Pericytes in experimental MDA-MB231 tumor angiogenesis

Kurz, Haymo; Lauer, Dietrich; Papoutsi, Maria; Christ, Bodo; Wilting, Jörg

doi:10.1007/s00418-002-0388-0

Cited by 15 publications

(10 citation statements)

References 41 publications

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“…Cross reactivity can occur with plasma cells, platelets and lymphocytes 10 . The monoclonal anti-a-smooth muscle actin is specific for the single isoform a-actin that is expressed by pericytes 5 and smooth muscle cells found in the vessel wall, although KURZ et al 8 found that aSMA was not a general marker for pericytes. The integrin anb3 is a heterodimeric cell membrane adhesion molecule that plays an important role in maturation of blood vessels of the stroma 7 and is purportedly expressed by angiogenic vessels 1 .…”

Section: Resultsmentioning

confidence: 99%

The effect of the antibody used and method of quantification on oral mucosal vascularity

Elgazzar

Macluskey

Ogden

2005

International Journal of Oral and Maxillofacial Surgery

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Section: Resultsmentioning

confidence: 99%

The effect of the antibody used and method of quantification on oral mucosal vascularity

Elgazzar

Macluskey

Ogden

2005

International Journal of Oral and Maxillofacial Surgery

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“…Among normal capillaries, α-SMA is expressed in precapillaries and postcapillaries, but not midcapillaries, whereas normal venular pericytes express α-SMA [34]. Kurz et al [35] reported that pericytes expressed desmin, but not α-SMA, at the early stages of capillary sprouting in avian chorioallantoic membrane tumor angiogenesis assays. On the other hand, Morikawa et al [36] reported that pericytes in large tumors uniformly expressed both α-SMA and desmin even on capillary-size vessels in three experimental mouse tumor models.…”

Section: Discussionmentioning

confidence: 99%

Absence of Smooth Muscle Actin-Positive Pericyte Coverage of Tumor Vessels Correlates with Hematogenous Metastasis and Prognosis of Colorectal Cancer Patients

et al. 2005

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Objectives: Immature microvessels, which are not covered by pericytes, are irregular and leaky. We hypothesized that tumor cells can penetrate immature microvessels more easily than mature microvessels. In this study, we investigated the maturation of angiogenesis by the immunohistochemical staining of colorectal cancer specimens and determined the correlation between the microvessel count or the maturity of microvessels and clinicopathological variables. Methods: Ninety-two surgical specimens from our department were used. Double immunostaining of endothelial cells with anti-CD34 antibody and pericytes with anti-α-smooth muscle actin antibody was performed. The microvessel density (MVD) and microvessel pericyte coverage index (MPI) as an index of microvessel maturation were evaluated. Results: The MVD showed a significant positive correlation with tumor size, depth of invasion and Dukes’ stage. The MPI showed a significant positive correlation with the histological differentiation of the tumor tissues and distant metastasis at the time of operation. The high MVD group (≧26.0, n = 50) tended to have a poorer prognosis than the low MVD group (<26.0, n = 42) (p = 0.097). Next, the 50 patients in the high MVD group were classified into two subgroups of high MPI (≧78.1%, n = 25) and low MPI (<78.1%, n = 25). MPI showed a significant negative correlation with hematogenous metastasis, and the low MPI group demonstrated a significantly poorer survival than the high MPI group (p = 0.040). Conclusions: These findings demonstrate that immature neovascularization was observed in poorly differentiated tumors and was correlated with metastasis, resulting in a poorer prognosis. Taken together, not only microvessel density but also vascular maturation were crucial factors for colorectal cancer patients.

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“…The avian chorioallantoic membrane (CAM) is an established system for studying vascular growth and remodeling (Patan et al 1993;Kurz et al 1995Kurz et al , 2003Schlatter et al 1997;Djonov et al 2000bDjonov et al , 2002, for evaluating growth factors or biocompatible materials (Wilting et al 1993(Wilting et al , 1996Kurz et al 1998;Ribatti et al 2001;Zwadlo-Klarwasser et al 2001;Hagedorn et al 2004;Parsons-Wingerter et al 2006;Hlushchuk et al 2007;Oates et al 2007), or angiogenesis-related processes like cell or tumor growth (Papoutsi et al 2000;Kurz et al 2002Kurz et al , 2004Hagedorn et al 2005;Lugassy et al 2007). When the role of vascular mural cells during embryonic angiogenesis received increasing attention about 10 years ago, we were among the Wrst to identify the cytoskeletal proteins desmin (DES) and -smooth muscle actin ( SMA) in cells of the CAM microvasculature and connective tissue; we showed that PDGF-B may stimulate myoWbroblast formation and formation of non-capillary microvessels (Oh et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Pericytes in the mature chorioallantoic membrane capillary plexus contain desmin and α-smooth muscle actin: relevance for non-sprouting angiogenesis

Kurz

Fehr

Nitschke

et al. 2008

Histochem Cell Biol

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The chicken chorioallantoic membrane (CAM) is a frequently used tissue for studying vascular growth and remodeling, notably non-sprouting angiogenesis by formation of transluminal pillars. Vascular pericytes have received increasing attention in the field of angiogenesis research and appear important for pillar growth. Our earlier observation that desmin (DES), but not alpha-smooth muscle actin (alphaSMA) was expressed in pericytes of the mature CAM capillary plexus after E12 was confirmed by others. However, in different species or vascular beds, either marker or both have been used to identify pericytes, raising the questions if (1) expression of these cytoskeletal proteins really was mutually exclusive; or (2) different types of pericytes existed in the same vascular bed. Using triple labeling with fluorochrome-conjugated markers Sambucus nigra agglutinin, DES or alphaSMA, and DNA-specific YoPro-1, we report here for the first time a delicate filamentous, circumferentially oriented alphaSMA pattern in periendothelial cells of the mature CAM capillary plexus, quite different from the coarser, axially oriented DES pattern. A new method for automatic classification of DNA-staining pattern was applied to compare nuclei of DES- or alphaSMA-positive cells. It predicted colocalisation of both proteins in most capillary pericytes, which was confirmed by double immunostaining for DES and alphaSMA. We conclude that (1) in contrast to published work, DES and alphaSMA are not mutually exclusive in most pericytes; (2) different types of pericytes may co-exist in the same vascular bed; (3) on average, one pericyte is associated with two transluminal pillars; (4) a novel imaging modality may be useful for cell identification in angiogenesis research and elsewhere.

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Pericytes in experimental MDA-MB231 tumor angiogenesis

Cited by 15 publications

References 41 publications

The effect of the antibody used and method of quantification on oral mucosal vascularity

The effect of the antibody used and method of quantification on oral mucosal vascularity

Absence of Smooth Muscle Actin-Positive Pericyte Coverage of Tumor Vessels Correlates with Hematogenous Metastasis and Prognosis of Colorectal Cancer Patients

Pericytes in the mature chorioallantoic membrane capillary plexus contain desmin and α-smooth muscle actin: relevance for non-sprouting angiogenesis

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