compressive strength of CG scaffolds by glutaraldehyde crosslinking (260 kPa) and in situ precipitation (10.2 MPa). 7 , 21 However, these may not be suitable for cartilage tissue engineering, as a scaffold with a compressive modulus of > 40 kPa favours bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…The quality of the in vivo regenerated tissue was also assessed by immunostaining for collagen type 2 and collagen type 10 as per a previously published protocol. 16 , 17 Three sections (at a minimum of 100 μm intervals) were taken from each specimen and processed for immunohistochemistry. Sections were incubated with primary mouse anti-collagen type 2 (II-II6B3, dilution 1:5; Developmental Studies Hybridoma Bank, Iowa City, Iowa, USA) or rabbit anti-collagen type 10 antibody (234196, dilution 1:250; Merck KGaA, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

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In vivo cartilage regeneration in a multi-layered articular cartilage architecture mimicking scaffold

Rajagopal

Ramesh

Walter

et al. 2020

Bone & Joint Research

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Aims Extracellular matrix (ECM) and its architecture have a vital role in articular cartilage (AC) structure and function. We hypothesized that a multi-layered chitosan-gelatin (CG) scaffold that resembles ECM, as well as native collagen architecture of AC, will achieve superior chondrogenesis and AC regeneration. We also compared its in vitro and in vivo outcomes with randomly aligned CG scaffold. Methods Rabbit bone marrow mesenchymal stem cells (MSCs) were differentiated into the chondrogenic lineage on scaffolds. Quality of in vitro regenerated cartilage was assessed by cell viability, growth, matrix synthesis, and differentiation. Bilateral osteochondral defects were created in 15 four-month-old male New Zealand white rabbits and segregated into three treatment groups with five in each. The groups were: 1) untreated and allogeneic chondrocytes; 2) multi-layered scaffold with and without cells; and 3) randomly aligned scaffold with and without cells. After four months of follow-up, the outcome was assessed using histology and immunostaining. Results In vitro testing showed that the secreted ECM oriented itself along the fibre in multi-layered scaffolds. Both types of CG scaffolds supported cell viability, growth, and matrix synthesis. In vitro chondrogenesis on scaffold showed an around 400-fold increase in collagen type 2 (COL2A1) expression in both CG scaffolds, but the total glycosaminoglycan (GAG)/DNA deposition was 1.39-fold higher in the multi-layered scaffold than the randomly aligned scaffold. In vivo cartilage formation occurred in both multi-layered and randomly aligned scaffolds treated with and without cells, and was shown to be of hyaline phenotype on immunostaining. The defects treated with multi-layered + cells, however, showed significantly thicker cartilage formation than the randomly aligned scaffold. Conclusion We demonstrated that MSCs loaded CG scaffold with multi-layered zonal architecture promoted superior hyaline AC regeneration. Cite this article: Bone Joint Res 2020;9(9):601–612.

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Pericellular plasma clot negates the influence of scaffold stiffness on chondrogenic differentiation

Cited by 25 publications

References 31 publications

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

3D Printing of Polysaccharide-Based Self-Healing Hydrogel Reinforced with Alginate for Secondary Cross-Linking

In vivo cartilage regeneration in a multi-layered articular cartilage architecture mimicking scaffold

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