Perichondrial Potential for Cartilagenous Regeneration

Skoog, Tord; Ohlsén, Lennart; Sohn, Stephen A.

doi:10.3109/02844317209036711

Cited by 171 publications

(67 citation statements)

References 2 publications

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“…In this study they also demcartilage and is therefore likely contaminating cultures. Undifferentiated progenitor cells in this cambium layer onstrate that the expression of MMP13 and cathepsin B was already higher in nasal chondrocytes compared to are known for their capacity for cartilage regeneration (9,36,39,42). This may explain why culturing chondroauricular chondrocytes in the monolayer condition.…”

Section: Cartilage Subtype Differentiation General Performancementioning

confidence: 99%

Differences in Cartilage-Forming Capacity of Expanded Human Chondrocytes from Ear and Nose and Their Gene Expression Profiles

et al. 2011

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The aim of this study was to evaluate the potential of culture-expanded human auricular and nasoseptal chondrocytes as cell source for regeneration of stable cartilage and to analyze the differences in gene expression profile of expanded chondrocytes from these specific locations. Auricular chondrocytes in monolayer proliferated less and more slowly (two passages took 26.7 ± 2.1 days and were reached in 4.37 ± 0.30 population doublings) than nasoseptal chondrocytes (19.3 ± 2.5 days; 5.45 ± 0.20 population doublings). However, auricular chondrocytes produced larger pellets with more cartilage-like matrix than nasoseptal chondrocytes (2.2 ± 0.71 vs. 1.7 ± 0.13 mm in diameter after 35 days of culture). Although the matrix formed by auricular and nasoseptal chondrocytes contained collagen X, it did not mineralize in an in vitro model or after in vivo subcutaneous implantation. A DNA microarray study on expanded auricular and nasoseptal chondrocytes from the same donors revealed 1,090 differentially expressed genes. No difference was observed in the expression of known markers of chondrogenic capacity (e.g., collagen II, FGFR3, BMP2, and ALK1). The most striking differences were that the auricular chondrocytes had a higher expression of anabolic growth factors BMP5 and IGF1, while matrix-degrading enzymes MMP13 and ADAMTS5 were higher expressed in nasoseptal chondrocytes. This might offer a possible explanation for the observed higher matrix production by auricular chondrocytes. Moreover, chondrocytes isolated from auricular or nasoseptal cartilage had specific gene expression profiles even after expansion. These differently expressed genes were not restricted to known characterization of donor site subtype (e.g., elastic), but were also related to developmental processes.

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Section: Cartilage Subtype Differentiation General Performancementioning

confidence: 99%

Differences in Cartilage-Forming Capacity of Expanded Human Chondrocytes from Ear and Nose and Their Gene Expression Profiles

et al. 2011

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“…From September 1986 to December 1992, 88 patients with articular cartilage defects were treated by perichondrial arthroplasty in the University Hospital, Maastricht [2,8,16,25,26]. The study was approved by the local ethics committee and was performed in accordance with the ethical standards laid down by the 1994 Declaration of Helsinki.…”

Section: Methodsmentioning

confidence: 99%

Long-term results of rib perichondrial grafts for repair of cartilage defects in the human knee

Bouwmeester

Beckers²,

Kuijer

et al. 1997

International Orthopaedics

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“…Experimental models involving cell or tissue culture of chondrocytes or chondrogenic cells have demonstrated the feasibility of growing cartilage in vitro. These include, but are not limited to, the use of chondrocytes [3,16,17], mesenchymal stem cells [ 18, 19,67] and whole tissues such as periosteum [ 15,30,35,36,42,56,57] or perichondrium [1, 2,5,7,[9][10][11][12]21,46,47,50,58,59,65] genesis. Many of these systems have relied on the use of fetal bovine serum in the medium.…”

Section: Introductionmentioning

confidence: 99%

Serum‐free media for periosteal chondrogenesis in vitro

Fitzsimmons

Sanyal

Gonzalez

et al. 2004

Journal Orthopaedic Research

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Organ culture studies involving whole explants of periosteum have been useful for studying chondrogenesis, but to date the standard culture model for these explants has required the addition of fetal bovine serum to the media. Numerous investigators have succeeded in culturing chondrocytes and embryonic cells in serum-free conditions but there have been no studies focused on achieving a defined, serum-free media for culturing periosteal explants. The purpose of the present investigation was to determine if whole periosteal explants can be grown and produce cartilage in serum-free conditions, and to define the minimum media supplements that would be conducive to chondrogenesis. 321 periosteal explants were obtained from the medial proximal tibiae of 31 two month-old NZ white rabbits and cultured using a published agarose suspension organ culture model and DMEM for six weeks. The explants were cultured with and without fetal bovine serum or bovine serum albumin and exposed to transforming growth factor beta alone, a combination of growth factors we call ChondroMix (10 ng/ml transforming growth factor beta, 50 ng/ml basic fibroblast growth factor, and 5 pg/ml growth hormone), and/or ITS+ (2.08 pg/ml each of insulin, transferrin, and selenious acid, plus 1.78 pg/ml linoleic acid and 0.42 mg/ml BSA). Maximal chondrogenic stimulation in this study was observed with the combination of ChondroMix and ITS+. However, the minimal requirement to match or exceed the level of chondrogenic stimulation seen in the standard model (TGF-1 in 100/0 FBS) was achieved simply by the addition of 2.0 pg/ml insulin in 0.10/0 BSA-containing medium (p < 0.05). Therefore, based on our results, it would be reasonable to assume that insulin is the component in ITS+ responsible for the observed increase in total cartilage growth. Lower concentrations of insulin were not effective, suggesting that the observed effect of insulin requires activation of the IGF-1 receptor.

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Perichondrial Potential for Cartilagenous Regeneration

Cited by 171 publications

References 2 publications

Differences in Cartilage-Forming Capacity of Expanded Human Chondrocytes from Ear and Nose and Their Gene Expression Profiles

Differences in Cartilage-Forming Capacity of Expanded Human Chondrocytes from Ear and Nose and Their Gene Expression Profiles

Long-term results of rib perichondrial grafts for repair of cartilage defects in the human knee

Serum‐free media for periosteal chondrogenesis in vitro

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